GB1451004A - Detection of the activity of phosphorylase sensors for detecting ferromagneti - Google Patents
Detection of the activity of phosphorylase sensors for detecting ferromagnetiInfo
- Publication number
- GB1451004A GB1451004A GB406174A GB406174A GB1451004A GB 1451004 A GB1451004 A GB 1451004A GB 406174 A GB406174 A GB 406174A GB 406174 A GB406174 A GB 406174A GB 1451004 A GB1451004 A GB 1451004A
- Authority
- GB
- United Kingdom
- Prior art keywords
- glycogen
- body fluid
- mercaptoethanol
- sodium
- acetic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000009097 Phosphorylases Human genes 0.000 title abstract 4
- 108010073135 Phosphorylases Proteins 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title abstract 2
- 210000001124 body fluid Anatomy 0.000 abstract 6
- 239000010839 body fluid Substances 0.000 abstract 6
- 239000000243 solution Substances 0.000 abstract 6
- 229920002527 Glycogen Polymers 0.000 abstract 5
- 229940096919 glycogen Drugs 0.000 abstract 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 abstract 4
- 229960001484 edetic acid Drugs 0.000 abstract 4
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 abstract 4
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 abstract 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 abstract 4
- 229920002472 Starch Polymers 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 229910052708 sodium Inorganic materials 0.000 abstract 3
- 239000011734 sodium Substances 0.000 abstract 3
- 235000019698 starch Nutrition 0.000 abstract 3
- 239000008107 starch Substances 0.000 abstract 3
- 239000000758 substrate Substances 0.000 abstract 3
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 abstract 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 2
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 abstract 2
- LNQVTSROQXJCDD-UHFFFAOYSA-N adenosine monophosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)C(OP(O)(O)=O)C1O LNQVTSROQXJCDD-UHFFFAOYSA-N 0.000 abstract 2
- HXXFSFRBOHSIMQ-VFUOTHLCSA-N alpha-D-glucose 1-phosphate Chemical compound OC[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O HXXFSFRBOHSIMQ-VFUOTHLCSA-N 0.000 abstract 2
- 229950010772 glucose-1-phosphate Drugs 0.000 abstract 2
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 abstract 2
- 239000011775 sodium fluoride Substances 0.000 abstract 2
- 235000013024 sodium fluoride Nutrition 0.000 abstract 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 108010008488 Glycylglycine Proteins 0.000 abstract 1
- DKNPRRRKHAEUMW-UHFFFAOYSA-N Iodine aqueous Chemical compound [K+].I[I-]I DKNPRRRKHAEUMW-UHFFFAOYSA-N 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 229930006000 Sucrose Natural products 0.000 abstract 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 235000013681 dietary sucrose Nutrition 0.000 abstract 1
- 238000007865 diluting Methods 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 229940043257 glycylglycine Drugs 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 235000021317 phosphate Nutrition 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 abstract 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1451004 Isophosphorylase detection ARZNEIMITTELWERK DRESDEN VEB 29 Jan 1974 [7 Feb 1973] 04061/74 Heading GIB A process for the determination of the activity of total phosphorylase or of individual isophosphorylases in body fluids, particularly in blood, comprises contacting concentrated body fluid (or a body fluid pre-treated with starch) with a glycogen-containing substrate for phosphorylases, the enzyme activity being determined by determining the inorganic phosphates or amylose-like side chains of glycogen formed. The process may comprise mixing a concentrated body fluid with a solution containing glycylglycine, mercaptoethanol and ethylene diamine tetra acetic acid in a 1:1 ratio or body fluid pre-treated on a starch column by adsorption and washing with a solution of sodium fluoride, sodium #-glycerophosphate and mercaptoethanol (pH 6À1, 4‹C), followed by elution of the phosphorylase at 30 C and pH 6À8 with a solution of sodium #-glycerophosphate, mercaptoethanol and ethylene diamine-tetra acetic acid, and mixing the resulting fluid in a 5:3 ratio with a glycogen-containing solution (the substrate), then adding 2 parts of a solution containing glucose-1-phosphate and adenosine monophosphate, the mixture being incubated for 60 minutes, thereafter denaturing with trichloroacetic acid, the phosphate content being subsequently determined. An alternative process comprises pretreating a body fluid on a starch column as above, diluting the eluate with 1À2M saccharose in a 2:1 ratio, applying the diluted eluate to an acrylamide separation gel containing 0À1% glycogen (the substrate) and buffered with a solution containing tris-(hydroxymethyl)-amino methane, ethylenediamine-tetra acetic acid and borric acid, separating by disc electro-phoreses and subsequently, after incubation in an aqueous solution containing glucose-1-phosphate, adenosine monophosphate, sodium #-glycerophosphate, ethylene diaminetetra acetic acid, mercaptoethanol and sodium fluoride, the formation of the amylose-like side chains on the glycogen being made visible by iodine-potassium iodide coloration.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DD16876573A DD101986A1 (en) | 1973-02-07 | 1973-02-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1451004A true GB1451004A (en) | 1976-09-29 |
Family
ID=5490033
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB406174A Expired GB1451004A (en) | 1973-02-07 | 1974-01-29 | Detection of the activity of phosphorylase sensors for detecting ferromagneti |
Country Status (9)
| Country | Link |
|---|---|
| BG (1) | BG20730A1 (en) |
| CS (1) | CS195756B1 (en) |
| DD (1) | DD101986A1 (en) |
| DE (1) | DE2401484A1 (en) |
| FR (1) | FR2327545A1 (en) |
| GB (1) | GB1451004A (en) |
| HU (1) | HU169957B (en) |
| PL (1) | PL94983B1 (en) |
| SU (1) | SU976958A1 (en) |
-
1973
- 1973-02-07 DD DD16876573A patent/DD101986A1/xx unknown
-
1974
- 1974-01-12 DE DE19742401484 patent/DE2401484A1/en not_active Withdrawn
- 1974-01-29 GB GB406174A patent/GB1451004A/en not_active Expired
- 1974-02-05 BG BG2570174A patent/BG20730A1/xx unknown
- 1974-02-06 SU SU741999385A patent/SU976958A1/en active
- 1974-02-06 HU HUAE000406 patent/HU169957B/hu unknown
- 1974-02-07 PL PL16863774A patent/PL94983B1/pl unknown
- 1974-02-07 FR FR7404148A patent/FR2327545A1/en active Granted
- 1974-02-07 CS CS85674A patent/CS195756B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE2401484A1 (en) | 1974-08-29 |
| PL94983B1 (en) | 1977-09-30 |
| DD101986A1 (en) | 1973-11-20 |
| CS195756B1 (en) | 1980-02-29 |
| BG20730A1 (en) | 1975-12-20 |
| FR2327545A1 (en) | 1977-05-06 |
| SU976958A1 (en) | 1982-11-30 |
| FR2327545B1 (en) | 1978-02-17 |
| HU169957B (en) | 1977-03-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |