FR3110246B1 - procédé de détection d’une forme agrégée en feuillets β d’une protéine formant des agrégats de type feuillets β - Google Patents

procédé de détection d’une forme agrégée en feuillets β d’une protéine formant des agrégats de type feuillets β Download PDF

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Publication number
FR3110246B1
FR3110246B1 FR2004948A FR2004948A FR3110246B1 FR 3110246 B1 FR3110246 B1 FR 3110246B1 FR 2004948 A FR2004948 A FR 2004948A FR 2004948 A FR2004948 A FR 2004948A FR 3110246 B1 FR3110246 B1 FR 3110246B1
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FR
France
Prior art keywords
sheet
detecting
aggregates
protein
aggregated form
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Active
Application number
FR2004948A
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English (en)
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FR3110246A1 (fr
Inventor
Eric J Trinquet
Alexis G Aquilina
Maud E Pratlong
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Cisbio Bioassays SAS
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Cisbio Bioassays SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority to FR2004948A priority Critical patent/FR3110246B1/fr
Application filed by Cisbio Bioassays SAS filed Critical Cisbio Bioassays SAS
Priority to CN202180060491.7A priority patent/CN116194780A/zh
Priority to US17/925,981 priority patent/US20230314450A1/en
Priority to JP2022570442A priority patent/JP2023528267A/ja
Priority to EP21732466.4A priority patent/EP4153998A1/fr
Priority to PCT/FR2021/050854 priority patent/WO2021234261A1/fr
Priority to CA3179103A priority patent/CA3179103A1/fr
Publication of FR3110246A1 publication Critical patent/FR3110246A1/fr
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Publication of FR3110246B1 publication Critical patent/FR3110246B1/fr
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé de détection d’une forme agrégée en feuillets β d’une protéine formant des agrégats de type feuillets β L’invention concerne un procédé in vitro de détection dans un échantillon d’une forme agrégée en feuillets β d’une protéine formant des agrégats de type feuillets β (PAFβ) comprenant une étape qui consiste à ajuster le pH d’un échantillon susceptible de contenir une PAFβ à un pH allant de 9,7 à 13,2 pour désagréger tout ou partie de la PAFβ afin d’obtenir une forme non-agrégée en feuillets β de la protéine formant des agrégats de type feuillets β (PNAFβ) et la mesure de la teneur en PNAFβ avec une méthode immunologique appropriée à un pH allant de 6 à 9. FIGURES
FR2004948A 2020-05-18 2020-05-18 procédé de détection d’une forme agrégée en feuillets β d’une protéine formant des agrégats de type feuillets β Active FR3110246B1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
FR2004948A FR3110246B1 (fr) 2020-05-18 2020-05-18 procédé de détection d’une forme agrégée en feuillets β d’une protéine formant des agrégats de type feuillets β
US17/925,981 US20230314450A1 (en) 2020-05-18 2021-05-17 METHOD FOR DETECTING A ß-SHEET AGGREGATE FORM OF A PROTEIN FORMING ß-SHEET AGGREGATES
JP2022570442A JP2023528267A (ja) 2020-05-18 2021-05-17 βシート凝集体を形成するタンパク質のβシート凝集体形態を検出する方法
EP21732466.4A EP4153998A1 (fr) 2020-05-18 2021-05-17 Procede de detection d'une forme agregee en feuillets beta d'une proteine formant des agregats de type feuillets beta
CN202180060491.7A CN116194780A (zh) 2020-05-18 2021-05-17 检测形成β-片层聚集体的蛋白质的β-片层聚集体形式的方法
PCT/FR2021/050854 WO2021234261A1 (fr) 2020-05-18 2021-05-17 PROCEDE DE DETECTION D'UNE FORME AGREGEE EN FEUILLETS Bβ D'UNE PROTEINE FORMANT DES AGREGATS DE TYPE FEUILLETS Bβ
CA3179103A CA3179103A1 (fr) 2020-05-18 2021-05-17 Procede de detection d'une forme agregee en feuillet beta d'une proteine formant des agregats de type feuillet beta

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2004948 2020-05-18
FR2004948A FR3110246B1 (fr) 2020-05-18 2020-05-18 procédé de détection d’une forme agrégée en feuillets β d’une protéine formant des agrégats de type feuillets β

Publications (2)

Publication Number Publication Date
FR3110246A1 FR3110246A1 (fr) 2021-11-19
FR3110246B1 true FR3110246B1 (fr) 2022-06-03

Family

ID=72560697

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2004948A Active FR3110246B1 (fr) 2020-05-18 2020-05-18 procédé de détection d’une forme agrégée en feuillets β d’une protéine formant des agrégats de type feuillets β

Country Status (7)

Country Link
US (1) US20230314450A1 (fr)
EP (1) EP4153998A1 (fr)
JP (1) JP2023528267A (fr)
CN (1) CN116194780A (fr)
CA (1) CA3179103A1 (fr)
FR (1) FR3110246B1 (fr)
WO (1) WO2021234261A1 (fr)

Also Published As

Publication number Publication date
JP2023528267A (ja) 2023-07-04
WO2021234261A8 (fr) 2022-11-24
CN116194780A (zh) 2023-05-30
EP4153998A1 (fr) 2023-03-29
FR3110246A1 (fr) 2021-11-19
CA3179103A1 (fr) 2021-11-25
US20230314450A1 (en) 2023-10-05
WO2021234261A1 (fr) 2021-11-25

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