GB1508086A - Method of determination of oxalic acid - Google Patents

Method of determination of oxalic acid

Info

Publication number
GB1508086A
GB1508086A GB3398476A GB3398476A GB1508086A GB 1508086 A GB1508086 A GB 1508086A GB 3398476 A GB3398476 A GB 3398476A GB 3398476 A GB3398476 A GB 3398476A GB 1508086 A GB1508086 A GB 1508086A
Authority
GB
United Kingdom
Prior art keywords
oxalic acid
formate
substance
formic acid
aug
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3398476A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IRISH STONE FOUNDATION
Original Assignee
IRISH STONE FOUNDATION
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IRISH STONE FOUNDATION filed Critical IRISH STONE FOUNDATION
Publication of GB1508086A publication Critical patent/GB1508086A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01002—Formate dehydrogenase (1.2.1.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1508086 Oxalic acid determination IRISH STONE FOUNDATION 16 Aug 1976 [15 Aug 1975 14 Nov 1975] 33984/76 Heading G1B [Also in Division C3] A method of measuring the concentration of oxalic acid, or of a metabolite capable of conversion to oxalate, in a substance comprises the steps of (a) reacting the substance with oxalate decarboxylase to convert the oxalic acid to formic acid (b) reacting the formic acid with formate dehydrogenase in the presence of NAD<SP>+</SP> (nicotinamide-adanine dinucleatide and (c) measuring formate removal so as to determine the amount of formic acid formed in (a) which is proportional to the oxalic acid content of the substance. The method is applicable to urine or serum samples in the form of a citric extract wherein formate removal is measured spectrophotometrically or fluorometrically. Oxalate decarboxylase may be prepared from Collybia velutipes and formate dehydrogenase from Pseudomonos oxalaticus. An assay kit may be provided for oxalic acid estimation.
GB3398476A 1975-08-15 1976-08-16 Method of determination of oxalic acid Expired GB1508086A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IE181275A IE42152B1 (en) 1975-08-15 1975-08-15 Method for determination of oxalic acid
IE249075 1975-11-14

Publications (1)

Publication Number Publication Date
GB1508086A true GB1508086A (en) 1978-04-19

Family

ID=26319166

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3398476A Expired GB1508086A (en) 1975-08-15 1976-08-16 Method of determination of oxalic acid

Country Status (2)

Country Link
GB (1) GB1508086A (en)
IE (1) IE42152B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2384258A1 (en) * 1977-03-18 1978-10-13 Boehringer Mannheim Gmbh PROCEDURE FOR DETERMINING FORMIATES OR COMPOUNDS CONVERTIBLE IN FORMATES AND REAGENT FOR ITS IMPLEMENTATION

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210087603A1 (en) * 2019-09-20 2021-03-25 Charite - Universitaetsmedizin Berlin Methods, kits and devices for measuring extracellular pyridine nucleotide

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2384258A1 (en) * 1977-03-18 1978-10-13 Boehringer Mannheim Gmbh PROCEDURE FOR DETERMINING FORMIATES OR COMPOUNDS CONVERTIBLE IN FORMATES AND REAGENT FOR ITS IMPLEMENTATION

Also Published As

Publication number Publication date
IE42152B1 (en) 1980-06-18
IE42152L (en) 1977-02-15

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee