FR2595247A1 - Use in cosmetology of extracts of mucus of or digestive juice of gastropods and preparation of these extracts - Google Patents

Use in cosmetology of extracts of mucus of or digestive juice of gastropods and preparation of these extracts Download PDF

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Publication number
FR2595247A1
FR2595247A1 FR8603135A FR8603135A FR2595247A1 FR 2595247 A1 FR2595247 A1 FR 2595247A1 FR 8603135 A FR8603135 A FR 8603135A FR 8603135 A FR8603135 A FR 8603135A FR 2595247 A1 FR2595247 A1 FR 2595247A1
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France
Prior art keywords
extracts
mucus
gastropods
digestive juice
cosmetology
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FR8603135A
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French (fr)
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FR2595247B1 (en
Inventor
Daniel Greff
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Sederma SA
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Sederma SA
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Priority to FR8603135A priority Critical patent/FR2595247B1/en
Publication of FR2595247A1 publication Critical patent/FR2595247A1/en
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Publication of FR2595247B1 publication Critical patent/FR2595247B1/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Zoology (AREA)
  • Cosmetics (AREA)

Abstract

The invention relates to the use in cosmetic products of extracts of mucus or of digestive juice of gastropods. These extracts chiefly contain native or partially hydrolysed glycoproteins. These extracts are prepared according to a method employing a combination of a series of filtrations, an exclusion chromatographic procedure and, where appropriate, a partial enzymatic hydrolysis. The end products contain 0.1 to 10 % of these extracts.

Description

UTILISATION EN COSMETOLOGIE D'EXTRAITS DE MUCUS OU DE SUC DIGESTIF DE
GASTEROPODES ET PREPARATION DE CES EXTRAITS.USE IN COSMETOLOGY OF MUCUS EXTRACTS OR DIGESTIVE SUGAR
GASTEROPODS AND PREPARATION OF THESE EXTRACTS.

Les substances possédant une action hydratante à la surface de l'epi- derme presentent un très grand intérêt pour le cosmétologue.Substances with a hydrating action on the surface of the epidermis are of great interest to the cosmetologist.

Parmi ces substances figurent en très bonne place les glycoprotéines qui possèdent une capacité de rétention d'eau élevée due principalement à la présence de leurs résidus glycosylés.Among these substances are prominently glycoproteins which have a high water retention capacity mainly due to the presence of their glycosylated residues.

Les glycoproteines se rencontrent chez pratiquement tous les êtres vivants.Glycoproteins are found in almost all living things.

Elles sont le plus souvent secrétées dans les milieux extracellulaires où elles contribuent à maintenir une teneur en eau élevée.They are most often secreted in extracellular environments where they help maintain a high water content.

Des glycoproteines sont présentes en tres grandes quantités, sous la forme d'enzymes variées (protéases, estérases, sulfatases, glycosidases...) dans le suc digestif des gastéropodes. Elles représentent également le constituant essentiel du mucus des gastéropodes terrestres.Glycoproteins are present in very large quantities, in the form of various enzymes (proteases, esterases, sulfatases, glycosidases ...) in the digestive juice of gastropods. They also represent the essential constituent of the mucus of terrestrial gastropods.

Le mucus et le suc digestif des gastéropodes récoltés des fins alimentaires, tels que l'escargot de Bourgogne (Helix pomatia) constituent donc une source abondante de glycoproteines présentant un grand intérêt en cosmétologie.The mucus and digestive juice of gastropods harvested for food, such as the Burgundy snail (Helix pomatia) therefore constitute an abundant source of glycoproteins of great interest in cosmetology.

La méthode de la présente invention permet de réaliser l'extraction et la purification de ces glycoproteines à l'échelle industrielle. Elle comporte les phases suivantes - Le mélange de mucus et de sucs digestifs d'escargots est récupéré après
le "dégorgement" des animaux. Il est' immédiatement congele pour le
transport et le stockage.The method of the present invention allows the extraction and purification of these glycoproteins on an industrial scale. It includes the following phases - The mixture of mucus and digestive juices of snails is recovered after
the "disgorgement" of animals. It is immediately frozen for
transport and storage.

- Ce produit brut est mis à decongeler en le diluant de moitié dans de
l'eau déminéralisée. La solution ainsi obtenue est soumise à une série
de filtrations en cascade destinees à en éliminer les impuretes. Pour
finir, on-réalise une filtration stérilisante sur une cartouche filtrante
stérile possédant des pores d'un diamètre nominal de 0,22 micromètres.- This raw product is thawed by diluting it by half in
demineralized water. The solution thus obtained is subjected to a series
cascade filtration intended to remove impurities. For
finish, we perform a sterilizing filtration on a filter cartridge
sterile with pores with a nominal diameter of 0.22 micrometers.

- Les glycoproteines contenues dans le filtrat sont alors purifiees par
chromatographie d'exclusion sur un gel de type Ultragel AcA 202 (LIB),
l'élution étant réalisée au moyen de tampon phosphate 0,01 M, pH 6,5.- The glycoproteins contained in the filtrate are then purified by
exclusion chromatography on an Ultragel AcA 202 (LIB) gel,
the elution being carried out using 0.01 M phosphate buffer, pH 6.5.

- La taille des glycoproteines purifiées ainsi obtenues peut éventuellement
être réduite au moyen d'une protéolyse enzymatique modérée qui peut être
effectuée avec des proteases courantes : trypsine, chymotrypsine,
pepsine, papaine;... - The size of the purified glycoproteins thus obtained can possibly
be reduced by moderate enzymatic proteolysis which can be
performed with common proteases: trypsin, chymotrypsin,
pepsin, papain; ...

- La réaction enzymatique est arrêtee au moyen d'un inhibiteur spécifique
de la protéase utilise.- The enzymatic reaction is stopped by means of a specific inhibitor
of the protease uses.

- Le produit ainsi obtenu possède un pouvoir hydratant très élevé. Il peut
être inclus aux concentrations de 0,1 à 10 r dans des formulations
cosmétiques. - The product thus obtained has a very high hydrating power. he can
be included in concentrations of 0.1 to 10 r in formulations
cosmetics.

Un exemple (non limitatif) de formule est donné ci-dessous - stéarate de glycérol AE 3 S - polysorbate 60 3 % - stéarate de sorbitan 3 % - huile minérale 10 , - octyl-dodecanol 3 ze - myristate d'isopropyl 2 , - sorbitol 3 g - paraoxybenzoate de méthyle 0,3 % - parfum Chemoderm 981 0,3 r - EXTRAIT DE MUCUS D'ESCARGOTS 8 r - eau déminéralisée q.s.p. 100 An example (non-limiting) of the formula is given below - glycerol stearate AE 3 S - polysorbate 60 3% - sorbitan stearate 3% - mineral oil 10, - octyl-dodecanol 3 ze - isopropyl myristate 2, - sorbitol 3 g - methyl paraoxybenzoate 0.3% - Chemoderm 981 fragrance 0.3 r - SUCK MUCUS EXTRACT 8 r - demineralized water qs 100

Claims (4)

natives ou partieTlement hydrolysées. native or partially hydrolyzed. gastéropodes caractérisés en ce qu'ils renferment des glycoprotéines  gastropods characterized in that they contain glycoproteins REVENDICATIONS 1.Utilisation en cosmétique d'extraits de mucus ou de suc digestif de CLAIMS 1. Use in cosmetics of mucus extracts or digestive juice of 2.Procédé de préparation d'extraits de mucus ou de suc digestif de2. Process for preparing mucus extracts or digestive juice gastéropodes selon la revendication 1, caractérisé en ce qu'une gastropods according to claim 1, characterized in that chromatographie d'exclusion est réalisée pour purifier le produit. exclusion chromatography is performed to purify the product. 3.Procédé de preparation d'extraits de mucus ou de suc digestif de3. Process for preparing mucus extracts or digestive juice gastéropodes selon l'une quelconque des revendications 1 et 2, gastropods according to any one of claims 1 and 2, caractérisé en ce qu'une hydrolyse enzymatique est effectuée sur characterized in that an enzymatic hydrolysis is carried out on le produit. the product. 4.Préparations cosmétiques caractérisées n ce qu'elles contiennent4.Characterized cosmetic preparations n what they contain de 0,1 à 10 % d'extraits de mucus ou de suc digestif de gastéropodes 0.1 to 10% extract of mucus or gastric digestive juice selon l'une quelconque des revendications 1 à 3.  according to any one of claims 1 to 3.
FR8603135A 1986-03-06 1986-03-06 USE IN COSMETOLOGY OF MUCUS EXTRACTS OR DIGESTIVE SUGAS OF GASTEROPODS AND PREPARATION OF SUCH EXTRACTS Expired FR2595247B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR8603135A FR2595247B1 (en) 1986-03-06 1986-03-06 USE IN COSMETOLOGY OF MUCUS EXTRACTS OR DIGESTIVE SUGAS OF GASTEROPODS AND PREPARATION OF SUCH EXTRACTS

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8603135A FR2595247B1 (en) 1986-03-06 1986-03-06 USE IN COSMETOLOGY OF MUCUS EXTRACTS OR DIGESTIVE SUGAS OF GASTEROPODS AND PREPARATION OF SUCH EXTRACTS

Publications (2)

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FR2595247A1 true FR2595247A1 (en) 1987-09-11
FR2595247B1 FR2595247B1 (en) 1988-09-23

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992015276A2 (en) * 1991-03-01 1992-09-17 Mcginnis, Michael Therapeutic and cosmetic compositions for treatment of skin
WO1995015774A1 (en) * 1993-12-10 1995-06-15 Camprasse Georges Injectable implant for correcting wrinkles and skin hollows
US5538740A (en) * 1991-03-01 1996-07-23 Atherton Investments, Ltd. Therapeutic and cosmetic compositions for treatment of skin
FR2866238A1 (en) * 2004-02-13 2005-08-19 Innovation Et De Rech Applique Extrapalladial fluid recovered from between shell and body of molluscs, e.g. oysters, useful as medicament or food supplement having e.g. cicatrization, osteogenesis and bone mineralization promoting action
WO2014085876A1 (en) * 2012-12-03 2014-06-12 "Cantareus" Jsc Cosmetic composition
US10391050B2 (en) 2010-01-28 2019-08-27 Industrial Farmacéutica Cantabria, S.A. Dermatological, cosmetic or cosmeceutical compositions intended for skin treatment
WO2022100807A1 (en) 2020-11-11 2022-05-19 Bioresources Technology & Engineering Gmbh (Bite) Mucus from leeches and use in cosmetic preparations
WO2022160425A1 (en) * 2021-02-01 2022-08-04 大连海洋大学 Anti-hepatoma and anti-liver fibrosis oligopeptide extracted from marine gastropod mucus

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3664M (en) * 1963-08-30 1965-11-08 Erchim S A
GB1166939A (en) * 1966-09-29 1969-10-15 Prephar Prospection De Rech S Process For The Extraction Of A Glycopeptide
DE1813154A1 (en) * 1968-12-06 1970-07-02 Helmut Seuss Skin cream containing snail secretion
EP0149872A2 (en) * 1984-01-09 1985-07-31 Crinos Industria Farmacobiologica S.p.A. Cosmetic composition containing glycoproteins for the skin care

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3664M (en) * 1963-08-30 1965-11-08 Erchim S A
GB1166939A (en) * 1966-09-29 1969-10-15 Prephar Prospection De Rech S Process For The Extraction Of A Glycopeptide
DE1813154A1 (en) * 1968-12-06 1970-07-02 Helmut Seuss Skin cream containing snail secretion
EP0149872A2 (en) * 1984-01-09 1985-07-31 Crinos Industria Farmacobiologica S.p.A. Cosmetic composition containing glycoproteins for the skin care

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 104, 1986, page 357, résumé no. 32013p, Columbus, Ohio, US; Y. KUBOTA et al.: "Purification and characterization of an antibacterial factor from snail mucus", & COMP. BIOCHEM. PHYSIOL., C: COMP. PHARMACOL. TOXICOL. 1985, 82C(2), 345-8 *
CHEMISCHES ZENTRALBLATT, no. 25, 1962, page 9182; G. BRAMBILLA et al.: "Erste Versuche }ber die Verwendung von Extrakten aus Helix Pomatia L. und verwandten Arten in der Kosmetik" *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992015276A2 (en) * 1991-03-01 1992-09-17 Mcginnis, Michael Therapeutic and cosmetic compositions for treatment of skin
WO1992015276A3 (en) * 1991-03-01 1992-11-12 Mcginnis Michael Therapeutic and cosmetic compositions for treatment of skin
US5538740A (en) * 1991-03-01 1996-07-23 Atherton Investments, Ltd. Therapeutic and cosmetic compositions for treatment of skin
WO1995015774A1 (en) * 1993-12-10 1995-06-15 Camprasse Georges Injectable implant for correcting wrinkles and skin hollows
FR2713482A1 (en) * 1993-12-10 1995-06-16 Camprasse Georges Injectable implant for the correction of wrinkles and dermal depressions.
FR2866238A1 (en) * 2004-02-13 2005-08-19 Innovation Et De Rech Applique Extrapalladial fluid recovered from between shell and body of molluscs, e.g. oysters, useful as medicament or food supplement having e.g. cicatrization, osteogenesis and bone mineralization promoting action
WO2005077388A1 (en) * 2004-02-13 2005-08-25 Societe D'innovation Et De Recherche Appliquee Sa (Siera Sa) Extrapallial fluid of molluscs, method of obtaining same, formulation and use thereof
US10391050B2 (en) 2010-01-28 2019-08-27 Industrial Farmacéutica Cantabria, S.A. Dermatological, cosmetic or cosmeceutical compositions intended for skin treatment
WO2014085876A1 (en) * 2012-12-03 2014-06-12 "Cantareus" Jsc Cosmetic composition
WO2022100807A1 (en) 2020-11-11 2022-05-19 Bioresources Technology & Engineering Gmbh (Bite) Mucus from leeches and use in cosmetic preparations
WO2022160425A1 (en) * 2021-02-01 2022-08-04 大连海洋大学 Anti-hepatoma and anti-liver fibrosis oligopeptide extracted from marine gastropod mucus

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Publication number Publication date
FR2595247B1 (en) 1988-09-23

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