GB1173996A - Automatic Liquid Chromatography and Chromatograph - Google Patents

Automatic Liquid Chromatography and Chromatograph

Info

Publication number
GB1173996A
GB1173996A GB2180367A GB2180367A GB1173996A GB 1173996 A GB1173996 A GB 1173996A GB 2180367 A GB2180367 A GB 2180367A GB 2180367 A GB2180367 A GB 2180367A GB 1173996 A GB1173996 A GB 1173996A
Authority
GB
United Kingdom
Prior art keywords
colour
column
saturated
basic metal
weakly basic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2180367A
Inventor
Yoshijiro Arikawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to GB2180367A priority Critical patent/GB1173996A/en
Publication of GB1173996A publication Critical patent/GB1173996A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

1,173,996. Chromatography; ion exchange. HITACHI Ltd. 10 May, 1967, No. 21803/67. Headings B1J and B1X. Components of organic mixtures, especially amino acid mixtures, are separated chromatographically by means of a cation exchange resin which has been saturated with a weakly basic metal ion which readily complexes with the components of the mixture, the separated components being detected as the complexes so formed, suitably by means of a colour reaction. Column 1 comprises resin saturated with weakly basic metal ions such as any of Ni##, Cu##, Co##, Cd##, Hg##, Zn##, La### or Y###; the amino acid mixture is introduced through cap 18. Eluent from tank 3, suitably a buffered solution which contains the same weakly basic metal ion as that with which the column is saturated, is pumped by pump 2 into the column. The effluent is continuously mixed with a colour reagent from tank 9 in coil 10 in bath 11 (which may be heated), and the developed colour continuously read by photometer 6 and recorded on recorder 7. Suitable ion exchange resins are those containing sulphonic, carboxylic or iminodiacetic acid groups. In the separation of amino acids, suitable colour-developing reagents are 2-carboxy-2-hydroxy-5'-formazyl benzene, xylenol orange, pyrocatechol violet and ninhydrin.
GB2180367A 1967-05-10 1967-05-10 Automatic Liquid Chromatography and Chromatograph Expired GB1173996A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB2180367A GB1173996A (en) 1967-05-10 1967-05-10 Automatic Liquid Chromatography and Chromatograph

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2180367A GB1173996A (en) 1967-05-10 1967-05-10 Automatic Liquid Chromatography and Chromatograph

Publications (1)

Publication Number Publication Date
GB1173996A true GB1173996A (en) 1969-12-10

Family

ID=10169085

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2180367A Expired GB1173996A (en) 1967-05-10 1967-05-10 Automatic Liquid Chromatography and Chromatograph

Country Status (1)

Country Link
GB (1) GB1173996A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0202437A3 (en) * 1980-05-27 1987-04-01 The Dow Chemical Company Ion exchange chromatography with indirect photometric detection
EP0368092A1 (en) * 1988-10-28 1990-05-16 Anthony R. Torres Ion exchange and separation method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0202437A3 (en) * 1980-05-27 1987-04-01 The Dow Chemical Company Ion exchange chromatography with indirect photometric detection
EP0368092A1 (en) * 1988-10-28 1990-05-16 Anthony R. Torres Ion exchange and separation method

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Legal Events

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PCNP Patent ceased through non-payment of renewal fee