GB1471262A - Virus growth - Google Patents

Virus growth

Info

Publication number
GB1471262A
GB1471262A GB119973A GB119973A GB1471262A GB 1471262 A GB1471262 A GB 1471262A GB 119973 A GB119973 A GB 119973A GB 119973 A GB119973 A GB 119973A GB 1471262 A GB1471262 A GB 1471262A
Authority
GB
United Kingdom
Prior art keywords
viruses
cell strain
nutrient
mink
nutrient medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB119973A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
C VET Ltd
Original Assignee
C VET Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by C VET Ltd filed Critical C VET Ltd
Priority to GB119973A priority Critical patent/GB1471262A/en
Publication of GB1471262A publication Critical patent/GB1471262A/en
Expired legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • A61K39/23Parvoviridae, e.g. feline panleukopenia virus
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525Virus
    • A61K2039/5252Virus inactivated (killed)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/555Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55505Inorganic adjuvants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14311Parvovirus, e.g. minute virus of mice
    • C12N2750/14334Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

1471262 Growing viruses C VET Ltd 25 March 1974 [9 Jan 1973] 1199/73 Heading C6F [Also in Division A5] Feline panleucopenia or mink enteritis viruses are grown in a nutrient medium comprising a mink tissue cell strain which contains actively dividing cells. The viruses may be inactivated or attenuated by serial passage in the cell strain to produce antigenic material, which may be incorporated in a vaccine. The cell strain may be of lung, kidney or heart of an adult, or preferably, foetal mink. The cells are rendered aseptic and disaggregated mechanically or enzymatically and cultured by serial passages in a nutrient medium until a diploid or heteroploid cell strain free from extraneous viruses, is produced and the cell strain is seeded with the virus either before or after mixing with nutrient medium. Where the nutrient is added after seeding, the culture is maintained at 20-40‹C for 0À5-2 hours before adding nutrient which is added at a rate of 10-50 ml/10<SP>6</SP> viable cells, after which incubation at 30-40‹C is carried out for 5-7 days. Harvesting is achieved by centrifuging to recover extracellular virus followed by treatment to break down all walls and centrifugation to recover all associated viruses.
GB119973A 1974-03-25 1974-03-25 Virus growth Expired GB1471262A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB119973A GB1471262A (en) 1974-03-25 1974-03-25 Virus growth

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB119973A GB1471262A (en) 1974-03-25 1974-03-25 Virus growth

Publications (1)

Publication Number Publication Date
GB1471262A true GB1471262A (en) 1977-04-21

Family

ID=9717885

Family Applications (1)

Application Number Title Priority Date Filing Date
GB119973A Expired GB1471262A (en) 1974-03-25 1974-03-25 Virus growth

Country Status (1)

Country Link
GB (1) GB1471262A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0011865A1 (en) * 1978-11-30 1980-06-11 The Wellcome Foundation Limited Feline infectious peritonitis vaccine and process for its preparation
EP0011864A1 (en) * 1978-11-30 1980-06-11 The Wellcome Foundation Limited Attenuated strain of feline infectious peritonitis virus, method for preparing it and vaccine comprising it
WO1980001644A1 (en) * 1979-02-16 1980-08-21 Cornell Res Foundation Inc Heterotypic canine parvovirus vaccine
EP0027347A1 (en) * 1979-10-16 1981-04-22 Norden Laboratories, Inc. Feline infectious peritonitis virus, its preparation and vaccines containing it

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0011865A1 (en) * 1978-11-30 1980-06-11 The Wellcome Foundation Limited Feline infectious peritonitis vaccine and process for its preparation
EP0011864A1 (en) * 1978-11-30 1980-06-11 The Wellcome Foundation Limited Attenuated strain of feline infectious peritonitis virus, method for preparing it and vaccine comprising it
WO1980001644A1 (en) * 1979-02-16 1980-08-21 Cornell Res Foundation Inc Heterotypic canine parvovirus vaccine
EP0023922A1 (en) * 1979-02-16 1981-02-18 Cornell Res Foundation Inc Heterotypic canine parvovirus vaccine.
EP0023922A4 (en) * 1979-02-16 1981-10-27 Cornell Res Foundation Inc Heterotypic canine parvovirus vaccine.
EP0027347A1 (en) * 1979-10-16 1981-04-22 Norden Laboratories, Inc. Feline infectious peritonitis virus, its preparation and vaccines containing it

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee