FR2845244A1 - Production of a concentrate based on marine organisms useful in the pharmaceutical, food, cosmetics or textile domain comprises rearing larvae, harvesting the larvae at the trochophore stage, and packaging the larvae - Google Patents
Production of a concentrate based on marine organisms useful in the pharmaceutical, food, cosmetics or textile domain comprises rearing larvae, harvesting the larvae at the trochophore stage, and packaging the larvae Download PDFInfo
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- FR2845244A1 FR2845244A1 FR0301848A FR0301848A FR2845244A1 FR 2845244 A1 FR2845244 A1 FR 2845244A1 FR 0301848 A FR0301848 A FR 0301848A FR 0301848 A FR0301848 A FR 0301848A FR 2845244 A1 FR2845244 A1 FR 2845244A1
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- 239000012141 concentrate Substances 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- 230000000384 rearing effect Effects 0.000 title claims abstract 5
- 239000002537 cosmetic Substances 0.000 title claims description 5
- 239000004753 textile Substances 0.000 title claims description 3
- 238000003306 harvesting Methods 0.000 title abstract description 4
- 238000004806 packaging method and process Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 claims abstract description 18
- 210000000287 oocyte Anatomy 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 239000001963 growth medium Substances 0.000 claims description 14
- 238000009395 breeding Methods 0.000 claims description 13
- 230000001488 breeding effect Effects 0.000 claims description 13
- 230000035800 maturation Effects 0.000 claims description 10
- 239000013535 sea water Substances 0.000 claims description 10
- 239000002609 medium Substances 0.000 claims description 9
- 241000237852 Mollusca Species 0.000 claims description 6
- 241000243818 Annelida Species 0.000 claims description 5
- 241000258955 Echinodermata Species 0.000 claims description 5
- 241000237858 Gastropoda Species 0.000 claims description 4
- 230000001143 conditioned effect Effects 0.000 claims description 4
- 230000005484 gravity Effects 0.000 claims description 3
- 230000002503 metabolic effect Effects 0.000 claims description 3
- 238000004062 sedimentation Methods 0.000 claims description 3
- 238000002604 ultrasonography Methods 0.000 claims 1
- 235000008504 concentrate Nutrition 0.000 description 11
- 229920002101 Chitin Polymers 0.000 description 7
- SBJKKFFYIZUCET-JLAZNSOCSA-N Dehydro-L-ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(=O)C1=O SBJKKFFYIZUCET-JLAZNSOCSA-N 0.000 description 4
- SBJKKFFYIZUCET-UHFFFAOYSA-N Dehydroascorbic acid Natural products OCC(O)C1OC(=O)C(=O)C1=O SBJKKFFYIZUCET-UHFFFAOYSA-N 0.000 description 4
- 235000020960 dehydroascorbic acid Nutrition 0.000 description 4
- 239000011615 dehydroascorbic acid Substances 0.000 description 4
- 230000004720 fertilization Effects 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000013543 active substance Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 241000283153 Cetacea Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/50—Molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cosmetics (AREA)
Abstract
Description
l Procédé de fabrication d'un concentré à base d'organismes d'originel Process for the manufacture of a concentrate based on original organisms
marine tels que des mollusques, des échinodermes et des annélides destiné à être utilisé dans le domaine pharmaceutique, alimentaire, cosmétique ou textile La présente invention vise un procédé de fabrication d'un concentré à base d'organismes d'origine marine tels que des mollusques, des échinodermes et des annélides, destiné à être utilisé dans le domaine pharmaceutique, alimentaire, The present invention relates to a process for the production of a concentrate based on organisms of marine origin such as molluscs. The present invention relates to a process for the manufacture of a concentrate based on organisms of marine origin such as molluscs. , echinoderms and annelids, intended for use in the pharmaceutical, food,
cosmétique ou textile.cosmetic or textile.
Il est connu d'utiliser des organismes marins pour obtenir des substances biologiques actives telles que la chitine. It is known to use marine organisms to obtain active biological substances such as chitin.
Ainsi, le brevet EP-B-0 869 805 décrit un procédé de 30 préparation de substances actives essentiellement non minérales à partir de la nacre pour fabriquer ensuite des médicaments. Thus, patent EP-B-0 869 805 describes a process for the preparation of essentially non-mineral active substances from mother-of-pearl to then manufacture medicaments.
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On connaît également les travaux de Taylor et al, publiés dans le bulletin du British Museum Suppl. 1.125pp.+29 plates, 1969, concernant la nacre ou l'aragonite issue des coquilles We also know the work of Taylor et al, published in the bulletin of the British Museum Suppl. 1.125pp. + 29 plates, 1969, concerning mother-of-pearl or aragonite from the shells
d'organismes marins.of marine organisms.
Ces deux publications préconisent une extraction mécanique des substances aboutissant à une pulvérisation à partir de laquelle sont extraites diverses substances biologiquement actives par divers procédés chimiques. Ces procédés 10 nécessitent une pulvérisation de la nacre de la coquille de These two publications recommend a mechanical extraction of the substances leading to a spraying from which various biologically active substances are extracted by various chemical processes. These methods require spraying the mother-of-pearl from the shell.
l'organisme marin tel que le bivalve. the marine organism such as the bivalve.
Avant de passer au stade adulte, de tels organismes marins se présentent sous forme de larves. Ces larves constituent en 15 milieu naturel ce que l'on nomme le zoo plancton. Ce zoo Before moving to the adult stage, such marine organisms appear in the form of larvae. These larvae constitute in a natural environment what is called the zoo plankton. This zoo
plancton est un aliment dont la richesse justifie, en particulier, les grandes transhumances des grands cétacés. plankton is a food whose richness justifies, in particular, the great transhumance of large cetaceans.
Ces déplacements prouvent en eux-mêmes l'intérêt que les mammifères ont à consommer ce zoo plancton. 20 La présente invention résulte de cette observation et a pour but de proposer un nouveau procédé de fabrication d'un concentré à base d'organismes marins lorsque ceux-ci se These movements prove in themselves the interest that mammals have in consuming this zoo plankton. The present invention results from this observation and aims to propose a new method for manufacturing a concentrate based on marine organisms when these are
trouvent au stade de larve.found in the larva stage.
Ainsi, l'invention objet de la présente demande consiste en un procédé de fabrication d'un concentré à base d'organismes d'origine marine, notamment de mollusques tels que les bivalves et les gastéropodes, d'échinodermes et d'annélides, 30 caractérisé en ce qu'il consiste à élever des larves desdits organismes marins dans un milieu d'élevage, tel que de l'eau de mer, et à récolter lesdites larves d'organismes marins lorsqu'elles se trouvent au stade trochophore, lesdits Thus, the invention which is the subject of the present application consists of a process for the manufacture of a concentrate based on organisms of marine origin, in particular molluscs such as bivalves and gastropods, echinoderms and annelids, 30 characterized in that it consists in raising larvae of said marine organisms in a culture environment, such as sea water, and in collecting said larvae of marine organisms when they are in the trochophore stage, said
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trochophores étant ensuite séparés du milieu d'élevage puis conditionnés. Selon une forme de réalisation, on interrompt le processus 5 d'évolution métabolique des larves lorsqu'elles sont au stade trocophore par un abaissement brutal de la température du milieu d'élevage et on récolte lesdits trocophores après leur trochophores then being separated from the culture medium and then conditioned. According to one embodiment, the process of metabolic evolution of the larvae is interrupted when they are in the trocophore stage by a sudden lowering of the temperature of the culture medium and the said trocophores are harvested after their
sédimentation par gravité.gravity sedimentation.
Selon une autre forme de réalisation du procédé selon According to another embodiment of the method according to
l'invention, les larves arrivées au stade trocophore sont stimulées de manière à nager en surface du milieu d'élevage, un courant circulaire étant généré à la surface du milieu d'élevage pour créer un tourbillon (vortex) dans lequel les 15 trocophores sont concentrés pour collecte. the invention, the larvae which have reached the trocophore stage are stimulated so as to swim on the surface of the culture medium, a circular current being generated on the surface of the culture medium to create a vortex (vortex) in which the 15 trocophores are concentrates for collection.
Ainsi, de manière avantageuse, on obtient facilement et sans opération mécanique au contraire de l'état de la technique, un concentré riche en éléments protéiniques, gluicidiques et 20 en éléments minéraux sensiblement équivalent au zooplancton. Thus, advantageously, one obtains easily and without mechanical operation, contrary to the state of the art, a concentrate rich in protein, gluicidic and mineral elements substantially equivalent to zooplankton.
Une fois, les trochophores collectés, on réalise une Once, the trochophores collected, we realize a
filtration tangentielle puis une concentration. tangential filtration then a concentration.
L'analyse du concentré obtenu permet de constater qu'il contient des protéines et également un polysaccharide appelé chitine et qu'il présente une forte teneur en DHA (acide déshydroascorbique), éléments d'une valeur intéressante pour une utilisation dans les divers domaines envisagés. 30 La chitine est présente dans ce concentré car elle est secrétée par la larve au stade trochophore pour fabriquer la coquille de l'organisme marin. Le procédé de fabrication Analysis of the concentrate obtained shows that it contains proteins and also a polysaccharide called chitin and that it has a high content of DHA (dehydroascorbic acid), elements of interesting value for use in the various fields envisaged. . 30 Chitin is present in this concentrate because it is secreted by the larva at the trochophore stage to make the shell of the marine organism. The manufacturing process
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selon l'invention permet avantageusement d'obtenir cette chitine sans recourir au broyage des coquilles d'organismes marins comme cela était le cas précédemment. En outre, la according to the invention advantageously makes it possible to obtain this chitin without having to grind the shells of marine organisms as was the case previously. In addition, the
chitine est obtenue très pure.chitin is obtained very pure.
Cette chitine est un polymère naturel qui possède une structure très approchante des mucoploysaccharides (héparine, acide hyaluronique) dont la tolérance biologique est démontrée. Le DHA présente également de nombreuses applications, en particulier dans le domaine pharmaceutique et alimentaire puisqu'il est indispensable au bon fonctionnement du cerveau humain. L'invention vise également un concentré d'organismes d'origine marine, notamment de mollusques tels que les bivalves et les gastéropodes, d'échinodermes et d'annélides utile dans l'industrie pharmaceutique, cosmétique et 20 alimentaire, caractérisé en ce qu'il est constitué d'au moins This chitin is a natural polymer which has a structure very close to the mucoploysaccharides (heparin, hyaluronic acid) whose biological tolerance has been demonstrated. DHA also has many applications, in particular in the pharmaceutical and food sector since it is essential for the proper functioning of the human brain. The invention also relates to a concentrate of organisms of marine origin, in particular of molluscs such as bivalves and gastropods, of echinoderms and of annelids useful in the pharmaceutical, cosmetic and food industry, characterized in that it consists of at least
un isolat de larves desdits organismes, au stade trochophore. an isolate of larvae of said organisms, in the trochophore stage.
Le concentré obtenu selon le procédé de l'invention est en particulier riche en protéines, en DHA et en chitine et peut 25 se présenter sous forme d'un sirop, d'une pâte pour The concentrate obtained according to the process of the invention is particularly rich in proteins, DHA and chitin and can be in the form of a syrup, a paste for
l'industrie cosmétique, pharmaceutique ou alimentaire. cosmetic, pharmaceutical or food industry.
De préférence, l'élevage s'effectue de la manière suivante. Preferably, the breeding is carried out in the following manner.
On sélectionne les organismes marins selon leur sexe pour en 30 récolter séparément les ovocytes et le sperme. Les ovocytes sont ensuite dilués dans un volume d'eau de mer de manière que leur concentration soit comprise entre 3 et 30 par millilitre. On utilise de préférence des bacs de 8 à 15 Marine organisms are selected by sex to collect oocytes and sperm separately. The oocytes are then diluted in a volume of sea water so that their concentration is between 3 and 30 per milliliter. It is preferable to use trays from 8 to 15
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litres environ pour réaliser la fécondation qui se produit en mélangeant de 10 à 15 millilitres d'eau de mer contenant de 50 à 250 spermatozodes par millilitre. Généralement la taille des spermatozodes étant inférieure de 2 à 7 fois à 5 celle des ovocytes, dans un même volume d'eau, le nombre de spermatozodes sera 2 à 7 fois supérieur à celui des ovocytes. Les bacs sont mis à l'écart environ pendant une période de 10 temps de 1 à 3 heures dans de l' eau de mer à température approximately liters to carry out the fertilization which occurs by mixing 10 to 15 milliliters of sea water containing 50 to 250 sperm per milliliter. Generally the size of the sperm being 2 to 7 times less than that of oocytes, in the same volume of water, the number of sperm will be 2 to 7 times greater than that of oocytes. The tanks are placed aside for approximately a period of 10 times from 1 to 3 hours in seawater at temperature
ambiante, non inférieure à 10 Celsius, pour assurer une fécondation optimale. Après cette période, on sépare les ovocytes fécondés des spermatozodes en tamisant le volume du bac de fécondation sur un tamis dont les trous ne sont pas 15 supérieurs à 25 microns. ambient, not less than 10 Celsius, to ensure optimal fertilization. After this period, the fertilized oocytes are separated from the sperm by sieving the volume of the fertilization tank over a sieve whose holes are not more than 25 microns.
Un volume d'eau de mer qui constituera le milieu d'élevage pour les ovocytes fécondés, est porté à une température généralement comprise entre 10 et 30 Celsius, de préférence 20 entre 20 et 240Celsius. On disperse les ovocytes fécondés dans le milieu d'élevage et on assure un brassage du milieu d'élevage durant la phase de maturation des ovocytes fécondés. La durée d'évolution pour atteindre le stade trochophore varie entre 4 et 15 heures et elle est 25 conditionnée par la température du milieu d'élevage, diminuant avec son augmentation thermique. Une fois que la température du milieu d'élevage souhaitée est atteinte, le brassage cesse et la température est maintenue stable. Les larves nagent alors librement dans le milieu d'élevage. 30 Entre la 4ème et la 12ême heure, on contrôle l'évolution des larves. Lorsque le stade trochophore est constaté, le processus de récolte est engagé. Ainsi, les larves sont A volume of seawater which will constitute the culture medium for the fertilized oocytes, is brought to a temperature generally between 10 and 30 Celsius, preferably between 20 and 240 Celsius. The fertilized oocytes are dispersed in the breeding medium and the breeding medium is stirred during the maturation phase of the fertilized oocytes. The evolution time to reach the trochophore stage varies between 4 and 15 hours and it is conditioned by the temperature of the culture medium, decreasing with its thermal increase. Once the desired temperature of the culture medium is reached, brewing stops and the temperature is kept stable. The larvae then swim freely in the breeding environment. 30 Between the 4th and 12th hour, we check the evolution of the larvae. When the trochophore stage is noted, the harvesting process is started. So the larvae are
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amenées en surface du milieu d'élevage à l'aide de moyens de stimulation tels qu'un émetteur ultra-son, des micro-bulles brought to the surface of the breeding environment using stimulation means such as an ultrasonic transmitter, micro-bubbles
ou un courant d'eau placé au fond du bac de maturation. or a stream of water placed at the bottom of the maturation tank.
Des jets d'eau orientés mis en place sur le bord du bac de maturation génèrent un courant circulaire créant un tourbillon (vortex) dans lequel les larves sont concentrées Oriented water jets placed on the edge of the maturation tank generate a circular current creating a vortex (vortex) in which the larvae are concentrated
pour être collectées dans un décanteur. to be collected in a decanter.
Selon une variante, un abaissement brutal de la température à environ 150Celsius permet de bloquer l'évolution métabolique According to a variant, a sudden lowering of the temperature to around 150Celsius makes it possible to block the metabolic evolution
des larves.larvae.
On peut alors laisser les larves se déposer par gravité au 15 fond du bac de maturation o elles sont récoltées dans un décanteur. La concentration peut alors être évaluée par The larvae can then be left to settle by gravity at the bottom of the maturation tank where they are harvested in a decanter. The concentration can then be assessed by
observation de l'opacité et comptage au microscope. observation of opacity and counting under the microscope.
Tout autre procédé de récolte approprié peut être utilisé 20 dans le cadre de l'invention. Any other suitable harvesting method can be used in the context of the invention.
Une fois récoltées, les larves sont conditionnées soit par congélation soit par lyophilisation ou encore elles sont directement diluées dans des préparations avec adjonction 25 notamment de gel et conservateurs. Once harvested, the larvae are conditioned either by freezing or by lyophilization or they are directly diluted in preparations with the addition, in particular, of gel and preservatives.
La présente invention a également pour objet un dispositif pour la mise en oeuvre du procédé selon l'invention caractérisé en ce qu'il comporte un bac de maturation propre 30 à recevoir le milieu d'élevage et les ovocytes fécondés, ledit bac étant pourvu de moyens de chauffage, de moyens de stérilisation et de moyens de brassage dudit milieu d'élevage. The present invention also relates to a device for implementing the method according to the invention, characterized in that it comprises a maturation tank suitable for receiving the culture medium and the fertilized oocytes, said tank being provided with heating means, sterilization means and means for stirring said culture medium.
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De préférence, le dispositif comporte également des moyens de Preferably, the device also includes means for
collecte des larves vers un bac de décantation. collection of larvae to a settling tank.
On décrira maintenant l'invention plus en détail en référence au dessin dans lequel la figure unique représente une vue schématique en transparence d'un dispositif de mise en oeuvre The invention will now be described in more detail with reference to the drawing in which the single figure represents a schematic view in transparency of an implementation device.
du procédé selon l'invention.of the process according to the invention.
Le dispositif de mise en oeuvre du procédé selon l'invention The device for implementing the method according to the invention
comporte un bac de maturation 1 qui contient, en tant que milieu d'élevage, de l'eau de mer. Ce bac est pourvu de moyens de chauffage du milieu d'élevage, tels que quatre lampes infra-rouge, des résistances électriques en 15 porcelaine. comprises a maturation tank 1 which contains, as the breeding medium, sea water. This tank is provided with means for heating the breeding medium, such as four infrared lamps, electrical resistances in 15 porcelain.
Ainsi, on met en oeuvre un volume d'eau de mer dans un bac de maturation d'un volume de 1000 litres. Le milieu d'élevage est chauffé à l'aide des quatre lampes infra-rouges 2 de 20 manière à amener le milieu d'élevage à une température de Thus, a volume of seawater is used in a maturation tank with a volume of 1000 liters. The culture medium is heated using the four infrared lamps 2 so as to bring the culture medium to a temperature of
240Celsius qui optimise le développement des larves. 240Celsius which optimizes the development of larvae.
Le bac de maturation 1 est également pourvu de moyens de stérilisation 3 du milieu d'élevage, tels que des rampes UV. 25 Une pompe 4 permet d'alimenter le bac 1 et assure un brassage du milieu d'élevage pendant la phase de chauffage de celuici. Un thermostat permet de maintenir la température du The maturation tank 1 is also provided with means 3 for sterilizing the culture medium, such as UV ramps. 25 A pump 4 is used to supply the tank 1 and ensures mixing of the breeding medium during the heating phase of this. A thermostat keeps the temperature of the
milieu d'élevage.breeding environment.
Après sélection des sexes et fécondation dans un volume d'eau de mer, de préférence de 10 litres, à la température de 150Celsius, on filtre les ovocytes fécondés au travers d'un After selection of the sexes and fertilization in a volume of sea water, preferably 10 liters, at a temperature of 150 Celsius, the fertilized oocytes are filtered through a
8 28452448 2845244
tamis calibré de préférence à 25ju pour éliminer les sieve calibrated preferably at 25ju to eliminate
spermatozodes en excès.excess sperm.
Les ovocytes fécondés sont alors dispersés dans le volume 5 d'eau de mer contenu dans le bac de maturation 1. Les larves nagent librement dans le milieu d'élevage et la division cellulaire s'effectue ainsi jusqu'au stade de trochophore qui survient généralement au bout de 6 heures pour une The fertilized oocytes are then dispersed in the volume 5 of sea water contained in the maturation tank 1. The larvae swim freely in the breeding environment and cell division is thus carried out up to the trochophore stage which generally occurs after 6 hours for a
température donnée de 240Celsius.given temperature of 240Celsius.
Une rampe 5 pourvue de jets orientés permet de générer un mouvement circulaire du milieu d'élevage créant un siphon vers un collecteur 6 destiné à acheminer les larves vers un A ramp 5 provided with oriented jets makes it possible to generate a circular movement of the breeding medium creating a siphon towards a collector 6 intended to convey the larvae to a
bac de décantation 7.settling tank 7.
En variante, un refroidissement brutal de la température du milieu d'élevage à l'aide de moyens de refroidissement permet de bloquer l'évolution des trocophores. Ainsi un refroidissement jusqu'à 100C provoque l'arrêt du métabolisme 20 des larves qui cessent donc de nager et peuvent être As a variant, abrupt cooling of the temperature of the culture medium using cooling means makes it possible to block the evolution of the trocophores. Thus cooling to 100C stops the metabolism of the larvae, which therefore cease to swim and can be
récoltées par sédimentation dans le bac de décantation 7, le collecteur 6 guidant les trocophores vers ledit bac 7. harvested by sedimentation in the settling tank 7, the collector 6 guiding the trocophores towards said tank 7.
9 28452449 2845244
Claims (7)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0301848A FR2845244A1 (en) | 2002-10-03 | 2003-02-14 | Production of a concentrate based on marine organisms useful in the pharmaceutical, food, cosmetics or textile domain comprises rearing larvae, harvesting the larvae at the trochophore stage, and packaging the larvae |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0212291A FR2845243A1 (en) | 2002-10-03 | 2002-10-03 | Rearing marine bivalve, gastropod and echinoderm trochophores useful in pharmaceutical, food or cosmetic products comprises fertilizing and maturing oocytes under defined conditions |
| FR0301848A FR2845244A1 (en) | 2002-10-03 | 2003-02-14 | Production of a concentrate based on marine organisms useful in the pharmaceutical, food, cosmetics or textile domain comprises rearing larvae, harvesting the larvae at the trochophore stage, and packaging the larvae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| FR2845244A1 true FR2845244A1 (en) | 2004-04-09 |
Family
ID=32031844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR0301848A Pending FR2845244A1 (en) | 2002-10-03 | 2003-02-14 | Production of a concentrate based on marine organisms useful in the pharmaceutical, food, cosmetics or textile domain comprises rearing larvae, harvesting the larvae at the trochophore stage, and packaging the larvae |
Country Status (1)
| Country | Link |
|---|---|
| FR (1) | FR2845244A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2489510A (en) * | 2011-03-31 | 2012-10-03 | Peter Mcnulty | Carbon dioxide capture method and installation for cultivating shell-bearing gastropods |
| CN112602662A (en) * | 2020-12-30 | 2021-04-06 | 扬州大学 | Procambarus clarkii hatching device |
| US20210212300A1 (en) * | 2018-06-05 | 2021-07-15 | Bühler Insect Technology Solutions Ag | Large-Scale, High Density Storage of Larvae |
-
2003
- 2003-02-14 FR FR0301848A patent/FR2845244A1/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2489510A (en) * | 2011-03-31 | 2012-10-03 | Peter Mcnulty | Carbon dioxide capture method and installation for cultivating shell-bearing gastropods |
| US20210212300A1 (en) * | 2018-06-05 | 2021-07-15 | Bühler Insect Technology Solutions Ag | Large-Scale, High Density Storage of Larvae |
| CN112602662A (en) * | 2020-12-30 | 2021-04-06 | 扬州大学 | Procambarus clarkii hatching device |
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