GB1076087A - A method of producing proteins by a continuous fermentation of hydrocarbons - Google Patents

A method of producing proteins by a continuous fermentation of hydrocarbons

Info

Publication number
GB1076087A
GB1076087A GB5318565A GB5318565A GB1076087A GB 1076087 A GB1076087 A GB 1076087A GB 5318565 A GB5318565 A GB 5318565A GB 5318565 A GB5318565 A GB 5318565A GB 1076087 A GB1076087 A GB 1076087A
Authority
GB
United Kingdom
Prior art keywords
aqueous phase
fermented
hydrocarbons
plasmolysed
yeast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5318565A
Inventor
Zdenek Fencl
Vladimir Munk
Milan Dostalek
Milan Rosa
Jana Vernerova
Vladimir Sillenger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Czech Academy of Sciences CAS
Original Assignee
Czech Academy of Sciences CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Czech Academy of Sciences CAS filed Critical Czech Academy of Sciences CAS
Publication of GB1076087A publication Critical patent/GB1076087A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C12N1/28—Processes using, or culture media containing, hydrocarbons aliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

In the continuous microbiological production of proteins, an aqeous substrate containing straight chain hydrocarbons and inorganic nutrient substances is continuously fermented under aerobic conditions with micro-organisms capable of growth upon straight chain hydrocarbons, a fermented hydrocarbon phase is separated from the aqueous phase as by centrifuging or flotation, the aqueous phase is subjected to plasmolysis and the plasmolysed aqueous phase is returned to the mass which is being continuously fermented. Aqueous phase separated from the fermented hydrocarbon phase under such conditions that it still contains 0.05-0.1 g/l of yeast, may be plasmolysed by heating to a temperature of 45-80 DEG C. for 2-4 hours, sufficient to autolyse the yeast, or by adjusting the pH to 7.5-8.5 by addition of ammonia and then heating.
GB5318565A 1964-12-23 1965-12-15 A method of producing proteins by a continuous fermentation of hydrocarbons Expired GB1076087A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CS732164A CS161198B1 (en) 1964-12-23 1964-12-23

Publications (1)

Publication Number Publication Date
GB1076087A true GB1076087A (en) 1967-07-19

Family

ID=5422121

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5318565A Expired GB1076087A (en) 1964-12-23 1965-12-15 A method of producing proteins by a continuous fermentation of hydrocarbons

Country Status (6)

Country Link
JP (1) JPS5130152B1 (en)
CH (1) CH485850A (en)
CS (1) CS161198B1 (en)
DE (1) DE1442228A1 (en)
FR (1) FR1461228A (en)
GB (1) GB1076087A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS53155555U (en) * 1977-05-10 1978-12-06

Also Published As

Publication number Publication date
JPS5130152B1 (en) 1976-08-30
CS161198B1 (en) 1975-06-10
DE1442228A1 (en) 1969-10-30
CH485850A (en) 1970-02-15
FR1461228A (en) 1966-12-10

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