GB1021401A - Novel antibiotics and processes for their manufacture - Google Patents
Novel antibiotics and processes for their manufactureInfo
- Publication number
- GB1021401A GB1021401A GB39130/62A GB3913062A GB1021401A GB 1021401 A GB1021401 A GB 1021401A GB 39130/62 A GB39130/62 A GB 39130/62A GB 3913062 A GB3913062 A GB 3913062A GB 1021401 A GB1021401 A GB 1021401A
- Authority
- GB
- United Kingdom
- Prior art keywords
- gentamycin
- nrrl
- echinospora
- produced
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/22—Cyclohexane rings, substituted by nitrogen atoms
- C07H15/222—Cyclohexane rings substituted by at least two nitrogen atoms
- C07H15/226—Cyclohexane rings substituted by at least two nitrogen atoms with at least two saccharide radicals directly attached to the cyclohexane rings
- C07H15/234—Cyclohexane rings substituted by at least two nitrogen atoms with at least two saccharide radicals directly attached to the cyclohexane rings attached to non-adjacent ring carbon atoms of the cyclohexane rings, e.g. kanamycins, tobramycin, nebramycin, gentamicin A2
- C07H15/236—Cyclohexane rings substituted by at least two nitrogen atoms with at least two saccharide radicals directly attached to the cyclohexane rings attached to non-adjacent ring carbon atoms of the cyclohexane rings, e.g. kanamycins, tobramycin, nebramycin, gentamicin A2 a saccharide radical being substituted by an alkylamino radical in position 3 and by two substituents different from hydrogen in position 4, e.g. gentamicin complex, sisomicin, verdamycin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
- C12P19/48—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin the cyclohexyl radical being substituted by two or more nitrogen atoms, e.g. destomycin, neamin
- C12P19/485—Having two saccharide radicals bound through only oxygen to non-adjacent ring carbons of the cyclohexyl radical, e.g. gentamycin, kanamycin, sisomycin, verdamycin, mutamycin, tobramycin, nebramycin, antibiotics 66-40B, 66-40D, XK-62-2, 66-40, G-418, G-52
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/29—Micromonospora
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Antibiotics gentamycin and/or BA-3 (fractions A and B) may be produced by incubating a microorganism of the species Micromonospora purpurea or Micromonospore echinospora, or a gentamycin and/or BA-3 producing mutant or variant thereof, until at least one compound having substantial antibiotic activity is produced and isolating at least one of the antibiotic compounds thus produced. The microorganism may be M. purpeyea NRRL 2953, M. echinospora NRRL 2985, Mechinospora var. ferruginea NRRL 2995 or M. echinospora var. pallida NRRL 2996. The micro-organism may be incubated under submerged aerobic conditions at a temperature from 25 DEG to 40 DEG C. and a pH from 6 to 8 in an aqueous nutrient medium containing assimilable carbon and nitrogen and, after incubation, the mycelium is extracted with acid and the antibiotic recovered from the acid extract by adsorption or fractionate salt precipitation or a combination of both methods. Gentamycin is a basic organic substance having a melting point of 102 DEG to 108 DEG .C with decomposition, a specific optical rotation [a ]25D = + 146 degrees (1% in water), an elementary analysis C = 50.40%, H = 8.52%, N = 13.47%, 0 = 27.81% and a M.W. of 543; it is very soluble in water, aqueous acid media and methanol and substantially insoluble in ether, benzene and halogenated hydrocarbons; it is transparent to U.V. radiation in the range 220 to 400 mm . The Specification gives details of its I.R. spectrum and antibacterial spectrum, and also refers to salts of gentamycin with acids, reaction products with bisulphite addition compounds of aldehydes and ketones, N- and O-acyl derivatives of gentamycin and Schiff's bases derived therefrom. BA-3 contains C, H, N and O, with no other elements present and has solubility characteristics similar to gentamycin; it is transparent to U.V. radiation in the range 220 to 400 mm and forms a sulphate which is a white amorphous powder, soluble in water and insoluble in ethanol, methanol and acetone. BA-3 may be resolved into fractions A and B by paper chromatography.ALSO:Pharmaceutical compositions suitable for use for injections, as topical ointments and as ophthalmic drops contain gentamycin sulphate with usual pharmaceutical carriers. The ophthalmic drops may also contain phenyl-ethyl alcohol.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15226261A | 1961-11-14 | 1961-11-14 | |
| US19841462A | 1962-05-23 | 1962-05-23 | |
| US211153A US3091572A (en) | 1962-07-16 | 1962-07-16 | Gentamycin and method of production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1021401A true GB1021401A (en) | 1966-03-02 |
Family
ID=27387225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB39130/62A Expired GB1021401A (en) | 1961-11-14 | 1962-10-16 | Novel antibiotics and processes for their manufacture |
Country Status (9)
| Country | Link |
|---|---|
| AT (1) | AT246917B (en) |
| BE (1) | BE624816A (en) |
| CA (2) | CA922250A (en) |
| CH (1) | CH428098A (en) |
| CY (1) | CY357A (en) |
| DE (1) | DE1218660B (en) |
| DK (1) | DK120770B (en) |
| GB (1) | GB1021401A (en) |
| NO (1) | NO118368B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR128F (en) * | 1962-04-02 |
-
1962
- 1962-10-16 GB GB39130/62A patent/GB1021401A/en not_active Expired
- 1962-10-23 NO NO62146184A patent/NO118368B/no unknown
- 1962-11-07 CH CH1309762A patent/CH428098A/en unknown
- 1962-11-12 AT AT886862A patent/AT246917B/en active
- 1962-11-13 DK DK487662AA patent/DK120770B/en unknown
- 1962-11-13 DE DESCH35791A patent/DE1218660B/en active Pending
- 1962-11-14 BE BE624816D patent/BE624816A/fr unknown
-
1966
- 1966-01-21 CA CA950460A patent/CA922250A/en not_active Expired
- 1966-12-30 CY CY35766A patent/CY357A/en unknown
-
1971
- 1971-08-17 CA CA120789A patent/CA933153A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| BE624816A (en) | 1963-05-14 |
| AT246917B (en) | 1966-05-10 |
| DK120770B (en) | 1971-07-12 |
| CH428098A (en) | 1967-01-15 |
| CA933153A (en) | 1973-09-04 |
| DE1218660B (en) | 1966-06-08 |
| CY357A (en) | 1966-12-30 |
| CA922250A (en) | 1973-03-06 |
| NO118368B (en) | 1969-12-22 |
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