GB1120249A - A method of two- or multistage continuous biotransformation or biosynthesis - Google Patents
A method of two- or multistage continuous biotransformation or biosynthesisInfo
- Publication number
- GB1120249A GB1120249A GB34966/65A GB3496665A GB1120249A GB 1120249 A GB1120249 A GB 1120249A GB 34966/65 A GB34966/65 A GB 34966/65A GB 3496665 A GB3496665 A GB 3496665A GB 1120249 A GB1120249 A GB 1120249A
- Authority
- GB
- United Kingdom
- Prior art keywords
- stage
- micro
- rate
- growth
- azauracil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
In a multi-stage process for continuous cultivation of micro-organisms on assimilable carbon, nitrogen and inorganic salts within a liquid substrate with the production of biochemical products, e.g. glucose oxidase and amino acids, or ribonucleic acid proportionately with the growth of micro-organisms, at any stage between the first and penultimate stages partly fermented substrate containing the micro-organism culture in a phase of retarded growth is transferred to a following fermentation stage in which the culture exists in a phase of exponential growth, and this following stage is then fed with fresh substrate. An additive which will act in the continuous process to yield a product but is toxic to the microorganism, may be introduced at the penultimate fermentation stage. According to Example 2, Aspergillus niger was grown in sugar-beet molasses (pH 6.0) in two stages at dilution rates of 0.04-0.05 (retarded growth) and 0.12-0.14 (exponential growth) respectively, giving optimum production of glucose oxidase. In Example 3, Escherichia coli was cultivated in two stages upon specified media, each containing 6-azauracil, to obtain ribonucleic acid. In the first stage the rate of synthesis of the ribonucleic acid was considerably reduced due to the inhibitory effect of the 6-azauracil, whereby the rate of 6-azauracil riboside synthesis corresponded to the rate of the ribonucleic acid synthesis.ALSO:In a multi-stage process for continuous cultivation of micro-organisms on assimilable carbon, nitrogen and inorganic salts within a liquid substrate with the production of biochemical products (sorbose, glucose oxidase, ribonucleic acid, antibiotics, amino acids and other organic acids) proportionately with the growth of micro-organisms, at any stage between the first and penultimate stages partly fermented substrate containing the micro-organism culture in a phase of retarded growth is transferred to a following fermentation stage in which the culture exists in a phase of exponential growth, and this following stage is then fed with fresh substrate. An additive which will act in the continuous process to yield a product but is toxic to the micro-organism, may be introduced at the penultimate fermentation stage. According to the Examples: (1) a culture of Acetobacter suboxidans was grown in a state of retarded growth upon a sorbitol substrate, containing additionally corn steep liquor, yeast extract, disodium hydrogen phosphate and sodium carbonate, at a dilution rate of 0.1 - 0.11. The fermented medium together with the micro-organism culture was passed into a second fermentation stage for exponential growth of the micro-organism, to which stage fresh substrate was being fed so that the total dilution rate in this stage was 0.45. There followed a third stage with the same dilution rate. The yield of sorbose amounted to 98% of the sorbitol processed. (2) Aspergillus niger was grown on sugar-beet molasses (pH 6.0) in two stages at dilution rates of 0.04 - 0.05 (retarded growth) and 0.12 - 0.14 (exponential growth) respectively, giving optimum production of glucose oxidase. (3) Escherichia coli was cultivated in two stages upon specified media containing 6-azauracil in the second stage, to obtain ribonucleic acid and azauracil riboside. The rate of synthesis of the ribonucleic acid was considerably reduced due to the inhibitory effect of the 6-azauracil, whereby the rate of 6-azauracil riboside synthesis corresponded to the rate of ribonudeic acid synthesis.ALSO:In a multi-stage process for continuous cultivation of micro-organisms on assimilable carbon, nitrogen and inorganic salts within a liquid substrate with the production of biochemical products (sorbose, glucose oxidase, ribonucleic acid, antibiotics, amino acids and other organic acids) proportionately with the growth of micro-organisms, at any stage between the first and penultimate stages partly fermented substrate containing the micro-organism culture in a phase of retarded growth is transferred to a following fermentation stage in which the culture exists in a phase of exponential growth, and this following stage is then fed with fresh substrate. An additive which will act in the continuous process to yield a product but is toxic to the micro-organism, may be introduced at the penultimate fermentation stage. According to the examples: (1) a culture of Acetobacter suboxidans was grown in a state of retarded growth upon a sorbitol substrate, containing additionally corn steep liquor, yeast extract, disodium hydrogen phosphate and sodium carbonate, at a dilution rate of 0.1-0.11. The fermented medium together with the micro-organism culture was passed into a second fermentation stage for exponential growth of the micro-organism, to which stage fresh substrate was being fed so that the total dilution rate in this stage was 0.45. There followed a third stage with the same dilution rate. The yield of sorbose amounted to 98% of the sorbitol processed. (2) Aspergillus niger was grown on sugar-beet molasses (pH 6.0) in two stages at dilution rates of 0.04-0.05 (retarded growth) and 0.12-0.14 (exponential growth) respectively, giving optimum production of glucose oxidase. (3) Escherichia coli was cultivated in two stages upon specified media containing 6-azauracil in the second stage, to obtain ribonucleic acid and azauracil riboside. The rate of synthesis of the ribonucleic acid was considerably reduced due to the inhibitory effect of the 6-azauracil, whereby the rate of 6-azauracil riboside synthesis corresponded to the rate of ribonucleic acid synthesis.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CS526864 | 1964-09-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1120249A true GB1120249A (en) | 1968-07-17 |
Family
ID=5397237
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB34966/65A Expired GB1120249A (en) | 1964-09-22 | 1965-08-16 | A method of two- or multistage continuous biotransformation or biosynthesis |
Country Status (2)
Country | Link |
---|---|
DE (1) | DE1442227A1 (en) |
GB (1) | GB1120249A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4307334A1 (en) * | 1993-03-09 | 1994-09-15 | Chema Balcke Duerr Verfahrenst | Process and system for the production of inoculation material for improved crude oil production |
EP0972843A1 (en) * | 1998-07-17 | 2000-01-19 | F. Hoffmann-La Roche Ag | Continuous fermentation process |
US6238897B1 (en) | 1998-07-17 | 2001-05-29 | Roche Vitamins Inc. | Continuous process for producing 2-keto-L-gulonic acid |
-
1965
- 1965-08-11 DE DE19651442227 patent/DE1442227A1/en active Pending
- 1965-08-16 GB GB34966/65A patent/GB1120249A/en not_active Expired
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4307334A1 (en) * | 1993-03-09 | 1994-09-15 | Chema Balcke Duerr Verfahrenst | Process and system for the production of inoculation material for improved crude oil production |
EP0972843A1 (en) * | 1998-07-17 | 2000-01-19 | F. Hoffmann-La Roche Ag | Continuous fermentation process |
US6238897B1 (en) | 1998-07-17 | 2001-05-29 | Roche Vitamins Inc. | Continuous process for producing 2-keto-L-gulonic acid |
Also Published As
Publication number | Publication date |
---|---|
DE1442227A1 (en) | 1969-01-23 |
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