FR3070108B1 - ANTIMICROBIAL MIXTURE CONTAINING 4- (3-ETHOXY-4-HYDROXYPHENYL) BUTAN-2-ONE AND A POLYHEXAMETHYLENE BIGUANIDE SALT, AND A COSMETIC COMPOSITION CONTAINING THE SAME - Google Patents

Abstract

The invention relates to an antimicrobial mixture containing 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one and a polyhexamethylene biguanide salt, as well as a cosmetic composition containing such a mixture. Application to the care, makeup and cleansing of keratin materials.

Description

The subject of the present invention is an antimicrobial mixture containing 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one and a polyhexamethylene biguanide salt, as well as a cosmetic composition containing such a mixture.

4- (3-Ethoxy-4-hydroxyphenyl) butan-2-one (ketone compound) is a substance of interest as a preservative of cosmetic compositions for protecting the compositions from microbial contamination, as described in WO 2011/039445 .

However, it is desirable to incorporate said ketone compound in reduced concentration in compositions, especially cosmetic or dermatological, while maintaining a good antimicrobial preservation performance. It is therefore sought for this purpose combinations of the ketone compound with other compounds having antimicrobial efficacy.

The inventors have discovered, unexpectedly, that the combination of 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one with a polyhexamethylene biguanide salt, in particular the hydrochloride salt, makes it possible to obtain a Antimicrobial mixture with a synergistic antimicrobial activity, especially on molds, especially on Aspergillus niger.

The results of Example 1 described hereinafter show the synergistic antimicrobial activity obtained with the minimum inhibitory concentration (MIC) measurements made with several mixtures. The antimicrobial activity is considered to be synergistic when the antimicrobial mixture makes it possible to obtain a percentage of growth of the strain of less than or equal to 20%.

Applications FR-A-2962328 and FR-A-2973229 describe a cosmetic composition for treating body odors related to human perspiration comprising a 2-alkoxy-4-alkylketonephenol compound, and optionally an additional deodorant active agent such as polyhexamethylene salts. biguanide.

These documents do not specifically describe an antimicrobial mixture consisting of the combination of 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one with a polyhexamethylene biguanide salt, nor do they suggest that such a mixture is present. synergistic antimicrobial activity on molds, especially on Aspergillus niger.

More specifically, the subject of the invention is an antimicrobial mixture comprising or consisting of (or consisting of) 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one and a polyhexamethylene biguanide salt. The subject of the invention is also a composition, in particular a cosmetic composition, comprising in a physiologically acceptable medium the said mixture described above. The subject of the invention is also a non-therapeutic cosmetic treatment process for keratinous materials comprising the application to the keratin materials of a composition as described above. The process may be a cosmetic process for caring for or making up or cleaning keratin materials. The invention also relates to a method for preserving a composition comprising a physiologically acceptable medium, in particular a cosmetic or dermatological composition, characterized in that it consists in incorporating into said composition an antimicrobial mixture as described above.

4- (3-Ethoxy-4-hydroxyphenyl) butan-2-one is a compound of formula

The polyhexamethylene biguanide salt may be a salt selected from hydrochloride, borate, acetate, gluconate, sulphonate, tartrate, citrate, and in particular hydrochloride.

Preferably, polyhexamethylene biguanide hydrochloride (CTFA name: polyaminopropyl biguanide), such as that sold under the name COSMOCIL® CQ by Lonza (20% aqueous solution of compound), is used.

Advantageously, the polyhexamethylene biguanide salt has a weight average molecular weight (Mw) ranging from 1000 to 50000, preferably from 1000 to 20000, preferably ranging from 1000 to 10000, and more preferably ranging from 1000 to 5000.

Advantageously, the 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one and the polyhexamethylene biguanide salt are present in said mixture in a content such that the weight ratio 4- (3-ethoxy-4-hydroxyphenyl) butan -2-one / polyhexamethylene biguanide salt is from 5 to 75, preferably from 15 to 60, preferably from 20 to 55.

The antimicrobial mixture according to the invention has a synergy of antimicrobial activity, in particular on molds, in particular on Aspergillus niger.

According to a preferred embodiment of the invention, the polyhexamethylene biguanide salt present in the antimicrobial mixture is polyhexamethylene biguanide hydrochloride.

The invention also relates to a composition comprising, in a physiologically acceptable medium, the antimicrobial mixture described above.

Physiologically acceptable medium means a medium compatible with keratin materials of human beings such as the skin, the scalp, the hair, the nails. The said medium may comprise one or more additional ingredients, distinct from the ketone compound and said polyhexamethylene biguanide salt.

The compound 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one may be present in the composition according to the invention, in a content ranging from 0.01% to 5% by weight, relative to the total weight of the composition, preferably ranging from 0.01% to 3% by weight, preferably ranging from 0.01 to 2.5% by weight, and more preferably ranging from 0.01 to 2% by weight.

The composition may comprise at least one additional ingredient selected from water, oils, polyols having 2 to 10 carbon atoms, gelling agents, surfactants, film-forming polymers, dyestuffs, fragrances, fillers, UV filters, plant extracts, cosmetic and dermatological active ingredients, and salts.

The composition according to the invention may comprise an aqueous phase.

The composition may comprise water which may be present in a content ranging from 5% to 90% by weight, based on the total weight of the composition, and preferably ranging from 35% to 75% by weight.

The composition may also comprise a water-miscible polyol at ambient temperature (25 ° C.), chosen in particular from polyols having in particular from 2 to 10 carbon atoms, preferably having from 2 to 6 carbon atoms, such as glycerine, propylene glycol, 1,3-propanediol, butylene glycol, pentylene glycol, hexylene glycol, dipropylene glycol, diethylene glycol, diglycerine.

The compositions according to the invention may be in the form of oil-in-water (O / W) or water-in-oil (W / O) or multiple (triple: W / O / E or H / E / H), oily solutions, oily gels, aqueous solutions, aqueous gels, solid compositions. These compositions are prepared according to the usual methods.

The compositions according to the invention can be more or less fluid and have the appearance of a white or colored cream, an ointment, a milk, a lotion, a serum, a paste, of a foam. They may optionally be applied to the skin in the form of an aerosol. They may also be in solid form, for example in the form of a stick or a compact powder.

The composition according to the invention may especially be in the form of: a makeup product, in particular the skin of the face, of the body or of the lips or eyelashes; - an aftershave gel or lotion; shaving product; - a deodorant (stick, roll-on, aerosol) - a depilatory cream; in the form of a personal hygiene composition such as a shower gel or a shampoo; a pharmaceutical composition; a solid composition such as a soap or a cleaning roll; an aerosol composition also comprising a propellant under pressure; - a styling lotion, a styling cream or gel, a dyeing composition, a permanent composition, a lotion or a fall-prevention gel, an after-treatment shampoo; - a care composition or cleansing of the skin. The subject of the invention is also a process for the preparation of a composition, in particular a cosmetic or dermatological composition, comprising a step of mixing 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one of said polyhexamethylene biguanide salt, and one or more additional ingredients, especially cosmetic or dermatological, such as those described above. The invention is further illustrated in the following example. The contents of the ingredients are expressed in percentage by weight.

Example 1: Determination of the synergistic antimicrobial activity in MIC

Demonstration of a synergistic effect of antimicrobial activity with a mixture of 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one (referred to as substance A) and polyhexamethylene biguanide hydrochloride (referred to as substance B) is achieved by calculating the synergy index (or FIC index) according to the following formula: FIC Index = (CMIa with B / CMIa) + (CMIb with A / CMIb) with: CMIa with B: minimum concentration of product A in l A + B combination to obtain a CMIb inhibitory effect with A: minimum concentration of product B in the association A + B to obtain the inhibitory effect. CMIa: minimal inhibitory concentration of product A alone. MICb: minimal inhibitory concentration of product B alone.

This formula has been described for the first time in the article by F. C. Kull, P. C. Eisman, H. D. Sylwestrowka, and R. L. Mayer, Applied Microbiology 9: 538-541, 1961.

For each compound tested alone, the MIC is considered the first concentration to obtain a percentage of microbial growth of less than or equal to 20%.

Regarding the associations tested, CMIa with b and CMIb with a are the respective concentrations of A and B in the combinations allowing to obtain a percentage of microbial growth less than or equal to 20%.

Interpretation of the FIC Index:

When the value of the index FIC is less than or equal to 1, it is considered that the combination of the tested compounds has a synergistic effect.

The summary of the results obtained is presented in the following tables. The combination of compounds A and B was tested on the Aspergillus niger strain.

The microbial strain Aspergillus niger ATCC 6275 was used, and Sabouraud broth liquid culture medium supplemented with polyoxyethylenesorbitan (20 EO) monopalmitate (Tween 40 from Croda) and Phytagel® BioReagent, at double concentration.

A 96-well microplate is used at an incubation temperature of 32.5 ° C. The incubation time of the microplate is 24 to 48 hours aerobically.

testing

For each compound: A = 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one compound B = polyhexamethylene biguanide hydrochloride compound (in aqueous solution at 20% active ingredient) sold under the name COSMOCIL® CQ by the company Lonza

A 10% (w / v) stock solution was prepared by mixing 1 g of compound in 9 ml of 1% aqueous agar solution. successive dilutions were made with the 1% o agar solution. Tests of compounds A and B alone 50 μl of each of the daughter solutions obtained containing compound A or B is added to the wells of the microplate. 100 μl of Sabouraud liquid nutrient broth inoculated with the Aspergillus niger strain and 50 μl of aqueous 1% agar solution are also added. • Tests of compounds A + B in mixture 50 μl of each of the daughter solutions obtained containing compound A and 50 μl of each of the daughter solutions obtained containing compound B are added to the wells of the microplate. It also adds 100μΙ of liquid nutrient broth Sabouraud seeded double concentration by the strain Aspergillus niger. Microbial growth control

A positive control of microbial growth has also been realized. The positive microbial growth control corresponds to the mixture of 100 μΙ of a 1% aqueous solution of agar with 100 μl of Sabouraud liquid nutrient broth inoculated at double concentration by the Aspergillus niger strain in the absence of the compounds A and B. absorbance of compounds A and B alone

In parallel, an absorbance control of compounds A and B alone was carried out. This control corresponds to 100μΙ of sterile Sabouraud liquid nutrient broth with double concentration + 100μΙ of compound A or B at double concentration.

In all three cases (absorbance control, growth control and test), the final volume present in each of the wells of the microplate is 200 μ -1.

In both cases (test and control), the inoculum represents the concentration of the Aspergillus niger strain present in the final volume of the wells (200 μΙ) and is between 2 and 6 × 10 5 cfu / ml in Aspergillus niger.

The minimum inhibitory concentration (MIC) of each compound A and B alone and in combination was determined in a known manner using optical density measurements at 620 nm wavelength.

The following results were obtained: (mass percentage of active ingredient)

Aspergillus niger

The results obtained show a synergy of the inhibitory activity for the mixtures: i) 0.0625% of A and 0.0025% of B is ratio A / B = 25 ii) 0.125% of A and 0.0025% of B A / B ratio = 50 iii) 0.0625% of A and 0.005% of B is ratio A / B = 12.5 iv) 0.125% of A and 0.005% of B is ratio A / B = 25 v) 0 , 0625% of A and 0.01% of B is ratio A / B = 6.25 vi) 0.125% of A and 0.01% of B is ratio A / B = 12.5

Claims (3)

An antimicrobial mixture comprising 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one and a polyhexamethylene biguanide salt. 2. Mixture according to the preceding claim, characterized in that it comprises 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one and a polyhexamethylene biguanide salt in amounts such that the weight ratio 4- (3- ethoxy-4-hydroxyphenyl) butan-2-one / polyhexamethylene biguanide salt is from 5 to 75, preferably from 15 to 60, preferably from 20 to 55. 3. An antimicrobial mixture according to one of the preceding claims, characterized in that it exhibits antimicrobial activity on the mold Aspergillus niger. 4. Antimicrobial mixture according to one of the preceding claims, characterized in that the polyhexamethylene biguanide salt is selected from hydrochloride, borate, acetate, gluconate, sulfonate, tartrate, citrate. 5. antimicrobial mixture according to one of the preceding claims, characterized in that the polyhexamethylene biguanide salt is the hydrochloride salt. 6. Composition comprising, in a physiologically acceptable medium, an antimicrobial mixture according to one of claims 1 to 5. 7. Composition according to the preceding claim, characterized in that it comprises at least one additional ingredient selected from water, oils, polyols having 2 to 10 carbon atoms, gelling agents, surfactants, film-forming polymers, dyestuffs, perfumes, fillers, UV filters, plant extracts, cosmetic and dermatological active agents, and salts. 8. Composition according to one of claims 6 or 7, characterized in that the 4- (3-ethoxy-4-hydroxyphenyl) butan-2-one is present in a content ranging from 0.01% to 5% by weight , relative to the total weight of the composition, preferably ranging from 0.01% to 3% by weight, and preferably ranging from 0.01 to 2.5% by weight. 9. A method of non-therapeutic cosmetic treatment of care and / or makeup and / or cleaning of keratin materials comprising the application on said keratin materials of a composition according to any one of claims 6 to 8. 10. Process of conservation of a composition comprising a physiologically acceptable medium, in particular a cosmetic or dermatological composition, characterized in that it consists in incorporating into said composition an antimicrobial mixture as defined according to one of claims 1 to 5. No. national registration: FR1757742 Publication no .: FR3070108 RESEARCH REPORT articles L.612-14, L.612-53 to 69 of the Intellectual Property Code SUBJECT OF THE RESEARCH REPORT The INPI appendix to each patent a "RESEARCH REPORT" citing the elements of the state of the art that may be taken into account to assess the patentability of the invention, within the meaning of Articles L. 611-11 (novelty) and L. 611-14 (inventive step) of the intellectual property code. This report deals with the claims of the patent which define the object of the invention and delimit the scope of protection. After delivery, ΓΙ.Ν.Ρ.Ι. may, at the request of any interested person, make a "DOCUMENTARY ADVICE" on the basis of the documents cited in this search report and any other document that the applicant wishes to be taken into consideration. CONDITIONS FOR ESTABLISHING THIS RESEARCH REPORT [x] The applicant submitted comments in response to the preliminary search report. [x] The plaintiff maintained the claims. □ The applicant amended the claims. □ The applicant amended the description to remove items that were no longer consistent with the new claims. □ Third parties submitted comments after publication of the preliminary search report. □ A complementary preliminary research report has been prepared. DOCUMENTS CITED IN THIS RESEARCH REPORT The distribution of documents between headings 1, 2 and 3 takes into account, where appropriate, the claims filed last and / or the observations submitted. [x] The documents listed in section 1 below may be taken into consideration in assessing the patentability of the invention. □ The documents listed in Section 2 below illustrate the general background technology. □ The documents listed in section 3 below were cited during the procedure, but their relevance depends on the validity of the claimed priorities. □ No documents were cited during the procedure. National Registration No .: FR1757742 Publication No .: FR3070108 ΪΓ SELECTED TELECENT ELEMENTS BEFORE INJURY IN CONSIDERATION TO ASSESS PATENTABILITY OF INVENTION _______ | US 2015/265666 A1 (MODAK SHANTA M [US] AND AL ) September 24, 2015 (2015-09-24) | WO 2011/039445 A1 (OREAL [EN]; DALKO MARI A [EN]) April 7, 2011 (2011 -04-07) 2. ELEMENTS OF THE STATE OF THE ART ILLUSTRATING THE GENERAL TECHNICAL BACKGROUND Ineant 3. ELEMENTS OF THE STATE OF THE TECHNIQUE WHERE THE RELEVANCE DEPENDS __________________________ ON THE VALIDITY OF PRIORITIES ___________________ Ineant 2/2