GB1407838A - Biological cell scanning system - Google Patents
Biological cell scanning systemInfo
- Publication number
- GB1407838A GB1407838A GB4713472A GB4713472A GB1407838A GB 1407838 A GB1407838 A GB 1407838A GB 4713472 A GB4713472 A GB 4713472A GB 4713472 A GB4713472 A GB 4713472A GB 1407838 A GB1407838 A GB 1407838A
- Authority
- GB
- United Kingdom
- Prior art keywords
- cell
- cells
- sample
- nucleus
- video
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000004027 cell Anatomy 0.000 abstract 20
- 210000000805 cytoplasm Anatomy 0.000 abstract 7
- 239000010453 quartz Substances 0.000 abstract 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 3
- 238000012360 testing method Methods 0.000 abstract 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 2
- 230000003287 optical effect Effects 0.000 abstract 2
- 238000012545 processing Methods 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000000862 absorption spectrum Methods 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 230000004069 differentiation Effects 0.000 abstract 1
- 238000000295 emission spectrum Methods 0.000 abstract 1
- 235000011187 glycerol Nutrition 0.000 abstract 1
- 238000011835 investigation Methods 0.000 abstract 1
- 230000001788 irregular Effects 0.000 abstract 1
- 230000031700 light absorption Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000005855 radiation Effects 0.000 abstract 1
- 238000011160 research Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 238000004088 simulation Methods 0.000 abstract 1
- 238000012546 transfer Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Image Processing (AREA)
Abstract
1407838 Investigating biological cells photo-electrically; automatic analysis; sample transfer NUCLEAR RESEARCH ASSOCIATES Inc 12 Oct 1972 47134/72 Headings G1A and G1B Biological cells are investigated photo-electrically as they sequentially pass a scanning station. Various characteristics are measured on the basis of the times at which a light beam scanned across a cell detects the edges of the cell and of the nucleus by a data processing apparatus and recorded on a video recorder. Counts are made of cells in various categories. The recorded video signals can be played back so that particular cells can be displayed on a microscope monitor for detailed examination. Scanning system, Figs.6 and 7. Prepared samples from different persons are fed sequentially to quartz capillary tube 46 by an automatic sample handling system 30 using a conveyer belt system, a sample tube elevator and magnetic stirrers, and is detailed in Fig. 25. Each sample is supplied to the tube through syringe 98, provided with various sealing arrangements, Fig. 7. The capillary is illuminated by a flying spot scanner 82 having a phosphor screen 84 which emits U.V. and visible light, but mainly the former. Examples of suitable phosphors are given, together with a typical emission spectrum Fig. 3 (not shown) and an absorption spectrum for a typical cell Fig. 2 (not shown) (nucleus absorbs U.V. more than cytoplasm does). The scanner carries out a raster scan, control circuiting therefore being described, Figs. 4-6 (not shown). The radiation omitted from the scanner is focused within the tube 46 by quartz optical system 85, a drop of glycerine of the same r.i. as the rest of the optics being used to prevent distortion of the light spot. A quartz objective 86 focuses light on to the detector system which includes two photomultipliers and a filter and beam splitting system to provide two outputs, one representing U.V. absorption and the other visible light absorption. In video conditioner circuit 70, detailed in Fig. 8 (not shown), the visible video is subtracted from the U.V. video, in order to (a) increase signal/ noise ratio (b) distinguish cells from non-cellular material (c) enhance nucleus/cytoplasm differentiation. The signal is then applied to a switch which eliminates those portions of the video signal representative of tube walls, before application to the Data processing apparatus. Figs. 9-21 (not shown). This performs five tests on video signals which include nucleus and cytoplasm components: (a) diameter of nucleus should be below a predetermined threshold; (b) cytoplasm shoulders should be above a certain threshold width; (c) ratio of nucleus/cytoplasm should be below certain threshold; (d) cytoplasm shoulders should be consistently narrow or broad, i.e. cell not of irregular shape; (e) the integrated density of nucleus should be below a predetermined threshold. The visible video signal is used independently to determine whether a cell is a histocyte e.g. a living cell, which contains more carbon, which absorbs visible light, than normal. Display, recording and playback facilities. The microscope monitor 700 is used to display the scanned area and allows (a) optimising of optical set-up, focusing &c. (b) annotation - two columns on the left of the display indicate "histocyte" or "normal" and five on the right indicate the results of the five tests and (c) simulation of a cell in order to aid setting up of discriminator levels in the processor. Figs.23 and 28 (not shown). Cells are displayed in three levels of contrast against a white background. Since they move quickly during normal sample investigation, a further (recorder) monitor is provided to provide an equivalent display of the video tape recording, when an individual cell can be electronically held stationary for examination. Also provided are system and recorder oscilloscopes for examination of real-time and recorded line scans at various points in the system. Counter bank 40 and Fig. 24 (not shown). This comprises a sample counter, which identifies the number of the sample; a cell counter, which in association with a counter storing a pre-set number, indicates whether a sufficient number of cells have been counted; a non-normal counter which counts the number of cells failing one or more of the five tests; an ambiguous counter which counts the number of occurrences of scans of e.g. a clump of cells which gives too many nucleus or cytoplasm components in a single video line scan; a histrocyte counter, indicating the fact that a sample is a living sample. If sufficient cells have been counted, the counts are read into data acquisition system. Alternative arrangements. The source may be a laser. The U.V. and visible signals may be combined other than by subtraction. Samples may be stained; may be blood samples, for identification of white cell types.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19722242563 DE2242563A1 (en) | 1972-08-30 | 1972-08-30 | ARRANGEMENT FOR ANALYSIS OF BIOLOGICAL CELLS |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1407838A true GB1407838A (en) | 1975-09-24 |
Family
ID=5854955
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB4713472A Expired GB1407838A (en) | 1972-08-30 | 1972-10-12 | Biological cell scanning system |
Country Status (5)
Country | Link |
---|---|
CA (2) | CA991438A (en) |
CH (1) | CH585904A5 (en) |
DE (1) | DE2242563A1 (en) |
FR (1) | FR2198632A5 (en) |
GB (1) | GB1407838A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0160568A2 (en) * | 1984-05-01 | 1985-11-06 | Ortho Diagnostic Systems, Inc. | Methods and apparatus for analysis of particles and cells |
CN113640195A (en) * | 2021-02-03 | 2021-11-12 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994010564A1 (en) * | 1992-11-05 | 1994-05-11 | Evotec Biosystems Gmbh | Process for separating substances from dilute solutions and suspensions |
US5733507A (en) * | 1995-06-07 | 1998-03-31 | Inphocyte, Inc. | Biological cell sample holder for use in infrared and/or Raman spectroscopy analysis holder |
US5733739A (en) * | 1995-06-07 | 1998-03-31 | Inphocyte, Inc. | System and method for diagnosis of disease by infrared analysis of human tissues and cells |
US5848977A (en) * | 1996-02-16 | 1998-12-15 | Inphocyte, Inc. | Sample holder for cells |
US20150269314A1 (en) | 2014-03-20 | 2015-09-24 | Rudjer Boskovic Institute | Method and apparatus for unsupervised segmentation of microscopic color image of unstained specimen and digital staining of segmented histological structures |
-
1972
- 1972-08-30 DE DE19722242563 patent/DE2242563A1/en active Pending
- 1972-09-06 FR FR7231583A patent/FR2198632A5/fr not_active Expired
- 1972-10-03 CH CH1455472A patent/CH585904A5/xx not_active IP Right Cessation
- 1972-10-12 GB GB4713472A patent/GB1407838A/en not_active Expired
- 1972-10-16 CA CA153,963A patent/CA991438A/en not_active Expired
-
1976
- 1976-04-15 CA CA250,384A patent/CA1036385A/en not_active Expired
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0160568A2 (en) * | 1984-05-01 | 1985-11-06 | Ortho Diagnostic Systems, Inc. | Methods and apparatus for analysis of particles and cells |
EP0160568A3 (en) * | 1984-05-01 | 1987-04-15 | Ortho Diagnostic Systems Inc. | Methods and apparatus for analysis of particles and cells |
CN113640195A (en) * | 2021-02-03 | 2021-11-12 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
CN113640195B (en) * | 2021-02-03 | 2022-10-11 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
Also Published As
Publication number | Publication date |
---|---|
CA1036385A (en) | 1978-08-15 |
FR2198632A5 (en) | 1974-03-29 |
CA991438A (en) | 1976-06-22 |
CH585904A5 (en) | 1977-03-15 |
DE2242563A1 (en) | 1974-03-21 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
PCNP | Patent ceased through non-payment of renewal fee |