GB1407150A - Carrier-bound proteins - Google Patents

Carrier-bound proteins

Info

Publication number
GB1407150A
GB1407150A GB5602473A GB5602473A GB1407150A GB 1407150 A GB1407150 A GB 1407150A GB 5602473 A GB5602473 A GB 5602473A GB 5602473 A GB5602473 A GB 5602473A GB 1407150 A GB1407150 A GB 1407150A
Authority
GB
United Kingdom
Prior art keywords
carrier
functional group
protein
aqueous solution
bound proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5602473A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19712128743 external-priority patent/DE2128743C3/en
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of GB1407150A publication Critical patent/GB1407150A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F289/00—Macromolecular compounds obtained by polymerising monomers on to macromolecular compounds not provided for in groups C08F251/00 - C08F287/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/084—Polymers containing vinyl alcohol units
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/098—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer formed in the presence of the enzymes or microbial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/18—Multi-enzyme systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin

Abstract

1407150 Carrier-bound proteins BOEHRINGER MANNHEIM GmbH 4 Dec 1973 [8 Dec 1972] 56024/73 Addition to 1357861 Heading C3H A process for the production of carrier-bound proteins, e.g. the enzymes glucose oxidase, trypsin and hexokinase wherein a protein is reacted in aqueous solution with a coupling compound which contains at least one group capable of acylating or alkylating proteins and at least one further functional group capable of producing a bond with a carrier substance whereafter this further functional group is reacted with the carrier substance. The functional group capable of reacting with the protein may be an ethylene imine group, a halide group activated by unsaturation, an acid halide, an azide, an acid anhydride, an aldehyde or an oxazolone. The further functional group is preferably a polymerizable double bond. The carrier may be produced in the aqueous solution or suspension by the polymerization of at least one monomer, e.g. a derivative of acrylic acid, methacrylic acid or vinyl alcohol or a mixture of said derivatives, and the carrier may be crosslinked after the production thereof. In a preferred embodiment the polymerizable monomer or monomer mixture is added to the aqueous solution after the reaction of the protein with the coupling compound and is then polymerized.
GB5602473A 1971-06-09 1973-12-04 Carrier-bound proteins Expired GB1407150A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19712128743 DE2128743C3 (en) 1971-06-09 Process for the production of carrier-bound protein
DE2260185A DE2260185C3 (en) 1971-06-09 1972-12-08 Carrier-bound protein and method for its production - US Pat

Publications (1)

Publication Number Publication Date
GB1407150A true GB1407150A (en) 1975-09-24

Family

ID=25761260

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5602473A Expired GB1407150A (en) 1971-06-09 1973-12-04 Carrier-bound proteins

Country Status (4)

Country Link
DE (1) DE2260185C3 (en)
FR (1) FR2209775B2 (en)
GB (1) GB1407150A (en)
NL (1) NL175194C (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2631656C2 (en) * 1976-07-14 1983-02-10 Boehringer Mannheim Gmbh, 6800 Mannheim Use of a hydroxysuccinimido ester derivative for binding biologically active proteins to one another and / or to liquid or solid carriers
DE2708018A1 (en) * 1977-02-24 1978-09-07 Boehringer Mannheim Gmbh BIOLOGICALLY ACTIVE PROTEIN FIXED TO POLYAMIDE AND THE PROCESS FOR ITS PRODUCTION

Also Published As

Publication number Publication date
NL175194C (en) 1984-10-01
FR2209775B2 (en) 1978-07-07
NL7314673A (en) 1974-06-11
DE2260185A1 (en) 1974-07-11
DE2260185B2 (en) 1978-10-19
FR2209775A2 (en) 1974-07-05
DE2260185C3 (en) 1979-06-13
NL175194B (en) 1984-05-01

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]