GB150334A - Manufacture of specific vaccines - Google Patents

Abstract

150,334. Elektro-Osmose Akt.-Ges., (Graf Schwerin Ges.). Aug. 23, 1919, [Convention date]. Addition to 150,328. Vaccines and immunizing serums.-Specific vaccines prepared by the action of an electric current on suspensions of bacteria as described in the parent Specification are stabilized by the addition of a. protective colloid. The added colloid may be derived from immune blood serum corresponding to the particular bacteria employed so that the resulting vaccines confer passive immunity in addition to the active immunity. The colloid added is preferably a completely soluble proteid such as pseudo-globulin and albumen. In examples, normal blood serum, or immune serum is mixed with bacilli separated from a culture medium or with the whole bouillon culture and placed in the centre compartment of u three-cell electro-osmotic apparatus divided by electrodes and diaphragms, and current is passed for several hours, salt solution being added as required to maintain the conductivity. After the required period the passage of current is continued to eliminate the electrolyte and to cause the separation of euglobulin. The clear solution to be used as a vaccine is separated from the deposit of euglobulin and insoluble bacteria residue in a centrifugal apparatus. In some cases the separated deposit is dissolved in water containing electrolytes and feebly alkaline, and is diluted to form a vaccine to be used alone or mixed with the original clear solution. The bacteria specified are those of swine erysipelas and chicken cholera, and streptococci, pneumococci, and meningococci.