FR2955333B1 - Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux; - Google Patents

Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux;

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Publication number
FR2955333B1
FR2955333B1 FR1000152A FR1000152A FR2955333B1 FR 2955333 B1 FR2955333 B1 FR 2955333B1 FR 1000152 A FR1000152 A FR 1000152A FR 1000152 A FR1000152 A FR 1000152A FR 2955333 B1 FR2955333 B1 FR 2955333B1
Authority
FR
France
Prior art keywords
matrix
collagen
glycosaminoglycans
support
cell culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR1000152A
Other languages
English (en)
Other versions
FR2955333A1 (fr
Inventor
Dominique Vacher
Florence Heraud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ibsa Pharma Sas Fr
Original Assignee
Laboratoires Genevrier SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laboratoires Genevrier SAS filed Critical Laboratoires Genevrier SAS
Priority to FR1000152A priority Critical patent/FR2955333B1/fr
Priority to FR1150365A priority patent/FR2955334B1/fr
Publication of FR2955333A1 publication Critical patent/FR2955333A1/fr
Application granted granted Critical
Publication of FR2955333B1 publication Critical patent/FR2955333B1/fr
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)

Abstract

L'invention concerne l'utilisation d'une matrice de collagène réticulée poreuse, principalement composée de collagène de type II et de glycosaminoglycanes, comme support à la culture cellulaire in vitro de chondrocytes destinée à être implantée pour réparer les défauts localisés du cartilage et douée d'une stabilité mécanique et dimensionnelle en culture. Elle est composée de collagène de type II et de glycosaminoglycanes. Elle est réticulée chimiquement. Le procédé de préparation de la matrice comprend une étape de mélange du collagène II et du glycosaminoglycanes, une étape de lyophilisation, une étape de réticulation chimique et une étape de stérilisation ; la réticulation chimique de la composition de collagène II et de glycosaminoglycanes est effectuée à l'aide de N-hydroxysuccinimide (NHS) et d'éthyl-3(3-diméthylaminopropyl) carbodiimide (EDC). Cette matrice sert à la culture de chondrocytes autologues et plus particulièrement à la conservation des chondrocytes dans les pores de la matrice grâce à la taille et à la stabilité de son maillage.
FR1000152A 2010-01-15 2010-01-15 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux; Expired - Fee Related FR2955333B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR1000152A FR2955333B1 (fr) 2010-01-15 2010-01-15 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux;
FR1150365A FR2955334B1 (fr) 2010-01-15 2011-01-17 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1000152A FR2955333B1 (fr) 2010-01-15 2010-01-15 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux;

Publications (2)

Publication Number Publication Date
FR2955333A1 FR2955333A1 (fr) 2011-07-22
FR2955333B1 true FR2955333B1 (fr) 2014-03-21

Family

ID=42340603

Family Applications (2)

Application Number Title Priority Date Filing Date
FR1000152A Expired - Fee Related FR2955333B1 (fr) 2010-01-15 2010-01-15 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux;
FR1150365A Active FR2955334B1 (fr) 2010-01-15 2011-01-17 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux

Family Applications After (1)

Application Number Title Priority Date Filing Date
FR1150365A Active FR2955334B1 (fr) 2010-01-15 2011-01-17 Utilisation d'une matrice de collagene ii reticulee poreuse comme support a la culture de cellules et la regeneration d'un tissu cartilagineux

Country Status (1)

Country Link
FR (2) FR2955333B1 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2616318A1 (fr) * 1987-06-15 1988-12-16 Centre Nat Rech Scient Peau artificielle et son procede de preparation
GB9721585D0 (en) * 1997-10-10 1997-12-10 Geistlich Soehne Ag Chemical product
EP1254670A1 (fr) * 2001-05-03 2002-11-06 Stichting Katholieke Universiteit Matrices de collagène II pour la régéneration de cartilage

Also Published As

Publication number Publication date
FR2955334B1 (fr) 2013-09-06
FR2955334A1 (fr) 2011-07-22
FR2955333A1 (fr) 2011-07-22

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