GB1249476A - Through-flow reactor - Google Patents

Abstract

1,249,476. Automatic analysis; contacting liquids; liquid reaction chromatography. CESKOSLOVENSKA AKADEMIE VED. 10 Oct., 1968, No. 48115/68. Headings B1F and B1X. A method for separating and determining dissolved substances carried in a flowing liquid medium comprises chromatographically separating the substances in column 23 (Fig. 2) and thereafter reacting the separated substances within the flowing medium with chemical reagent forming dyestuff, the reaction taking place in tube 2 (Fig. 1) having an inlet and an outlet, in the tube a chemically inert packing containing multiplicity of capillary channels, for example glass or polytetrafluoroethylene spheres, and at least the outlet end of the tube having a closure bearing on the packing in the tube, the concentrations of dyestuff formed being determined, for example in photometer 29. The tube may be integral with the column, (Figs. 3, 6 not shown) when the packings of the two may be mixed in a boundary zone or separated by a porous plate &c. and means are provided for supplying the reagent(s) laterally to the porous plate or to a point between the porous plate and the reaction tube. In the embodiment shown, jacketed reactor tube 1 has an inlet closure comprising elastic ring 9, seal body 8 and bolt 12 having a lateral channel 16 communicating with the inlet 10 and holding between its head and body an annular porous body 13 comprising layers of filter paper or porous polytetrafluoroethylene plates, and an outlet closure comprising porous disc 3 which may be of sintered glass, and polytetrafluoroethylene disc 4 having in its surface grooves communicating with outlet duct 5. The reaction mixture is optionally maintained at super-atmospheric pressure. There is reference to automatic analysis of amino acids using a cation exchange resin.