GB1451004A - Detection of the activity of phosphorylase sensors for detecting ferromagneti - Google Patents
Detection of the activity of phosphorylase sensors for detecting ferromagnetiInfo
- Publication number
- GB1451004A GB1451004A GB406174A GB406174A GB1451004A GB 1451004 A GB1451004 A GB 1451004A GB 406174 A GB406174 A GB 406174A GB 406174 A GB406174 A GB 406174A GB 1451004 A GB1451004 A GB 1451004A
- Authority
- GB
- United Kingdom
- Prior art keywords
- glycogen
- body fluid
- mercaptoethanol
- sodium
- acetic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1451004 Isophosphorylase detection ARZNEIMITTELWERK DRESDEN VEB 29 Jan 1974 [7 Feb 1973] 04061/74 Heading GIB A process for the determination of the activity of total phosphorylase or of individual isophosphorylases in body fluids, particularly in blood, comprises contacting concentrated body fluid (or a body fluid pre-treated with starch) with a glycogen-containing substrate for phosphorylases, the enzyme activity being determined by determining the inorganic phosphates or amylose-like side chains of glycogen formed. The process may comprise mixing a concentrated body fluid with a solution containing glycylglycine, mercaptoethanol and ethylene diamine tetra acetic acid in a 1:1 ratio or body fluid pre-treated on a starch column by adsorption and washing with a solution of sodium fluoride, sodium #-glycerophosphate and mercaptoethanol (pH 6À1, 4‹C), followed by elution of the phosphorylase at 30 C and pH 6À8 with a solution of sodium #-glycerophosphate, mercaptoethanol and ethylene diamine-tetra acetic acid, and mixing the resulting fluid in a 5:3 ratio with a glycogen-containing solution (the substrate), then adding 2 parts of a solution containing glucose-1-phosphate and adenosine monophosphate, the mixture being incubated for 60 minutes, thereafter denaturing with trichloroacetic acid, the phosphate content being subsequently determined. An alternative process comprises pretreating a body fluid on a starch column as above, diluting the eluate with 1À2M saccharose in a 2:1 ratio, applying the diluted eluate to an acrylamide separation gel containing 0À1% glycogen (the substrate) and buffered with a solution containing tris-(hydroxymethyl)-amino methane, ethylenediamine-tetra acetic acid and borric acid, separating by disc electro-phoreses and subsequently, after incubation in an aqueous solution containing glucose-1-phosphate, adenosine monophosphate, sodium #-glycerophosphate, ethylene diaminetetra acetic acid, mercaptoethanol and sodium fluoride, the formation of the amylose-like side chains on the glycogen being made visible by iodine-potassium iodide coloration.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DD16876573A DD101986A1 (en) | 1973-02-07 | 1973-02-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1451004A true GB1451004A (en) | 1976-09-29 |
Family
ID=5490033
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB406174A Expired GB1451004A (en) | 1973-02-07 | 1974-01-29 | Detection of the activity of phosphorylase sensors for detecting ferromagneti |
Country Status (9)
| Country | Link |
|---|---|
| BG (1) | BG20730A1 (en) |
| CS (1) | CS195756B1 (en) |
| DD (1) | DD101986A1 (en) |
| DE (1) | DE2401484A1 (en) |
| FR (1) | FR2327545A1 (en) |
| GB (1) | GB1451004A (en) |
| HU (1) | HU169957B (en) |
| PL (1) | PL94983B1 (en) |
| SU (1) | SU976958A1 (en) |
-
1973
- 1973-02-07 DD DD16876573A patent/DD101986A1/xx unknown
-
1974
- 1974-01-12 DE DE19742401484 patent/DE2401484A1/en not_active Withdrawn
- 1974-01-29 GB GB406174A patent/GB1451004A/en not_active Expired
- 1974-02-05 BG BG2570174A patent/BG20730A1/xx unknown
- 1974-02-06 SU SU741999385A patent/SU976958A1/en active
- 1974-02-06 HU HUAE000406 patent/HU169957B/hu unknown
- 1974-02-07 FR FR7404148A patent/FR2327545A1/en active Granted
- 1974-02-07 CS CS85674A patent/CS195756B1/en unknown
- 1974-02-07 PL PL16863774A patent/PL94983B1/pl unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE2401484A1 (en) | 1974-08-29 |
| BG20730A1 (en) | 1975-12-20 |
| HU169957B (en) | 1977-03-28 |
| SU976958A1 (en) | 1982-11-30 |
| PL94983B1 (en) | 1977-09-30 |
| DD101986A1 (en) | 1973-11-20 |
| FR2327545B1 (en) | 1978-02-17 |
| CS195756B1 (en) | 1980-02-29 |
| FR2327545A1 (en) | 1977-05-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kundig et al. | Phosphate bound to histidine in a protein as an intermediate in a novel phospho-transferase system | |
| Reeves et al. | Pyrophosphate: D-fructose 6-phosphate 1-phosphotransferase: a new enzyme with the glycolytic function of 6-phosphofructokinase | |
| Darrow et al. | [25] Hexokinase from Baker's yeast: ATP+ Hexose→ ADP+ Hexose-6-phosphate+ H+ | |
| HAECKEL et al. | Purification and allosteric properities of yeast pyruvate kinase | |
| Levitzki et al. | Role of an allosteric effector. Guanosine triphosphate activation in cytosine triphosphate synthetase | |
| GB1512328A (en) | Method and reagent for determining the activity of creatinekinase-mb | |
| Switzer et al. | [1] Phosphoribosylprophosphate synthetase (ribose-5-phosphate pyrophosphokinase) from Salmonella typhimurium | |
| Yamada et al. | Purification and properties of adenosine kinase from rat brain | |
| GB1163409A (en) | Laboratory Reagent for Assay of Creatine Phosphokinase | |
| Agarwal et al. | Identification and isolation on a large scale of guanylate kinase from human erythrocytes: effects of monophosphate nucleotides of purine analogs | |
| Putnins et al. | Colorimetric determination of inorganic pyrophosphate by a manual or automated method | |
| Koler et al. | The mechanism of precursor modulation of human pyruvate kinase I by fructose diphosphate | |
| Wood | Chapter IX Assay of Enzymes Representative of Metabolic Pathways | |
| Greenberg et al. | Further studies on N5-formyltetrahydrofolic acid cyclodehydrase | |
| Porter et al. | Purification and kinetic characterization of a specific glucokinase from Streptococcus mutans OMZ70 cells | |
| Williams et al. | [54] Aspartase | |
| Mockrin et al. | Subunit interactions in yeast glyceraldehyde-3-phosphate dehydrogenase | |
| GB1451004A (en) | Detection of the activity of phosphorylase sensors for detecting ferromagneti | |
| Glaser et al. | Preparation and properties of phosphomannomutase from baker's yeast | |
| Kiel et al. | Identification, separation, and preliminary characterization of invertase and beta-galactosidase in Actinomyces viscosus | |
| Small et al. | Purification and properties of nucleoside tetraphosphate hydrolase from rabbit muscle | |
| Bostick et al. | Methodologies for the determination of adenosine phosphates | |
| Wang | [66] Hydrolytic nucleosidases | |
| US4812398A (en) | Reagent for measuring amylase activity and measuring method thereof | |
| Albrecht et al. | Folate reductase and specific dihydrofolate reductase of the amethopterin-sensitive Streptococcus faecium var durans |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |