GB1056292A - Improvements in or relating to insecticidal cultures of micro-organisms - Google Patents

Improvements in or relating to insecticidal cultures of micro-organisms

Info

Publication number
GB1056292A
GB1056292A GB8669/64A GB866964A GB1056292A GB 1056292 A GB1056292 A GB 1056292A GB 8669/64 A GB8669/64 A GB 8669/64A GB 866964 A GB866964 A GB 866964A GB 1056292 A GB1056292 A GB 1056292A
Authority
GB
United Kingdom
Prior art keywords
dust
spores
kaolin
galleriae
bacillus cereus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB8669/64A
Inventor
Olgaivanovna Shvetzova
Max Grigorevich Gandman
Engelina Robertovna Zurabova
Nadezhoa Pavlovna Isakova
Anastasia Iakovlevna Leskova
Nickolai Stepan Ov Fedorinchik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VSESOIUZNY NI SKY I ZASHCHITY
Original Assignee
VSESOIUZNY NI SKY I ZASHCHITY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VSESOIUZNY NI SKY I ZASHCHITY filed Critical VSESOIUZNY NI SKY I ZASHCHITY
Priority to GB8669/64A priority Critical patent/GB1056292A/en
Publication of GB1056292A publication Critical patent/GB1056292A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/085—Bacillus cereus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Bacillus cereus var. galleriae, morphological and cultural characteristics of which are disclosed, is cultivated on an initially sterile liquid medium of pH 6.6 - 7.2 containing specified proportions of maize extract, molasses, glucose or pea hydrolysate, and sunflower oil or spermaceti fat as an anti-foaming agent. On a large scale the fermentation is conducted with intermittent stirring in vats held at a temperature of 30 DEG -31 DEG C. for between 36 and 48 hours, and 0.8-1.0 litre of air is blown through the medium in the vats per minute for each litre of medium. The fermentation is stopped when 90-95% of the cells of the organism have produced spores and the spores have escaped from the cells, and 0.5% of kaolin is then added to the liquid medium. The whole culture is stirred and the kaolin with the spores is separated as a paste which is freeze-dried to 3% moisture content. The freeze-dried material is ground to dust in a ball mill. The dust, further diluted with kaolin or chalk and containing 3 X 109 spores per gram, is applied as an insecticide to flower or vegetable crops.ALSO:An insecticidal dust consists of spores of Bacillus cereus var galleriae (3x109 per gram of dust) and kaolin or chalk. It may be applied, preferably at a temperature of at least 20 DEG C., to flower crops (3-5 Kg. dust per hectare) and vegetable crops (1-3 Kg. dust per hectare), both during the blossoming period and shortly before harvest. In order to increase the efficiency of kill at low temperatures, 0.002-0.005 of DDT or hexachloro-cyclohexane may be included in the dust. Alternatively the spores of Bacillus cereus var. galleriae can be applied directly by spraying.
GB8669/64A 1964-03-02 1964-03-02 Improvements in or relating to insecticidal cultures of micro-organisms Expired GB1056292A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB8669/64A GB1056292A (en) 1964-03-02 1964-03-02 Improvements in or relating to insecticidal cultures of micro-organisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8669/64A GB1056292A (en) 1964-03-02 1964-03-02 Improvements in or relating to insecticidal cultures of micro-organisms

Publications (1)

Publication Number Publication Date
GB1056292A true GB1056292A (en) 1967-01-25

Family

ID=9856948

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8669/64A Expired GB1056292A (en) 1964-03-02 1964-03-02 Improvements in or relating to insecticidal cultures of micro-organisms

Country Status (1)

Country Link
GB (1) GB1056292A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10986842B2 (en) 2015-11-10 2021-04-27 Chr. Hansen A/S Microbial pesticidal composition and production thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10986842B2 (en) 2015-11-10 2021-04-27 Chr. Hansen A/S Microbial pesticidal composition and production thereof

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