FR2860976A1 - Use of oral synergistic composition for treating e.g. cerebral or cardiac dysfunction, contains omega-3 fatty acids, at least two vitamins and at least two trace elements - Google Patents

Abstract

Use of an oral synergistic composition (A) for improving the bioavailability and efficacy of omega-3 polyunsaturated fatty acids (I). (I) is associated with a complex (X) that activates cellular metabolism and contains at least two vitamins (A, B2, B9 and E) and at least two minerals or trace elements (magnesium, zinc, copper and selenium), to stimulate neuronal function. ACTIVITY : Cardiant; Neuroprotective; Cerebroprotective; Antidepressant; Tranquilizer; Nootropic; Anorectic. MECHANISM OF ACTION : Possibly Vitamin E and selenium inhibit oxidation of (I), while the other components of (X) are catalysts/co-enzymes involved in the metabolism of neuronal cells and in synthesis of neurotransmitters. A monolayer (10% confluent) of PC2 neuronal cells was cultured in a nutrient medium that was not replaced and the density (cells/mm2>) of the culture fell from 7618 (day 7) to zero (day 21). When the culture was treated with omega-3 fatty acids (I), the density after 21 days was 1152, but when cultures were treated with (I) and a mixture containing Vitamins A, B2, B9 and E, plus magnesium, zinc, copper and selenium, the density after 21 days was 4736 (concentrations of the various treatment compounds are not stated).

Description

2860976 1

  Our diet contains different categories of nutrients: carbohydrates, proteins, fats, vitamins, minerals and trace elements. These nutrients provide energy to maintain all cellular activities of the body. Cells need energy to function but they also need these nutrients to synthesize cellular components, maintain cell membrane activity, synthesize specific substances like hormones and neurotransmitters, to allow cell growth and proliferation .

  When our diet brings all the necessary elements in the right proportions, the cells function normally and we are in good health. Inappropriate or decreased cell function results in a disorder or disease.

  For example, when the nerve cells do not receive adequate amounts of essential nutrients, the activity of cell membranes, the synthesis of neurotransmitters are disrupted and this manifests itself in the form of minor disorders such as a decrease in memory, depression, or by psychological disorders in the most severe cases.

  Like all organs in our body, the human brain is made up of specialized cells called neurons. From a morphological point of view, the brain can be divided into two distinct parts: the deepest, smallest and most ancestral part is called the limbic region. The outermost, voluminous and highly structured part is called neocortex. The limbic region of the human brain that controls emotional activity is found in many mammals while the neocortex has developed during the different phases of mammalian evolution. The neocortex is the seat of thought, reasoning and decision-making that are particularly developed in humans. It is now clear that most emotional, psychiatric or psychological disorders such as depression are related to disturbances in the limbic region.

  About 70% of our brain's tissues are composed of fatty acids. These fatty acids are derived from the fats of our diet. From the chemical point of view, fat molecules, insoluble in water, result from the combination of an alcohol, glycerol, with fatty acids consisting of chains of 4 to 22 carbon atoms bound to atoms of hydrogen. When all the carbon atoms are joined by a single C-C bond, it means that all the other bonds are occupied by hydrogen atoms.

  Since these carbon atoms can not accept new hydrogen atoms, the corresponding fatty acids are said to be saturated. Saturated fatty acids are usually of animal origin (butter, milk, meat). When the carbon atoms of the fatty acids are linked by C = C double bonds that can accept new hydrogen atoms, these are called unsaturated fatty acids. If the fatty acid has only one double bond, it is said monounsaturated (example: oleic acid) and when it has more than one double bond, it is said to be polyunsaturated. Polyunsaturated fatty acids oxidize very easily in the body because they are highly unstable and require the presence of antioxidants to prevent their rapid degradation by oxidation in the body. The position of the first double bond (called omega) makes it possible to distinguish the omega-3 polyunsaturated fatty acids (example: alpha-linolenic acid) and the omega-6 polyunsaturated fatty acids (example: linoleic acid). These types of fatty acids are said to be essential because they can not be synthesized by the body and must be provided by the diet. Omega-6 are present in corn, sunflower and sesame oil which are widely used. Omega-3s are found in flaxseed oil, nuts, mustard oil, soy, wheat and in the flesh of cold-water fish and are little or not at all consumed in our modern societies.

  Fatty acids allow the formation of phospholipids in biological membranes. The membrane fluidity that is crucial for its functioning depends on its lipid composition. Omega3 fatty acids increase membrane fluidity because double bonds prevent membrane compaction.

  The cell membranes of the limbic region of the brain have a very high content of omega-3 polyunsaturated fatty acids. As these fatty acids are provided by the diet, any dietary deficiency has immediate consequences on the development of neuronal membranes, cellular enzymatic activities, synthetic cell activities and neurotransmission. Because the limbic region controls emotions, it has a great influence on heart function (emotions can speed up the heart rate). Thus, the omega-3 fatty acid deficiency that affects brain function can also affect heart function.

  The reason why the limbic region of the brain is rich in polyunsaturated fatty acids is unclear. It is generally accepted that the limbic brain developed at the very beginning of evolution, when the first living beings evolved in aquatic environment and had a marine diet rich in omega-3 polyunsaturated fatty acids. Unfortunately, most of the food consumed by men in developed countries is low in polyunsaturated fatty acids, particularly omega-3. Under ideal conditions, our food should contain omega-3 and omega-6 fatty acids in a ratio of 1: 1. This ratio is usually 1:10 in common foods.

  The frequency of depression, anxiety, stress, and psychiatric problems seems to have increased steadily since the beginning of the 20th century. Although various factors may have contributed to this evolution, numerous scientific and epidemiological studies show that these disorders are related to the Western world's dietary habits, in which omega-3 polyunsaturated fatty acids contained in seafood, oils and of fish (obtained from fish skin fat) or flaxseed oil have been largely replaced by oily oils rich in saturated fatty acids or omega-6 polyunsaturated fatty acids. Many recent clinical studies (Kalminj et al., Annals of Neurology, vol 42, 1997, Peet M. et al., Arch Gen. Psychiatry, vol 59, 2002, Stoll AL et al., Arch Gen. Psychiatry, vol 56, 1999; Tanskanene A et al., Gen. Psychiatr, vol 29, 2003. Colin A. et al., Encephale, vol 29, 2003, Maes et al., Psychyatry Res., Vol 85, 1999) clearly show the direct link between omega-3 deficiency in the diet and many mental disorders.

  Despite the existence of this direct link between the omega-3 deficiency and the increase in the frequency of psychic disorders and cardiovascular diseases, the use of omega-3 to restore normal cellular functions has not occurred. widespread. This lack of interest in omega-3 is due to three main causes: 1. It is necessary to consume large quantities of omega-3-rich foods such as oily fish (salmon ...) or oil. of flax, which is not practical, 2. Even if the best sources of omega-3 like fish oil capsules are concentrated in omega-3, it is necessary to take at least 4 to 6 capsules of large size per day, which is not easy, 3. The results obtained with varying qualities of oils rich in omega-3 are often not encouraging.

  It is for this reason that a large part of the population of Western developed countries suffers from minor psychic problems and renounces the regular use of omega-3s.

  It has now been clearly established that a minimum of vitamins, minerals and trace elements are essential to ensure optimal cellular metabolic functions. Many vitamins, minerals and trace elements act as co-enzymes or catalysts in many intracellular biochemical reactions. Since these elements are essential for optimal cellular metabolism, they are called vital elements. Since they are not synthesized or stored properly in the body, we need to take a minimum of Recommended Daily Allowance (RDA) of these elements each day. A normally functioning cell transforms various foodborne substances into energy, hormones, enzymes or neurotransmitters through the action of vital elements that act as catalysts or co-enzymes in the chains of intracellular biochemical reactions. The lack of only one of them vital disrupts all the reaction chains and decreases cellular metabolism. This decrease in cellular functions is responsible for the appearance of various health problems such as depression, when it is the nerve cells that are affected, the lack of vitality, when it comes to muscle cells, sexual problems when it is hormonal cells. . . etc. Numerous epidemiological studies show that mental problems and psychiatric illnesses have continuously increased since the beginning of the 20th century and that the wealth of vital elements of our diet is constantly decreasing. It has also been shown that 7 out of 10 people in developed countries do not receive the RDA (Minimum Daily Intake) minimum of one or more vital elements. The correlation between this vital deficiency in 7 out of 10 people and the frequency of minor or major mental disorders in about 70% of the population in developed countries has never been clearly established.

  The purpose of our research was to find the elements that stimulate the cellular metabolic activity of nerve cells to improve the bioavailability and activity of omega-3 (or omega-3) polyunsaturated fatty acids. We have developed cellular models in vitro in which cellular metabolic activities are artificially reduced by decreasing the amounts of vital elements in the culture media. Various vital elements were then added to the culture media, alone or in combination with each other, to study the effect on cellular metabolic functions. Surprisingly, we have observed that vitamins, minerals and trace elements do not stimulate cellular metabolism when they are brought in identical proportions (equivalent to 50% of RDA) in the culture medium but only some of them, in particular proportions, stimulate cellular metabolic functions. This specific combination contains at least two vitamins chosen from vitamins A, B2, B9 and E with at least two minerals or trace element selected from magnesium, zinc, copper and selenium. The best results were obtained when the 8 elements were brought together (see Table 1). The additional addition of other vital elements can slow down cellular metabolism.

  We observed that the contribution of omega-3 alone increased the in vitro nerve cell growth by + 7.8% compared to the control cultures. The combination of omega-3 with the vital elements that stimulate cellular metabolism increases the growth rate of nerve cells by + 38.7% (see Table 2).

  In vitro pharmacological studies on neuronal cells also show that omega-3s alone increase cell life by 15% to 20% and that the combination of omega-3s with vital elements stimulating cellular metabolism greatly increases the duration of life of neuronal cells (+ 62%).

  The in vivo pharmacological results also confirm a significant decrease in anxiety, an increase in memory and fitness parameters.

  These results clearly show that the combination of vitamins, minerals and trace elements, specific activators of cellular metabolism, with omega-3 polyunsaturated fatty acids, significantly increases the bioavailability of fatty acids, thus restoring the functions of the fatty acids. membranes and synthetic activity of nerve cells. Symptoms of disorders related to neuronal function decrease.

  Although the mode of action of this synergistic association is not clearly defined, it is reasonable to imagine that vitamin E and selenium act as specific antioxidants to protect polyunsaturated fatty acids and reduce their oxidation and degradation in the body. Similarly, vitamins A, B2, B9, magnesium and copper act as specific catalysts or coenzymes in neuronal cell metabolism, contributing to the regeneration of neuronal cell membranes and the correct synthesis of neurotransmitters by restoring normal cellular functions. The correct association of the vital elements mentioned above is very specific, because the excess of one of the elements can saturate certain sites of the cellular receptors by nonspecific bonds, which can lead to the under use of another element. . This slows down the functions of cellular metabolism. It is therefore essential to provide not only a sufficient amount of omega-3 but also to stop their oxidation and degradation in the body while stimulating the functions of cellular metabolism which will optimize the activity of omega-3. 3.

  The polyunsaturated fatty acids according to the invention can be obtained from any natural or synthetic source such as fish oil, linseed oil, microorganisms, plants or animals genetically modified or not, eggs, butter, condensed milk, especially fish oils from cold waters.

  Sources of polyunsaturated fatty acids can be concentrated in omega3 by centrifugal partition chromatography or by any other technique to decrease the proportion of omega-6.

  Under preferred conditions according to the invention, the ratio between omega-3 and omega-6 is from 1: 1 to 1: 7.

  Under preferred conditions of use according to the invention, the source of omega-3 is a fish oil concentrated in omega-3 administered orally in the form of soft gelatin-based capsules.

  The daily doses of omega-3 depend on their destination and may vary between 100 mg and 1.5 g of omega-3 per day, the quantities supplied being preferably between 500 and 1000 mg of omega-3 per day, in one or several takes.

  The specific vital elements according to the invention can be obtained from any natural or synthetic source of vitamins, minerals and trace elements, in liquid form or in powder form.

  The specific vital elements can be associated with omega-3 sources of polyunsaturated fatty acids either by mixing the different sources together, or by providing vitamins and / or minerals and / or trace elements separately with capsules, capsules , tablets, powders or syrups with an omega-3 enriched source.

  The specific complex of vital elements activating the cell metabolism can be brought at the same time as the omega-3 or can be brought at any other time of the day. It is also possible to administer the omega-3 and the complex of vital elements by alternating days.

  According to the invention, the synergistic association of omega-3 with the complex of vital elements activating cellular metabolism can be used as a preventive or curative treatment for disorders of cerebral functioning such as depression, anxiety, losses. Memory, Dementia, Alzheimer's Disease, Insomnia, Fatigue, Age-Related Aging and Brain Function Disorders, Obesity, and Vitality in Men and in women.

  Other vitamins (such as vitamin C, vitamin D), minerals (such as calcium) or trace elements (such as iodine, iron) can be integrated into the complex of specific vital elements stimulating functions. because they do not interfere with the activity of the complex.

  This combination can be presented in the form of a dietary product, a food supplement, a herbal product, a medicine or a cosmetic product.

  The usual dose of vitamins, minerals and trace elements, which varies according to the composition chosen, may for example be administered daily for a period of at least 14 days, preferably 30 days, at doses of between 15 and 200 percent of the intakes. Recommended Daily Allowance (RDA) for each of the items, specifically 50% of the RDAs. By RDA means the amount of micronutrients actually available for use by the body and not the gross weight of the form of intake used. It is advisable to use the combination of omega-3 with the specific complex of vitamins, minerals and trace elements every day and for all life except in case the diet provides 100% of the RDA. of each of these elements.

  Under preferential conditions according to the invention, the amounts of each of these elements used are, for example, 62.5% of the vitamin A RDAs, of 35.6% of the vitamin B2 RDAs, of 82.5%, % RDA vitamin B9, 67.5% RDA vitamin E, 50.3% RDA magnesium, 56.3% RDA zinc, 49.9% RDA copper, 40% RDA selenium.

  The following examples illustrate the present application. These examples of compositions for improving the bioavailability of omega-3 have been developed on the basis of the results obtained during the pharmacological studies.

  Some compositions were then subjected to clinical trials.

EXAMPLE 1

  Soft capsules based on an omega-3 enriched oil, vitamins, minerals and trace elements were prepared having the following weight formula: Fish oil concentrated to 60% omega-3 (ratio omega-3 / omega-6 = 2.5) 500 mg Vitamin A (beta carotene) 3.330 mg Vitamin B2 (riboflavin) 0.574 mg Vitamin B9 (folic acid) 0.166 mg Vitamin E (d-alpha-tocopherol) 10.058 mg Magnesium magnesium) 143.500 mg Zinc (zinc sulphate) 12.800 mg Copper (copper gluconate) 6.470 mg Selenium (fortified yeasts 2000 μg / g) 16.750 mg Excipient for soft capsules qs 1000 mg Dosage: 1 capsule each day with a glass of water, preferably in the morning.

EXAMPLE 2

  Soft capsules based on an omega-3 enriched oil, vitamins, minerals and trace elements were prepared having the following weight formula: Fish oil concentrated to 60% omega-3 (ratio omega-3 / omega-6 = 2.5) 500 mg Vitamin A (beta carotene) 3.330 mg Vitamin B2 (riboflavin) 0.574 mg Vitamin B9 (folic acid) 0.166 mg Vitamin C (ascorbic acid) 45.000 mg Vitamin E (d-alpha- tocopherol) 10.058 mg Magnesium (magnesium oxide) 143.500 mg Zinc (zinc sulfate) 12.800 mg Copper (copper gluconate) 6.470 mg Selenium (fortified yeasts 2000 μg / g) 16.750 mg Iodine (potassium iodide) 0.090 mg Iron (sulfate of iron) 19.810 mg Excipient for soft capsules qs 1000 mg Dosage: 1 capsule each day with a glass of water, 5 in the morning preferably.

EXAMPLE 3

  Soft capsules based on an omega3 enriched oil, vitamins, minerals and trace elements having the following weight formula were prepared: Omega-3 concentrated linseed oil 500,000 mg Vitamin A (beta carotene) ) 3.330 mg Vitamin B2 (riboflavin) 0.574 mg Vitamin B9 (folic acid) 0.166 mg Vitamin E (d-alpha-tocopherol) 10. 058 mg Magnesium (magnesium oxide) 143.500 mg Zinc (zinc sulfate) 12.800 mg Copper (gluconate) of copper) 6.470 mg Selenium (fortified yeasts 2000 pg / g) 16.750 mg Excipient for soft capsules qs 1000 mg Dosage: 1 capsule each day with a glass of water, preferably 25 in the morning.

EXAMPLE 4

  Soft capsules based on an omega-3 enriched oil used in combination with a syrup enriched with vitamins, minerals and trace elements and having the following weight formula were prepared: Soft capsules enriched in omega-3: fish concentrated to 60% in omega-3 (omega-3 / omega-6 ratio = 2.5) 500 mg Excipient for soft capsules qs 669 mg Syrup fortified with vitamins, minerals and trace elements: Quantity of element per daily dose Vitamin A (beta carotene) 3.3300 mg Vitamin B2 (riboflavin) 0.8000 mg Vitamin B9 (folic acid) 0.1000 mg Vitamin E (source at 50% vitamin E) 10.0000 mg Magnesium (magnesium oxide) 248.7000 mg Zinc (zinc oxide) 9.3350 mg Copper (copper oxide) 1.2520 mg Selenium (sodium selenate) 0.0822 mg Excipient qsp one dose (approx. 7g) Dosage: 1 soft capsule daily with a glass of water, preferably in the morning used with one dose per day of vitamin enriched syrup, mineral x and trace elements.

EXAMPLE 5

  Soft capsules based on an omega-3 enriched oil, used in combination with vitamin-enriched soft capsules and mineral-enriched soft capsules, were prepared according to the following weight formulas: omega-3 enriched soft capsules: Fish oil concentrated to 60% omega-3 (omega-3 / omega-6 ratio = 2.5) 500 mg Excipient for soft capsules qs 669 mg Soft vitamin capsules: Vitamin A (beta carotene) 3.3300 mg Vitamin B1 (hydrochloride of Thiamine) 0.7060 mg Vitamin B2 (riboflavin) 0.5740 mg Vitamin B3 (nicotinamide) 9.0330 mg Vitamin B5 (calcium D-pantothenate) 2.7100 mg Vitamin B6 (pyridoxine hydrochloride) 1.0060 mg Vitamin B9 (folic acid) 0.1660 mg Vitamin E ( d- alpha-tocopherol) 10.0580 mg Vitamin C (L-ascorbic acid) 45.0000 mg Excipient for soft capsules qs 399 mg Soft capsules of minerals: Magnesium (magnesium oxide) 143.500 mg Iron (iron sulfate) 19.810 mg Zinc (sulfate zinc) 12.800 mg Copper (copper gluconate) 1.252 mg Selenium (fortified yeasts 2000 μg / g) 16.750 mg Iodine (potassium iodide) 0.090 mg Excipient for soft capsules qs 631 mg Dosage: 2 soft capsules enriched with omega-3 , 1 soft capsule of vitamins and 1 soft capsule of minerals, with a glass of water, preferably in the morning.

  PHARMACOLOGICAL STUDIES: In vitro study of the role of omega-3 alone or in combination with vitamins, minerals and oliqo-elements on neuronal cell functions.

  The PC2 neuronal cell line has been used as a model of neuronal functioning. The cells were cultured according to the method described by Greene L. et al. (Adv.

  Cell Neurobiol., Vol 3, 373, 1982). The cells were seeded in 6-well tissue culture microplates containing RPMI 164 medium enriched with 10% heat-inactivated horse serum, fetal calf serum (5%), penicillin (50 IU / ml). streptomycin (50 μg / ml) and L-glutamine (2 μl / ml). The control cultures and cultures tested were incubated at 95% air and 5% CO2.

  The subcultures were prepared by coating the wells with 30 mm diameter cellulose mini-millets (of the milicell type) with poly-D-lysine and a matrigel by placing these inserts in Petri dishes of mm. diameter containing the culture medium. Cells were seeded at a density of 3000 cells per cm 2 and were finally differentiated by addition of S-NGF (50 μg / ml) in serum-free medium for 7 days. Nerve cells with axons and dendrites develop at 95% confluency in 7 days.

  The cells were trypsinized and multiplied under identical culture conditions for the different treatments For the various experiments, an omega-3 rich oil derived from fish skins (omega-3 / omega-6 ratio = 2.5: 1) was used. The oil was evenly distributed on one side of the cellulose inserts while the PC2 cells were grown on the other side. The maximum non-cytotoxic concentrations of omega-3 were determined prior to the different experiments.

  The vitamins and minerals were previously dissolved in stock solutions of culture medium and the appropriate dilutions were then introduced into the cultures of the neuronal cells. The cells were thus incubated for 3 to 10 days depending on the type of experiment. At the end of the experimental period, the mini-inserts of the culture plates were removed for the study of cellular parameters. The results were compared with the corresponding controls. All experiments were performed in triplicate (n = 6) with the same number of control cultures.

  a) effect of omega-3 on cell growth.

  In the starting cultures, an 80-90% monolayer culture is obtained in 7 days. The control cells were cultured in mini-inserts without omega-3 while the other mini-inserts were treated with omega-3. Vitamins and minerals were added to the culture media (up to 1/10 of the maximum non-cytotoxic concentrations). The number of cells was determined after trypsination at day 7. Only the most relevant results with vitamins (A, B2, B9, E), minerals (magnesium) and trace elements (copper, zinc, selenium) selected are presented. The following results were obtained.

Table 1:

  Experiment Mean Number of Percent Cells / cm2 at Day 7 (n = 18) Control Neural Cells 7800 100 Cells with 50% RDA in Vitamin A, B1, B2, B3, B5, B6, B8, B9, B12, C, E , magnesium, zinc, copper, 7914 101.46 selenium, iodine, iron, cobalt, manganese Cells with 50% RDA in vitamins A, B2, B9, 8642 110.80 E + magnesium, zinc, copper and selenium only Cells with omega-3 alone 8420 107.81 Cells with omega-3 + 8918 114.19 vitamins A, B2, B9, E Cells with omega-3 + magnesium, zinc, copper and 8778 112.39 selenium Cells with vitamins A, B2, B9, E + magnesium, zinc, copper and selenium alone 9438 121.00 Cells with omega-3 + vitamins A, B2, B9, E + magnesium, zinc, copper and selenium 10832 138.69 These results show that omega-3 increase the growth of neuronal cells (+ 7.81% compared with controls ). The addition of vitamins, minerals and trace elements to 50% of RDAs has no influence on cell growth while the selected vital elements further stimulate cell growth. The combination of omega-3 with specific vital elements strongly stimulates the growth of neuronal cells (+ 38.7%). b) Anti-aging effect The experimental procedure was identical to

  The previous experiment on cell growth except that after cell confluence (monolayer at 10% confluence), the culture medium was not renewed and cell mortality was determined at regular intervals until 24th day using neutral red staining, vital stain of living cells (colorless), and dead cells (red). The following results were obtained. 30

Table 2:

  Mean Number of Cells / cm2 Percent Survival Day 7 Day 14 Day 21 Day 14 Day 21 Control Neural Cells 4310 5108 5292 4936 5 652 6142 0.00 15.52 22.00 16.44 25.66 62.33 7618 7420 7318 7822 7740 7598 Cells with omega-3 10 cells only with omega-3 15 + vitamins A, B2, B9, E Cells with omega-3 + magnesium, zinc, copper and selenium Cells with 25 vitamins A, B2, B9, E + magnesium, zinc, copper and selenium only Cells with omega-3 + vitamins A, 35 B2, B9, E + magnesium, zinc, copper and selenium 56.57 68.84 72.31 63.10 73.02 80.84 These Results show that the maximum cell lifetime under standard conditions is less than 21 days but that this lifetime can be increased by 15 to 20% by providing omega-3 with specific vital elements. The intake of omega-3 with the selected vitamins, minerals and trace elements strongly reduces the death of neuronal cells and significantly increases the cell survival rate (+ 62%).

  In vivo pharmacology: The rats were divided into two groups. Group 1 consisting of adult male Wistar rats weighing about 200 g received a diet supplemented with an omega-3-rich oil (10 mg / day) with vitamins A, B2, B9 and E, magnesium, zinc, copper and selenium (40-50% of recommended daily intakes) for 30 days. Group 2 animals fed only omega-3-rich diets (10 mg / d) were the controls. The usual behavioral tests and the classical memory tests were conducted on days 28-30.

  In the two-compartment exploration test, group 1 showed less anxiety. In the forced swim test, the immobility time was shortened indicating a better physical condition in group 1. In the Maze test, the feeding time was shortened demonstrating an improvement in the memory in group 1. Finally in the scopolamine test, the duration of scopolamine-induced memory loss was decreased in the group receiving omega-3 and specific vital elements. These results prove that the combination of omega-3 with vitamins, minerals and micronutrients tested has an activity of improving the memory but also a restoring effect of the memory.

  Oral administration of omega-3 helps to improve the neurological functions of the brain. The combination of omega-3 with vitamins A, B2, B9, E, magnesium, selenium, zinc and copper has a synergistic effect on neuronal function and significantly increases the effect of ingredients taken separately.

  The vital element complex, omega-3 enriched fish oils and combinations of these two products were tested for one month in healthy volunteers and showed no side effects.

  These combinations can be used for the treatment of minor psychic disorders such as depression, anxiety, memory loss, to improve fitness and in anti-aging products or to extend the life span. The antioxidant properties of the complex of vitamins, minerals and trace elements stimulating cellular metabolism and the constitutive properties of omega-3 on the neuronal membranes are certainly at the origin of this synergistic activity.

Claims (5)

  1. Use of an orally synergistic composition for improving the bioavailability and efficacy of omega-3 polyunsaturated fatty acids, characterized in that it combines omega-3 with an activating complex of cell metabolism containing at least two vitamins chosen among vitamin A, vitamin B2, vitamin B9, vitamin E with at least two minerals or trace elements selected from magnesium, zinc, copper and selenium to stimulate neuronal functions.   2. Use of a synergistic oral composition for improving the efficacy of omega-3 polyunsaturated fatty acids, characterized in that it comprises an activator complex of cellular metabolic functions to improve the bioavailability of omega-3.   3. Use of an orally synergistic composition for improving the efficacy of omega-3 polyunsaturated fatty acids, characterized in that it comprises a source of natural origin rich in omega-3, such as fish oil from cold seas enriched in omega-3 with at least two vitamins selected from vitamin A, vitamin B2, vitamin B9, vitamin E with at least two minerals or trace elements selected from magnesium, zinc, copper and selenium by Oral route for the treatment of diseases caused by dysfunctions of cerebral or cardiac origin.   4. Oral use of polyunsaturated fatty acids with antioxidant vitamins, minerals and trace elements for the treatment of psychiatric disorders and cardiovascular disorders.   5. Use orally or locally of polyunsaturated fatty acids with at least two vitamins selected from vitamin A, vitamin B2, vitamin B9, vitamin E with at least two minerals or trace elements selected from magnesium , zinc, copper and selenium as a dietary, nutritional, pharmaceutical or cosmetic product.