FR2665447B1 - Procede de purification d'une proteine presente dans des corps d'inclusion. - Google Patents

Procede de purification d'une proteine presente dans des corps d'inclusion.

Info

Publication number
FR2665447B1
FR2665447B1 FR9009829A FR9009829A FR2665447B1 FR 2665447 B1 FR2665447 B1 FR 2665447B1 FR 9009829 A FR9009829 A FR 9009829A FR 9009829 A FR9009829 A FR 9009829A FR 2665447 B1 FR2665447 B1 FR 2665447B1
Authority
FR
France
Prior art keywords
inclusion
purification
bodies
protein present
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9009829A
Other languages
English (en)
Other versions
FR2665447A1 (fr
Inventor
Paul Francois
Bernard
Auriol Daniel
Henri
Monsan Pierre
Frederic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bioeurope
Original Assignee
Bioeurope
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioeurope filed Critical Bioeurope
Priority to FR9009829A priority Critical patent/FR2665447B1/fr
Priority to KR1019920700731A priority patent/KR920702414A/ko
Priority to JP3513932A priority patent/JPH05502795A/ja
Priority to PCT/FR1991/000629 priority patent/WO1992002540A1/fr
Priority to EP91914326A priority patent/EP0495059A1/fr
Priority to CA002066598A priority patent/CA2066598A1/fr
Publication of FR2665447A1 publication Critical patent/FR2665447A1/fr
Application granted granted Critical
Publication of FR2665447B1 publication Critical patent/FR2665447B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
FR9009829A 1990-08-01 1990-08-01 Procede de purification d'une proteine presente dans des corps d'inclusion. Expired - Fee Related FR2665447B1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
FR9009829A FR2665447B1 (fr) 1990-08-01 1990-08-01 Procede de purification d'une proteine presente dans des corps d'inclusion.
KR1019920700731A KR920702414A (ko) 1990-08-01 1991-07-30 봉입체내에 존재하는 단백질 정제방법
JP3513932A JPH05502795A (ja) 1990-08-01 1991-07-30 封入体中に存在するタンパク質の精製方法
PCT/FR1991/000629 WO1992002540A1 (fr) 1990-08-01 1991-07-30 Procede de purification d'une proteine presente dans des corps d'inclusion
EP91914326A EP0495059A1 (fr) 1990-08-01 1991-07-30 Procede de purification d'une proteine presente dans des corps d'inclusion
CA002066598A CA2066598A1 (fr) 1990-08-01 1991-07-30 Procede de purification d'une proteine presente dans des corps d'inclusion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9009829A FR2665447B1 (fr) 1990-08-01 1990-08-01 Procede de purification d'une proteine presente dans des corps d'inclusion.

Publications (2)

Publication Number Publication Date
FR2665447A1 FR2665447A1 (fr) 1992-02-07
FR2665447B1 true FR2665447B1 (fr) 1992-12-04

Family

ID=9399312

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9009829A Expired - Fee Related FR2665447B1 (fr) 1990-08-01 1990-08-01 Procede de purification d'une proteine presente dans des corps d'inclusion.

Country Status (6)

Country Link
EP (1) EP0495059A1 (fr)
JP (1) JPH05502795A (fr)
KR (1) KR920702414A (fr)
CA (1) CA2066598A1 (fr)
FR (1) FR2665447B1 (fr)
WO (1) WO1992002540A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2383392A (en) * 1992-07-21 1994-02-14 Immunex Corporation Process for isolating recombinant polypeptides
CH701129A2 (fr) 2009-05-29 2010-11-30 Fond The Ark Procédé de purification et de renaturation d'une protéine recombinante.
ES2645634T3 (es) 2013-02-12 2017-12-07 Bristol-Myers Squibb Company Métodos de replegado de proteínas a elevado pH
EP3744728A1 (fr) 2013-02-12 2020-12-02 Bristol-Myers Squibb Company Procédés de repliement de protéine utilisant la filtration tangentielle

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0678360B2 (ja) * 1987-08-03 1994-10-05 太陽化学株式会社 卵黄リポタンパク質と卵黄水溶性タンパク質の分画方法

Also Published As

Publication number Publication date
JPH05502795A (ja) 1993-05-20
KR920702414A (ko) 1992-09-04
EP0495059A1 (fr) 1992-07-22
CA2066598A1 (fr) 1992-02-02
FR2665447A1 (fr) 1992-02-07
WO1992002540A1 (fr) 1992-02-20

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Legal Events

Date Code Title Description
ST Notification of lapse