FR2829484A1 - Agent for removing and preventing stains, useful for waste disposal systems, e.g. flush toilets, comprises at least one specified microorganism - Google Patents

Abstract

Antistaining agent (A) for waste disposal systems comprises at least one of the microorganisms (B) Lactobacillus paraceasei ssp. paracasei; Enterococcus malodoratus and Candida lipolytica. An Independent claim is also included for a method for preventing or removing stains in sanitary systems using (A).

Description

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 The present invention relates to an anti-stain agent for an evacuation system and a method for preventing / cleaning a stain of an evacuation system using this agent.

 A typical stain in drainage systems, particularly in toilet bowls, includes water stains, yellowish spots, blackish or brownish spots, and mineral deposits (poorly soluble calcium compounds produced by a reaction). between calcium ions and urine and phosphoric acid or carbon dioxide gas from the air). In a toilet bowl cleaning operation, a relatively small spot can be removed by applying a neutral toilet bowl cleaner to a stained portion, and rubbing the portion with a toilet brush or the like. To clean a stain attached to a toilet bowl, an alkaline cleaner containing a chlorine bleach is applied to a stained portion and rubbed with a toilet brush or the like. The whitening component can decompose the yellow or brownish stains and eliminate them by the cleaning operation. In addition, the mineral deposits attached to the toilet bowl may be removed by a method of dissolving deposits using an acidic detergent or a method of softening or dissolving deposits using microorganisms.

japonais mise à la disposition du public N 10-277587), ainsi qu'un appareil et une méthode d'élimination des taches et/ou des odeurs désagréables des cuvettes de toilettes (brevet japonais NO 3150299 ; publication de brevet japonais mise à la disposition du public NO 11-21973). Various techniques have been heretofore known for cleaning evacuation systems using microorganisms, such as fast-acting mineral deposition solvents (Japanese Patent Publication Laid-Open No. 2,237,688), a mineral deposit processing buffer (Japanese Patent Laid-open Publication No. 7-279215), a method of preventing a toilet bowl from an activity, to prevent or suppress the adhesion of mineral deposits (publication Japanese Patent Application Laid-Open No. 8-260552), a cleaning method and a flush toilet cleaning agent using micro-organisms (patent publication

Japanese Patent Publication No. 10-277587), and apparatus and method for removing stains and / or unpleasant odors from toilet bowls (Japanese Patent Publication No. 3150299; Japanese Patent Publication made available) Public Notice 11-21973).

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 An object of the present invention is to provide an anti-stain agent capable of preventing / cleaning a stain and removing an unpleasant odor from an evacuation system by using a microorganism that has not been used to prevent / eliminate stains from drainage systems. Another object of the present invention is to provide a method for preventing / cleaning a stain of an exhaust system using the above-mentioned stain control agent.

 The foregoing objects can be obtained by any one of the features (1) to (16) of the present invention.

 (1) A stain removal agent containing at least one strain of microorganisms selected from the group consisting of Lactobacillus paracasei subsp. paracasei and Enterococcus malodoratus; and Candida lipolytica.

 (2) An anti-spot agent as defined in (1), wherein the strains are held on a suitable support for immersing the strains in water.

 (3) An anti-stain agent as defined in (1) or (2), wherein the exhaust system comprises a toilet bowl and an exhaust pipe of a flush toilet system.

 (4) A stain removal agent containing three strains of microorganisms composed of Lactobacillus paracasei subsp. paracasei, Enterococcus malodoratus and Candida lipolytica.

(5) An anti-spot agent as defined under (4), wherein each of the three microorganism strains has the following ratio with respect to the total amount of the three strains of microorganisms:

<Tb>
<tb> Lactobacillus <SEP> paracasei <SEP> subsp. <SEP> paracasei <SEP>: <SEP> 20-60 <SEP>%
<tb> Enterococcus <SEP> Malodoratus <SEP>: <SEP> 20-60 <SEP>%
<tb> Candida <SEP> lipolytica <SEP>: <SEP> 10-30 <SEP>%.
<Tb>

 (6) An anti-spot agent as defined under (4) or (5), wherein the microorganisms are held on a suitable support for immersion of the strains in water.

 (7) An anti-stain agent as defined in (4) to (6), wherein the evacuation system comprises a toilet bowl and an evacuation pipe of a flush toilet system.

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 (8) A method of preventing or cleaning a stain of an evacuation system, wherein an anti-stain agent containing Candida lipolytica, and at least one strain of microorganisms selected from the group consisting of Lactobacillus paracasei subsp. paracasei and Enterococcus malodoratus, is brought into contact with water from the drainage system at one or more locations in the drainage system to prevent a stain from adhering to the drainage system or to decompose and remove the stain that adhered to the vent system.

 (9) A method as defined under (8), wherein the microorganisms are maintained on a suitable support for immersing the microorganisms in the water.

 (10) A method as defined in (8) or (9), wherein the exhaust system comprises a toilet bowl and an exhaust pipe of a flush toilet system.

 (11) A method as defined in (10), wherein the microorganisms are added to water in a water supply channel of a flush toilet system for subsequent contacting. with the water in the toilet bowl and the drain pipe.

 (12) A method of preventing or cleaning a stain of an evacuation system, wherein an anti-stain agent containing three strains of microorganisms composed of Candida lipolytica, Lactobacillus paracasei subsp. paracasei and Enterococcus malodoratus, is brought into contact with water in the drainage system at one or more locations in the drainage system to prevent adhesion of a stain to the system evacuate or decompose and remove a stain that adhered to the vent system.

 (13) A method as defined under (12), wherein the microorganisms are maintained on a suitable support for immersing the microorganisms in the water.

 (14) A method as defined in (12) or (13), wherein the exhaust system comprises a toilet bowl and an exhaust pipe of a flush toilet system.

 (15) A method as defined in (14), wherein the microorganisms are added to water in a supply channel

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 water flush toilet system to then be brought into contact with the water in the toilet bowl and the drain pipe.

(16) A method as defined under one of items (11) to (15), wherein each of the three microorganism strains has the following ratio with respect to the total amount of the three strains of microorganisms:

<Tb>
<tb> Lactobacillus <SEP> paracasei <SEP> subsp. <SEP> paracasei <SEP>: <SEP> 20-60 <SEP>%
<tb> Enterococcus <SEP> Malodoratus <SEP>: <SEP> 20-60 <SEP>%
<tb> Candida <SEP> lipolytica <SEP>: <SEP> 10-30 <SEP>%.
<Tb>

 Hereinafter, a specific embodiment of the present invention will be described.

 An anti-stain agent according to the present invention contains at least one strain of microorganisms selected from the group consisting of Lactobacillus paracasei subsp. paracasei and Enterococcus malodoratus, and Candida lipolytica. Preferably, the anti-stain agent contains three strains of microorganisms composed of Lactobacillus paracasei subsp. paracasei, Enterococcus malodoratus, and Candida lipolytica.

 Lactobacillus is a Gram-positive bacillus that causes sugar or saccharoid fermentation to produce essentially lactic acid. Cases are known in which Lactobacillus paracasei subsp. paracasei could be isolated from dairy products, wastewater, silage and clinical materials.

 It is preferred to use Lactobacillus paracasei subsp. paracasei which has been filed by the applicant internationally as DDD-a (FERM Acceptance Number BP-6463) with IPOD (International Patent Organism Depositary), AIST (Natioanl Institute of Advanced Industrial Science). and Technology), Japan, under the Bucharest Treaty.

The mycological properties of DDD-a of Lactobacillus paracasei subsp. paracasei will be explained later.

<Tb>
<Tb>

ELEMENT <SEP> TEST <SEP> RESULT <SEP> OF <SEP> TEST
<tb> Morphology <SEP> bacillus
<tb> Staining <SEP> of <SEP> Gram <SEP> +
<tb> Spore
<tb> Mobility
<Tb>

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<Tb>
<tb> Behavior <SEP> to <SEP> oxygen <SEP> anaerobiont <SEP> optional
<tb> Catalase
<tb><SEP> lactic acid <SEP> generated <SEP> L <SEP> (+)
<tb> Generation <SEP> of <SEP> gas <SEP> since <SEP><SEP> glucose
<tb> Generation <SEP> of <SEP> gas <SEP> since <SEP><SEP> gluconate <SEP> +
<tb> Growth <SEP> to <SEP> 15 <SEP> C <SEP> +
<tb> Growth <SEP> to <SEP> 45 <SEP> C
<tb> Fermentation
<tb> Amygdalin <SEP> +
<tb> Arabinose
<tb> Aesculine <SEP> +
<tb> Fructose <SEP> +
<tb> Galactose <SEP> +
<tb> Glucose <SEP> +
<tb> Gluconate <SEP> +
<tb> Lactose <SEP> +
<tb> Maltose <SEP> +
<tb> Mannitol <SEP> +
<tb> Mannose <SEP> +
<tb> Melezitosis <SEP> +
<tb> Melibiose
<tb> Raffinose
<tb> Rhamnose
<tb> Ribose <SEP> +
<tb> Salicine <SEP> +
<tb> Sorbitol <SEP> +
<tb> Sucrose <SEP> +
<tb> Trehalose <SEP> +
<tb> Xylose
<Tb>
Content by GC (mol%) of DNA in the mycobion (l 46 * 1 According to an HPLC method
Enterococcus is known as enterococcus, and a case is known in which Enterococcus malodoratus could be isolated from cheese.

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 It is preferred to use Enterococcus malodoratus which has been deposited by the applicant internationally as DDD-b (FERM Acceptance Number BP-6464) with IPOD (International Patent Organism Depositary), AIST (Natioanl Institute). Advanced Industrial Science and Technology), Japan, under the Bucharest Treaty.

The mycological properties of DDD-b of Enterococcus malodoratus will be explained later.

<Tb>
<Tb>

ELEMENT <SEP> TEST <SEP> RESULT <SEP> OF <SEP> TEST
<tb> Morphology <SEP> Streptococcus
<tb> Staining <SEP> of <SEP> Gram <SEP> +
<tb> Spore
<tb> Mobility
<tb> Behavior <SEP> to <SEP> oxygen <SEP> anaerobiont <SEP> optional
<tb> Catalase
<tb> Generation <SEP> of <SEP> gas <SEP> since <SEP><SEP> glucose
<tb><SEP> lactic acid <SEP> generated <SEP> L <SEP> (+)
<tb> Growth <SEP> to <SEP> 15 <SEP> C <SEP> +
<tb> Growth <SEP> to <SEP> 45 <SEP> C
<tb> Growth <SEP> in <SEP> presence <SEP> of <SEP> 6.5 <SEP>% <SEP> of <SEP> NaCl <SEP> +
<tb> Growth <SEP> to <SEP> pH <SEP> 9.6 <SEP> +
<tb> Growth <SEP> in <SEP> presence <SEP> of <SEP> 40 <SEP>% <SEP> of <SEP> bile <SEP> +
<tb> Haemolysis <SEP> a-hemolysis
<tb> Arginine <SEP> Dihydrolase
<tb> Acid <SEP> hippuric
<tb> Hydrolysis <SEP> of esculin
<tb> Growth <SEP> in <SEP> 0.1 <SEP>% <SEP> of <SEP> milk <SEP> of
<tb> blue <SEP> of <SEP> methylene
<tb><SEP> VP Reaction
<tb> Generation <SEP> of acids
<tb> Xylose
<tb> Rhamnose <SEP> +
<tb> Sucrose <SEP> +
<tb> Lactose <SEP> +
<tb> Melibiose <SEP> +
<tb> Raffinose <SEP> +
<Tb>

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<Tb>
<tb> Melezitosis <SEP> * 1
<tb> Glycerol
<tb> Adonitol <SEP> * 2
<tb> Sorbitol <SEP> +
<tb> Mannitol <SEP> +
<tb> L-arabinose
<tb> Generation <SEP> of <SEP> dye <SEP> yellow
<Tb>

Teneur par GC (% en mole) d'ADN dans le mycobion (L. 40 *1 Propriété atypique *2 Selon le procédé HPLC
Candida est une levure appartenant aux fungi imperfect. Candida lipolytica est isolée en tant que bactérie causant la détérioration du beurre, de la margarine ou équivalent en raison de la lipase qu'elle contient. On connaît de plus certains cas dans lesquels Candida lipolytica a pu être isolé à partir de l'olive, de la terre et des animaux, y compris des humains.

Content by GC (mol%) of DNA in the mycobion (L. 40 * 1 Atypical property * 2 According to the HPLC method
Candida is a yeast belonging to the imperfect fungi. Candida lipolytica is isolated as a bacterium causing the deterioration of butter, margarine or the like because of the lipase it contains. There are also some cases in which Candida lipolytica has been isolated from olive, earth and animals, including humans.

 It is preferred to use Candida lipolytica which has been deposited by the applicant internationally as DDD-c (FERM accession number BP-6465) with IPOD (International Patent Organism Depositary), AIST (National Institute Advanced Industrial Science and Technology), Japan, under the Bucharest Treaty.

The mycological properties of DDD-c of Candida lipolytica will be explained later.

<Tb>
<Tb>

ELEMENT <SEP> TEST <SEP> RESULT <SEP> OF <SEP> TEST
<tb> Morphology <SEP> of <SEP> trophocyte <SEP> form <SEP> ovoid-form
<tb> elliptical-shaped <SEP> cylindrical
<tb> Type <SEP> of <SEP> multiplication <SEP> Budding
<tb> multipolar
<tb> Culture <SEP> liquid <SEP> Precipitation <SEP> and <SEP> embedding <SEP> are
<tb> observed <SEP> (25 <SEP> C, <SEP> 3 <SEP> days)
<tb> Pseudomycelium <SEP> observed <SEP> (25 <SEP> C, <SEP> 3 <SEP> days)
<tb> Tubular <SEP><SEP> observed <SEP> (25 <SEP> C, <SEP> 3 <SEP> days) <SEP> *
<tb> Ascospore <SEP> no <SEP> observed <SEP> in <SEP><SEP> backgrounds
<tb> respective <SEP> of <SEP> Adams,
<tb> Gorodkowa, <SEP> malt, <SEP> YM, <SEP> V-8
<Tb>

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<Tb>
<tb> and <SEP> dextrose <SEP> of <SEP> apple <SEP> of
<tb> earth
<tb> Fermentation
<tb> Glucose
<tb> Galactose
<tb> Sucrose
<tb> Maltose
<tb> Lactose
<tb> Raffinose
<tb> Assimilation
<tb> Galactose
<tb> Sucrose
<tb> Maltose
<tb> Cellobiose
<tb> TrehaloseLactose
<tb> Melibiose
<tb> Raffinose
<tb> Melezitosis
<tb> Starch
<tb> D-xylose
<tb> L-arabinose
<tb> D-ribose <SEP> +
<tb> L-rhamnose
<tb> Glycerol <SEP> +
<tb> Erythritol <SEP> +
<tb> Ribitol
<tb> D-mannitol <SEP> +
<tb> Lactate <SEP> +
<tb> Succinate <SEP> +
<tb> Citrate <SEP> +
<tb> Inositol
<tb> Assimilation <SEP> of <SEP> nitrate
<tb> Growth <SEP> to <SEP> 37 <SEP> C
<tb> Growth <SEP> in <SEP> medium <SEP> lacking <SEP> of <SEP> vitamin
<Tb>

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<Tb>
<tb> Decomposition <SEP> of <SEP> Urea <SEP> mild
<tb> Coloring <SEP> of <SEP> DBB
<tb> Lipase <SEP> +
<Tb>

The three strains of microorganisms are well known in all types of normal nutrient media. In particular, MRS medium is suitable for Lactobacillus paracasei subsp. paracasei and Enterococcus malodoratus, and YM medium is suitable for Candida lipolytica.

 A preferable culture condition for the good growth of any of the foregoing microorganisms is in the range of 15 to 45 C.

 Preferably, the three microorganism strains are fixedly supported on a carrier or formed into a batch of microorganisms held in a carrier to facilitate handling of the stain repellent of the present invention.

 Each of the three strains of microorganisms may be contained in the batch of microorganisms maintained on a support in any ratio to the amount of the three strains of microorganisms, because when the strains of microorganisms are put together in water, each of them is stabilized in an approximately constant ratio, depending on the conditions of conservation or culture.

However, the function of preventing or eliminating stains and the unpleasant smell of an evacuation system can be significantly improved by appropriate disposal of the storage or cultivation conditions to obtain the following ratio:

<Tb>
<tb> Lactobacillus <SEP> paracasei <SEP> subsp. <SEP> paracasei <SEP>: <SEP> 20-60 <SEP>%
<tb> Enterococcus <SEP> Malodoratus <SEP>: <SEP> 20-60 <SEP>%
<tb> Candida <SEP> lipolytica <SEP>: <SEP> 10-30 <SEP>%.
<Tb>

 Preferably, the support for stably maintaining the microorganisms has a high retention capacity of microorganisms and an ability to facilitate the activation of microorganisms.

 Suitable support may include rocks (eg, perlite, diatomaceous earth) or trituration thereof, or broken rock, gravel, sand, plastic, ceramic (eg, alumina , silica, natural zeolite and synthetic zeolite), and talc, and in particular a porous material having pores

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 continuous, such as a porous ceramic or a porous plastic. For example, Perlite (trade name), or a fine powder, prepared by subjecting a broken perlite to heat treatment at elevated temperature and under high pressure, and then greatly reducing the pressure to impart porosity to the fractionated perlite, can be used as a suitable carrier because it has the property of allowing the microorganisms to adhere to it and grow there. Preferably, such a porous material has a pore diameter of between 2 and 10 μm. The support may be any suitable form of support such as an agglomerate, particles, powder, a fine powder, a plate and an acicular shape. The support preferably has a mean particle diameter of 2 mm or less, more preferably in the range of about 50 μm to 1 mm. The batch of microorganisms maintained on a support can be used by being contained in a container made of a fabric, a net or equivalent, which has a good permeability to air or water.

 In addition, a ribbon or woven or nonwoven sheet-like fabric may be used as a carrier.

 The microorganisms can be maintained on the support by mixing the support in a liquid dispersion in which the microorganisms of the present invention are dispersed and then drying. As an alternative, the microorganisms can be grown directly on the support.

 If the appropriate amount of the microorganisms to be maintained on the support varies depending on the conditions of the holding operation, it is preferably in the range of 5 to 20 trillion microorganisms / cm3, more preferably ranging from 10 to 10 trillion microorganisms / cm3.

 The anti-stain agent of the present invention may be incorporated into a device as described in the previous Japanese Patent No. 3150299.

 This device is formed of a woven fabric prepared by weaving fibers, yarns or equivalent of synthetic polyvinyl alcohol fibers, protein fibers (natural fibers such as wool or silk, or artificial protein fibers using casein, soy protein or equivalent), synthetic polyurethane fibers, synthetic fibers

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 polyamide or equivalent. The device may also be formed of a nonwoven fabric as described above. In the case where the device is formed into a plate shape or a ball shape, the nonwoven or woven fabric is used to cover other components so as to serve as the outermost layer of the device.

 Preferably, a plastic material is used as a hydrophilic porous plate-like material, which is an alternative material for forming the aforementioned device. The plastic material comprises a synthetic polyvinyl alcohol fiber, a synthetic polyamide fiber and a nonwoven fabric thereof. To facilitate the proliferation of microorganisms, a carbonized material such as charcoal or bamboo charcoal, microorganism activators or a combination thereof may be contained in the porous plate material. Typically, the plate-shaped material is designed to have a width in the range of about 8 to 12 mm, a thickness in the range of 1.5 to 2.0, and a length of 300. mm. A bamboo rod is used to impart strength and resilience to the plate-like device. The plate-like device is made by assembling one or more of these materials and placing the assembly in the woven fabric. The size of the plate-like device can be varied depending on the desired use and other factors. With regard to the flexibility of use, the device is preferably formed as a rectangular column whose sidesize is in the range of about 12 to 15 mm and the length in the range of about 300 to 350 mm.

 Preferably, a spherical container is used for the ball type device. The spherical container is a molded product made of a plastic material such as polystyrene, ABS resin, melamine resin, vinyl chloride resin, polypropylene, or polycarbonate, and is provided with an opening . Typically, the spherical container consists of a pair of hollow (domed) hemispherical elements each having a suitable aperture.

 The ball-type device is made by preparing the pair of dome elements, and binding together the elements to give them a

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 spherical shape. When the container is small, before the bonding operation, the aforementioned hydrophilic porous material in the form of a plate can be spirally wound and placed in the dome-shaped elements, and / or other materials can if necessary be contained in . On the other hand, when the container is large, the inner space of the dome-shaped hollow members and having the openings is preferably separated by partition walls to define a plurality of spaces including a cylindrical space in the center of the container. the internal space.

 The size and content of the ball device are approximately determined by considering the intended purpose (eg, a family use, a school, a railway station). For example, in a small device, having a diameter of about 50 mm, one or more plate-like hydrophilic porous materials are (are) typically spirally wound and placed at the same time. interior as described above. In a large device, having a diameter of about 100 mm, the above-mentioned plate-like hydrophilic porous material is interposed at the center of the device. On the lower and upper sides of the plate-shaped hydrophilic porous material, a plurality of small, typically 4- to 8-membered balls (each preferably having an opening) formed of a hydrophilic porous material and containing a coal on which microorganisms are maintained and / or a hydrophilic porous material containing a microorganism activating agent on which aerobic microorganisms are maintained are respectively placed in the separate spaces.

In addition, a hydrophilic porous material on which ceramic particles and microorganisms are held may conveniently be placed in the central space.

 To allow the microorganisms to be maintained on each of the materials constituting these devices, one can use as such a method described in the aforementioned patent application. Specifically: (1) Preparation of a polyvinyl alcohol stock solution: divalent and / or trivalent iron salts are added in purified water in an amount of 10-10 to 10-12% by volume then a lean coal pellet (Quercus charcoal phillyraeoides) is added to the liquid. Then we

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 adding a polyvinyl alcohol powder in an amount equal to 5 to 15% by weight, preferably about 10% by weight, in the liquid, while heating gently. The liquid is stirred and heated to 75 ° C. and then allowed to cool. Preferably, the divalent or trivalent salts such as iron chloride or iron sulfate are used as iron salts.

 (2) Preparation of the microorganism culture medium: salts including various purified minerals are added from seawater (eg potassium salts, sodium salts, magnesium salts) in a tank containing tap water, and mixed to give an NaCl concentration of between 0.7 and 1.2%, preferably between 0.9 and 1.0%. The vessel is provided with an air circulating pump for aerating and a submerged pump having rotary valves, a pipe for fluid communication between the two, and an automatically controlled heater for adjusting a temperature of the water. It is desirable to use the submerged pump at a speed of 100 revolutions per minute or more, and with a flow efficiency of 95 to 97% (theoretical standard). This allows a very efficient stirring of the water in the tank. The temperature of the water is typically controlled to be in the range of 23 to 30 ° C, preferably in the range of 25 to 27 ° C.

 (3) A saponin-containing plant-derived surfactant as a microorganism activating agent (trade name: Econin, manufactured by Tashirokogyo Co.) is added to the vessel in an amount of 1 x 10-2 and 1 x 10-5% by volume, preferably between 5 x 10-3 and 1 x 10-4% by volume. Saponin has a surfactant effect, and it is known in particular that the triterpenoid saponin contained in Econin has an effect of improving the reaction of a microorganism, suppressing stress on microorganisms and improving the elimination of fats. by micro-organisms, as biological activities. Econin also includes a saccharide, ethanol and water. Coal material is added to the vessel to impart an improved growth condition to the microorganisms. The coal contains minor elements necessary for the growth of microorganisms. Specifically, charcoal contains 2 to 3% inorganic ash and a large amount of potassium, calcium and

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 magnesium, and bamboo charcoal or straw charcoal contains about 11% inorganic ash, of which 90% or more is silicon.

In the present invention, charcoal (lean coal) and bamboo charcoal (which may include straw charcoal) are blended at a ratio of last and first named of 20-40%: 80-60%, preferably about 30: about 70%. Preferably, the carbon material is used in the form of a powder having a mean diameter in the range of 30 to 50 μm. The carbon material is added in the range of 0.1 to 1.0% by weight, preferably in the range of 0.3 to 0.5% by weight.

 (4) Next, the aforementioned microorganisms are added to the liquid. Proliferation is observed after keeping them in culture for 1 to several days. Once the proliferation of aerobic microorganisms is observed, the polyvinyl alcohol described in (1) is added to the liquid. The amount added is in the range of 0.05 to 0.1% by volume, preferably in the range of 0.07 to 0.08% by volume based on total liquid. By adding the polyvinyl alcohol, a large amount of fine bubbles (diameter in the range of 0.1 to 1.0 mm) is generated, and some viscosity is observed between the bubbles. The bubbles are minimized in the water agitation vessel to have a diameter substantially in the range of about 0.1 to 0.5 mm, and the minimized bubbles remain in the vessel. The viscous bubbles are typically maintained for 5-6 hours and then dissolved in the liquid phase over time. If an additional liquid containing polyvinyl alcohol stock solution or a salt is additionally added to the tank, the bubbles will be generated continuously.

dispositif de la présente invention est ajouté dans la cuve. Le matériau est i immergé dans la cuve pendant 12 à 24 heures. Ensuite, le matériau est retiré de la cuve et séché à l'air pour entraîner l'oxydation sur la surface de celui-ci. L'opération de séchage est effectuée à une température comprise dans la gamme allant de 20 à 30 C, de préférence à environ 25 C, pendant environ 6 à 12 heures. Un revêtement multicouches sera formé sur la surface du matériau en répétant les opérations précédentes environ 1 à (5) Preparation of the respective materials maintaining the microorganisms: in the previous state, each of the materials constituting the

device of the present invention is added to the vessel. The material is immersed in the tank for 12 to 24 hours. Then, the material is removed from the tank and air dried to cause oxidation on the surface thereof. The drying operation is carried out at a temperature in the range of from 20 to 30 ° C, preferably at about 25 ° C, for about 6 to 12 hours. A multilayer coating will be formed on the surface of the material by repeating the preceding operations about 1 to

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 4 times, preferably about 2 to 3 times. While the thickness of a single layer varies depending on the concentration of the polyvinyl alcohol or the immersion time in the tank, the single layer is typically a thin film having a thickness within the range ranging from 5 to 10 lam. The fine particulate coal material exists between the material surface and the coating and between the respective layers to reliably provide a flow path for dissolved oxygen, water and microorganisms. In this way, the targeted microorganisms can be impregnated into or attached to each of the materials. In another embodiment, the carbon material and the microorganism activating agent are suitably impregnated in advance in each of the materials by an appropriate method, and the material is then immersed in a vessel containing the micro-organisms. -organisms and polyvinyl alcohol to allow microorganisms to be maintained on the material.

 By using the materials that maintain the microorganisms by the aforementioned method, the device can be assembled into a plate or a ball. In the plate-like device or in the small ball-like device, the woven fabric forms the outermost layer of each of the materials.

 When the device is used in a toilet bowl, the location of the device is important. The plate-like device is disposed immediately directly below the flush outlet of a urinal. On the other hand, the ball-type device is used inside a flush water tank of a urinal. By thus arranging the devices, a portion of the microorganisms can be mixed in the flush water discharged after using the urinal to effectively remove urine stains and the like. More specifically, a stain-causing material at a stained portion of the urinal is gradually decomposed and removed by the microorganisms at the stained portion. Even if a part of the microorganisms on the outer layer of the device coating is discharged, the microorganisms of the second layer and the other underlying layers will automatically fill the unloaded microorganisms as the outermost layer is in communication with the underlying layers. In addition, the properties of water and other circumstances associated with microorganisms

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 are regulated by the carbon material placed in the materials constituting the device, and thus, the microorganisms can continue to grow using some of the materials as a source of nutrients.

As a result, the effects of the device can be stably maintained for a long time, typically for one year after deposition of the device at a given location.

 The functions of the device are not affected by sodium hypochlorite from tap water. Even if the activity of the microorganisms on the outermost layer of the device is temporarily damaged by applying a disinfectant or equivalent, the original activity can be recovered within a few weeks because the microorganisms of the second layer and other layers Underlying events come to the surface when the effect becomes weaker, as previously described. When the device is not used for a certain time, for example more than six months, the activity can be maintained by storing the device in a dark place without sealing it hermetically.

 The three strains of microorganisms can interact with one another to effectively break down organic substances and mineral deposits in an evacuation system, particularly in the toilet bowl and its water pipes.

 The safety of each of the three microorganism strains was verified by an oral test using mice.

 In a method of preventing or removing stains from a piping system by the use of the above-mentioned stain-repellent agent of the present invention, the microorganisms of the stain-repellent agent of the invention are brought into contact with or dispersed in the water in the drainage system in an appropriate manner. As a result, the purified evacuation obtained itself and the microorganisms in the discharge liquid (or feed water) allow the unpleasant stains and odors of the exhaust system to be eliminated or avoided.

 For example, when an evacuation system includes a toilet bowl and a flush pipe of a flush system, the stain remover is disposed in the supply tank of the supply system. in water. In this way, the microorganisms are grown in the feedwater, and the water containing microorganisms

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 well grown is brought into the evacuation system. This allows for effective removal or prevention of unpleasant stains and odors.

 The evacuation system of the present invention is not limited to the above-mentioned toilet system, and the present invention can be effectively applied to other suitable disposal systems including calcium or organic materials in the system. evacuation, such as an evacuation system from a factory or building, or an evacuation system from households' kitchens or bathrooms.

 An example of the present invention will be described later.

We begin by preparing a medium having the following composition: MIDDLE

<Tb>
<tb> Peptone <SEP> 5g
<tb> Extracts <SEP> of <SEP> meat <SEP> 5 <SEP> g
<tb> NaCl <SEP> 2, <SEP> 5 <SEP> g
<tb> Water <SEP> 500 <SEP> ml <SEP> (pH <SEP> 7.0)
<Tb>

The medium having the aforementioned composition is placed in a 1000 ml container. The DDD-a, DDD-b and DDD-c microorganisms deposited with the IPOD (International Patent Organism Depositary) are then cultured in the medium. The solution is shaken vigorously and placed in culture for 1 day at 30 ° C. The ratio of DDD-α, DDD-b and DDD-c microorganisms is about 40:40:20%.

3 maintenus sur le support est d'environ 5 millions de micro-organismes/cm3. The resulting culture solution is subjected to a centrifugal separator to isolate the microorganisms. The isolated microorganisms are thoroughly washed and held on a support to give the stain-inhibiting agent in the form of a powder. The support is prepared by heating and spraying a diatomaceous earth. The quantity of microorganisms

3 maintained on the support is about 5 million microorganisms / cm3.

 100 grams of the powder stain repellent is placed in a bag made of a fine textured fabric, and the bag is immersed with the agent in the urinal flush tank (4 urinals arranged in line) The tank has a volume of 10 liters and automatically feeds the flushing water containing the above-mentioned microorganisms to the urinal after use of the urinal.

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The hunting frequency varies according to each urinal in the range from approximately every 3 minutes to every hour.

 As a result, after 20 days, stains such as yellowish and brownish stains can be removed by a simple toilet brush without the use of a cleaner. After 30 days, the mineral deposit attached around the evacuation hole in the lower portion of the urinal begins to dissolve. After 45 days, the mineral deposit can be largely eliminated by rubbing hard with a toilet brush.

Then, after 6 months, there is no trace of stain, and the previously unpleasant odor has disappeared.

 The advantageous effects of the present invention become apparent from the previous example.

Claims (16)

An anti-stain agent for an evacuation system, containing: at least one strain of microorganisms selected from the group consisting of Lactobacillus paracasei subsp. paracasei and Enterococcus malodoratus; and Candida lipolytica.  2. An anti-stain agent as defined in claim 1, wherein said strains are maintained on a suitable support for immersing said strains in water.  An anti-stain agent as defined in claim 1 or 2, wherein said evacuation system comprises a toilet bowl and an exhaust pipe of a flush toilet system.  4. Evacuation system anti-stain agent, containing three strains of microorganisms composed of Lactobacillus paracasei subsp. paracasei and Enterococcus malodoratus, and Candida lipolytica.  An anti-stain agent as defined in claim 4, wherein each of said three strains of microorganisms has the following ratio with respect to the total amount of the three strains of microorganisms:

<Tb> <tb> Candida <SEP> lipolytica <SEP>: <SEP> 10-30 <SEP>%. <tb> Enterococcus <SEP> Malodoratus <SEP>: <SEP> 20-60 <SEP>% <tb> Lactobacillus <SEP> paracasei <SEP> subsp. <SEP> paracasei <SEP>: <SEP> 20-60 <SEP>% <Tb>  An anti-stain agent as defined in claim 4 or 5, wherein said microorganisms are held on a suitable support for immersing said strains in water.  An anti-stain agent as defined in one of claims 4 to 6, wherein said evacuation system comprises a toilet bowl and an exhaust pipe of a flush toilet system.  A method of preventing or cleaning a stain of an evacuation system, wherein an anti-stain agent containing Candida lipolytica, and at least one strain of microorganisms selected from the group consisting of Lactobacillus paracasei subsp. paracasei and Enterococcus malodoratus, is brought into contact with water of said evacuation system at one or more locations of said evacuation system so as to prevent a stain from adhering to said system <Desc / Clms Page number 20>  discharge or to decompose and remove a stain that adhered to said evacuation system.  9. The method as defined in claim 8, wherein said microorganisms are maintained on a suitable support for immersing said microorganisms in water.  The method as defined in claim 8 or 9, wherein said exhaust system comprises a toilet bowl and an exhaust pipe of a flush toilet system.  The method as defined in claim 10, wherein said microorganisms are added to water in a water supply channel of said flush toilet system to be subsequently contacted with water in said toilet bowl and said drain pipe.  12. A method of preventing or cleaning a stain of an evacuation system, wherein an anti-stain agent containing three strains of microorganisms composed of Candida lipolytica, Lactobacillus paracasei subsp. paracasei and Enterococcus malodoratus, is brought into contact with water in said drainage system at one or more locations of said drainage system so as to prevent a stain from adhering to said evacuation system or to decompose and remove a stain that adhered to said evacuation system.  13. The method as defined in claim 12, wherein said microorganisms are maintained on a suitable support for immersing said microorganisms in water.  The method as defined in claim 12 or 13, wherein said exhaust system comprises a toilet bowl and an exhaust pipe of a flush toilet system.  The method as defined in claim 14, wherein said microorganisms are added to water in a water supply channel of said flush toilet system to then be contacted with water in said toilet bowl and in said drain pipe.  16. The method as defined in one of claims 11 to 15, wherein each of the three strains of microorganisms has the <Desc / Clms Page number 21>

 next report with regard to the total quantity of the three strains of microorganisms: <Tb> <tb> Candida <SEP> lipolytica <SEP>: <SEP> 10-30 <SEP>%. <tb> Enterococcus <SEP> Malodoratus <SEP>: <SEP> 20-60 <SEP>% <tb> Lactobacillus <SEP> paracasei <SEP> subsp. <SEP> paracasei <SEP>: <SEP> 20-60 <SEP>% <Tb>