GB1396964A - Production of enzymes - Google Patents

Production of enzymes

Info

Publication number
GB1396964A
GB1396964A GB3514972A GB3514972A GB1396964A GB 1396964 A GB1396964 A GB 1396964A GB 3514972 A GB3514972 A GB 3514972A GB 3514972 A GB3514972 A GB 3514972A GB 1396964 A GB1396964 A GB 1396964A
Authority
GB
United Kingdom
Prior art keywords
petri dishes
series
homologous series
homologous
strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3514972A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bpifrance Financement SA
Original Assignee
Agence National de Valorisation de la Recherche ANVAR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agence National de Valorisation de la Recherche ANVAR filed Critical Agence National de Valorisation de la Recherche ANVAR
Publication of GB1396964A publication Critical patent/GB1396964A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

1396964 Detergents containing enzymes AGENCE NATIONALE DE VALORISATION DE LA RECHERCHE 27 July 1972 [28 July 1971] 35149/72 Heading C5D [Also in Division C3 and C6] A household detergent contains a proteolytic enzyme prepared by culturing an neperogenic bacterial strain (particularly that deposited in the culture collection of the Pasteur Institute in Paris under accession number 71-I-001) and recovering the proteuse (from the culture medium) by known methods. The asperogenic bacteria to be employed are selected by the following procedure based on the discovery that the non-aperulated cells of Bacillus subtilis are not resistant to chloroform vapour shereno the apores are resistant: (a) mutating, with N- methyl-N<SP>1</SP>-nitro-N-nitrosoguanidine, germinated spores of wild Bacillus subtilis and spreading the spores subjected to the mutagenic treatment over an agar-containing nutrient broth accommodated in a main series of Petri dishes to obtain after incubation colonies containing spores; (b) replicating each Petri dish of the main series to obtain a first homologous series of Petri dishes; (c) submitting each Petri dish of the main series to chloroform vapour and replicating it to obtain a second homologous series of Petri dishes is (d) incubating the first homologous series and the second homologous series of Petri dishes; (e) by comparison between homologous Petri dishes of the first homologous series and the second homologous series, selecting the neperogenic mutant strains which are strains of the Petri dishes of the first homologous series that are not in the Petri dishes of the second homologous series; and testing the proteolytic activity of these selected strains, using a nutrient medium with azo-casein added, and selecting strains having similar or proteolytic activity to the wild strain.
GB3514972A 1971-07-28 1972-07-27 Production of enzymes Expired GB1396964A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7127671A FR2147470A5 (en) 1971-07-28 1971-07-28

Publications (1)

Publication Number Publication Date
GB1396964A true GB1396964A (en) 1975-06-11

Family

ID=9081077

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3514972A Expired GB1396964A (en) 1971-07-28 1972-07-27 Production of enzymes

Country Status (8)

Country Link
US (1) US3833474A (en)
JP (1) JPS4835078A (en)
BE (1) BE786826A (en)
DE (1) DE2236926A1 (en)
FR (1) FR2147470A5 (en)
GB (1) GB1396964A (en)
IT (1) IT968106B (en)
NL (1) NL7210486A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1142105A (en) * 1978-07-04 1983-03-01 Peter Tang Protease product of reduced allergenicity

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1303633A (en) * 1969-04-18 1973-01-17
US3740318A (en) * 1971-02-19 1973-06-19 Upjohn Co Composition of matter and process

Also Published As

Publication number Publication date
US3833474A (en) 1974-09-03
IT968106B (en) 1974-03-20
DE2236926A1 (en) 1973-02-08
BE786826A (en) 1972-11-16
NL7210486A (en) 1973-01-30
JPS4835078A (en) 1973-05-23
FR2147470A5 (en) 1973-03-09

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PLNP Patent lapsed through nonpayment of renewal fees