FR2836827A1 - DIETETIC AND COSMETIC COMPOSITION BASED ON CROSSLINKED NATURAL POLYMERS IMMOBILIZING ACTIVE INGREDIENTS - Google Patents

Abstract

Composition for dietetic and cosmetic use based on at least one natural food polymer containing immobilized active principles capable of being obtained by crosslinking said polymer and by mixing it with depressant or osmosis products, process for obtaining such a composition.

Description

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DIETETIC AND COSMETIC COMPOSITION BASED ON CROSSLINKED NATURAL POLYMERS IMMOBILIZING ACTIVE INGREDIENTS.

Technical area
The invention relates to a dietary and cosmetic composition based on cross-linked natural food polymers in order to better release the active principles which they contain, its method of production and the main applications of the composition.

State of the art
One of the difficulties encountered by the formulator in the use of certain active ingredients lies in their good delivery so that the latter fulfill their beneficial roles.

These bio-availability problems are solved in many different ways depending on the nature of the asset. The technician or skilled in the art generally speaks of vectorization by means of capsules, liposomes, bio-vectors, complexing agents such as certain polysaccharides, structures which trap by confinement such as cyclo-dextrins or plant prolamins.

 Part of these techniques uses natural plant or animal polymers. There are those of structure like cellulose, those of function like starch. Quite recently, plant polymers such as prolamines have been the subject of development. But they are heavy and delicate to use. This is the case of US Patent 5,271,961.

 In the patent PCT / FR 96/00055, gels or polymeric films are produced from gliadin or wheat prolamine by adding plasticizers to them such as carbohydrates. The active substance is incorporated into the gel thus formed, which has to be dried. It is at this stage that this process encounters difficulties: the drying is done by heating and lasts too long for the stability of the sensitive substances, in particular the presence of water in the gel often degrades the active components and creates a risk of bacterial contamination, especially as the applications covered by this patent are pharmaceutical.

 The Applicant has successfully tried to overcome these difficulties in Application No. 0101624, by obtaining good results, without however mitigating the ulcerative allergic effects for the oral route, of the gluten contained in these formulations.

Knowing further that for topical cosmetics these formulas based in particular on vegetable prolamins cannot be used.

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 The present invention relates to a dietary and cosmetic composition based on natural food polymers containing active principles, its method of obtaining overcomes the drawbacks of current techniques, and thus making their uses possible, by facilitating their use and increasing their performance. in terms of bio-availability and stability of the active ingredients.

Brief description of the invention
The composition for dietetic and cosmetic use based on crosslinked natural polymers such as said polymers contains active principles which are introduced into the polymer gel before its crosslinking. The object of the invention is to obtain a composition in which the active ingredients are preserved or immobilized very quickly.

 Among the polymers capable of meeting the needs of the invention, they can be chosen from two main and nonlimiting families: - Animal or vegetable proteins such as gelatins and marine, bovine, porcine collagens, high molecular weight vegetable proteins, for example soy, lupine.

 The crosslinking agent will be for this family of proteins preferably chosen from tanning substances such as tannins or polyphenols from fruits such as procyanolic oligomers, flavonoids. Less commonly, chemical crosslinks may be used, such as PPC Phenol or enzymatic belonging to the polymerase family - Polyoses or polysaccharides of the chondroitin sulfate type from bovine, porcine, marine origin, gums such as guar gum, agar agar, hemi cellulose, chitin, mannan, poly galacturonate, xanthan, membrane polyosis.

 The crosslinking agent of this family will be a saline solution of the NaCl type or a solution of enzymes of the polymerase type such as for example the glucosyl transferase or enzyme mano transferase corresponding to the substrate.

 Among the active ingredients likely to be incorporated into these polymers, these are all those which encounter bad or insufficient bioavailability, such as certain vitamins, enzymes, trace elements poorly assimilated by the body such as phosphorus, calcium, magnesium. , like some irritating or ulcerative active ingredients like aspirin.

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 The Applicant is particularly concerned for its invention of enzymes conducive to immune defenses such as superoxydismutase, catalase, peroxidase alone or in combination, cytochrome.

The process for obtaining said compositions consists of: i) making an aqueous gel with the polymer ii) introducing the active ingredient with stirring in order to dissolve it or to suspend it in the gel iii) dispersing the gel into fine particles in a bath containing the crosslinking agent, as the case may be by the use of a milling agitator, by pressurized jets. iv) to separate by filtration or centrifugation the crosslinked particles or wires obtained in iii), v) to mix them with osmosis products such as dextrose vi) to dry in a ventilated oven regulated in temperature according to the thermosensitivity of the active
A variant can be used in certain cases:
Proceed i) and ii) as above and then: iii) mix the gel in the reverse osmosis unit. iv) Pour the cross-linking substance over the intimate mixture v) Dry as above.

 The product finally obtained in the two variants is a powder.

 The advantage of the reverse osmosis is to block the activity of water in the polymer gel by adding and mixing depressant or reverse osmosis products such as sugars.

The blocking of the water activity (Aw) is thus obtained by adding depressant or osmosis products to the polymer gel in quantities of between 1.5 and 15 times the amount of water by weight contained in the polymer gel. reticle. Experience shows that for an Aw equal to or less than 0.7 there is no appearance or development of molds, yeasts, bacteria.

 For certain uses such as in dietetics, the powder obtained can be very easily dispersed in water by disintegrating quickly while retaining the active particles enclosing the active agents in suspension. Likewise, such a suspension makes it possible to introduce into cosmetic compositions the particles of incorporated active agents.

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Ways to realize the invention
The invention, although simple in its description, will be well understood with the aid of the examples which illustrate its multiple applications and its advantages:
Example 1 Nutraceutical formulation for a 500 mg 10 mg tablet of dry fruit extract (cucurbits for example) containing SOD, dosed at activity at 20 units / mg, catalase dosed at 40 units / mg and peroxidase, mixed with 30 mg of marine gelatin gelled beforehand by adding water and heating. The gel is then sprayed by pressure through nozzles in a bath of purified grape tannins diluted in water at a rate of 10 g / liter of water. We observe the crosslinking which occurs instantly in the bath. The reticulate is collected on a stainless steel sieve canvas.

Finally 460 mg of anhydrous dextrose are added by mixing, ie 15 times the weight of water contained in the reticulate. A fine powder is obtained by drying at room temperature, which is compressed to 500 mg / unit.

Example 2
In place of the dextrose of Example 1, the depressant is made up of a percentage mixture of: 23% sucrose, 16% dextrose, 5% sorbitol, 5% sodium chloride, 1% citric acid, 50% ethyl alcohol.

In this case, the amount of depressants represents one tenth of the amount of water in the gel prepared as in Example 1.

 Example 3 In Vivo Tests with the Powder Obtained in Example 1: By Introducing It Into Dog Food for Exercise Dogs, 150 mg / Day and Measuring the Effective Increase in Endogenous SOD Activity on Erythrocyte Isolates from the Blood after 3 days. Better recovery of the animals is observed.

 Example 4 Food or Nutritional Supplementation With a powder such as that obtained in Example 1, a yogurt is added at the rate of 80 mg of powder.

 Example 5 Cosmetic emulsion: During the preparation of an anti-aging cream, 2% of powder is added as obtained in Example 1.

Claims (10)

claims 1 / Composition for dietetic and cosmetic use based on at least one plant polymer containing immobilized active principles capable of being obtained by crosslinking said polymer.  2 / Composition for dietetic and cosmetic use based on at least one plant polymer containing immobilized active principles obtained by crosslinking said polymer and then mixing it with depressant or osmosis products.  3 / dietetic and cosmetic composition according to claims 1 to 2 wherein the polymers are chosen from proteins, polyoses or polysaccharides.  4 / Dietetic and cosmetic composition according to claims 1 to 3 wherein the crosslinking products are chosen from natural tanning substances, chemical substances, and enzymes.  5 / dietetic and cosmetic composition according to claims 1 to 4 wherein the immobilized active is selected from the groups of trace elements, enzymes, enzymes, or vitamins.  6 / Dietetic and cosmetic composition according to claims 1 to 5 intended to immobilize antioxidants of enzymatic origins such as catalase, superoxydismutase or peroxidase, alone, together or in combination.  7 / A method of obtaining such a dietetic and cosmetic composition according to claims 1 to 6 consisting of: i) making with the polymer an aqueous gel ii) introducing the active with stirring in order to dissolve or put it suspended in the gel iii) dispersing the gel into fine particles in a bath containing the crosslinking agent, as appropriate by the use of a milling agitator, by pressurized jets. <Desc / Clms Page number 6>  iv) to separate by filtration or centrifugation the crosslinked particles or wires obtained in iii), v) to mix them with osmosis products such as dextrose vi) to dry in a ventilated oven regulated in temperature according to the thermosensitivity of the active ingredient .  8 / A method of obtaining such a dietetic and cosmetic composition according to claims 1 to 7, comprising: We proceed i) and ii) as above for claim 7 and then iii) mixing it in the reverse osmosis unit. iv) pour the crosslinking substance onto the mixture; v) dry as in claim 7.  9 / Use of the dietetic and cosmetic composition according to claim 6 in formulation to combat the effects of oxidative stress 10 / Use of the composition according to claim 6 for preparing a human or veterinary medicament for pathologies linked to oxidative stress.