GB1293563A - Enzymes and methods for their preparation, isolation and purification - Google Patents

Enzymes and methods for their preparation, isolation and purification

Info

Publication number
GB1293563A
GB1293563A GB917970A GB917970A GB1293563A GB 1293563 A GB1293563 A GB 1293563A GB 917970 A GB917970 A GB 917970A GB 917970 A GB917970 A GB 917970A GB 1293563 A GB1293563 A GB 1293563A
Authority
GB
United Kingdom
Prior art keywords
mixture
enzyme
proteolytic
enzyme mixture
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB917970A
Inventor
Per Staffan Delin
Karl Hans Fritz Kiessling
Lars Solve Nathorstwestfelt
Karl Hugo Thelin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Astra Lakemedel AB
Original Assignee
Astra Lakemedel AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Astra Lakemedel AB filed Critical Astra Lakemedel AB
Priority to FI700770A priority Critical patent/FI47779C/en
Priority to FI00770/70A priority patent/FI47779B/fi
Priority to SE04417/70A priority patent/SE362252B/xx
Priority to BE748297D priority patent/BE748297A/en
Priority to US25454A priority patent/US3684658A/en
Priority to AU13462/70A priority patent/AU1346270A/en
Priority to DE19702016822 priority patent/DE2016822A1/en
Priority to NL7005169A priority patent/NL7005169A/xx
Publication of GB1293563A publication Critical patent/GB1293563A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)

Abstract

1293563 Hydrolytic enzyme mixture ASTRA LAKEMEDEL AB 6 April 1970 [10 April 1969 25 Feb 1970] 18582/69 and 9179/70 Heading C3H A stable hydrolytic enzyme mixture which is a mixture of proteins is characterized in that it (a) has a U.V. spectrum characteristic of proteins containing aromatic amino acids, (b) is proteolytically active against casein, haemoglobin and gelatine, with a proteolytic optimum at pH 9-10 with casein, (c) hydrolyses N-α- tosyl-L-arginine ethyl ester, (d) contains proteolytic activity inducable by heating, (e) also contains esterases, lipases and amylases, (f) precipitates a heat stable enzyme with organic solvents (e.g. acetone) or ammonium sulphate, and (g) has stability and activity independent of Ca<SP>++</SP> ion concentration in solution. The mixture may be formed by fermenting Streptomyces globisporus variant NRRL 3762 under submerged aerobic conditions in a nutrient medium at, e.g. 26-30‹ C. and an initial pH of 6À0-7À5. A proteolytic enzyme fraction may be obtained from the enzyme mixture by subjecting it to gel filtration using a modified dextran having a fractionation range for peptides and globular proteins of molecular weight 1500-30,000 and eluting the dextran with water, or by contacting a solution of the enzymes with diethylaminoethyl cellulose and eluting the cellulose with ammonium acetate at pH 7À4, or by subjecting the enzyme mixture to isoelectric separation and recovering the fraction eluted at pH 4-6.
GB917970A 1969-04-10 1970-02-25 Enzymes and methods for their preparation, isolation and purification Expired GB1293563A (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FI700770A FI47779C (en) 1969-04-10 1970-03-19 Method for preparing a hydrolytic enzyme mixture.
FI00770/70A FI47779B (en) 1969-04-10 1970-03-19
SE04417/70A SE362252B (en) 1969-04-10 1970-03-31
BE748297D BE748297A (en) 1969-04-10 1970-04-01 ENZYMES AND METHOD FOR THE PREPARATION, ISOLATION AND PURIFICATION OF THESE ENZYMES
US25454A US3684658A (en) 1969-04-10 1970-04-03 Enzymes and process for their preparation
AU13462/70A AU1346270A (en) 1969-04-10 1970-04-06 Enzymes and methods for their preparation, isolation and purification
DE19702016822 DE2016822A1 (en) 1969-04-10 1970-04-09 Enzyme mixture and process for its preparation
NL7005169A NL7005169A (en) 1969-04-10 1970-04-10

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1858269 1969-04-10

Publications (1)

Publication Number Publication Date
GB1293563A true GB1293563A (en) 1972-10-18

Family

ID=10114925

Family Applications (1)

Application Number Title Priority Date Filing Date
GB917970A Expired GB1293563A (en) 1969-04-10 1970-02-25 Enzymes and methods for their preparation, isolation and purification

Country Status (2)

Country Link
FR (1) FR2054547A1 (en)
GB (1) GB1293563A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56106593A (en) * 1980-01-24 1981-08-24 Dainippon Pharmaceut Co Ltd Alkali protease m3

Also Published As

Publication number Publication date
FR2054547B1 (en) 1973-03-16
FR2054547A1 (en) 1971-04-23

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Legal Events

Date Code Title Description
PS Patent sealed
PLNP Patent lapsed through nonpayment of renewal fees