FR2552674A1 - Process for improving the flow in the ultrafiltration of the amino acids obtained by microbial fermentation. - Google Patents
Process for improving the flow in the ultrafiltration of the amino acids obtained by microbial fermentation. Download PDFInfo
- Publication number
- FR2552674A1 FR2552674A1 FR8319862A FR8319862A FR2552674A1 FR 2552674 A1 FR2552674 A1 FR 2552674A1 FR 8319862 A FR8319862 A FR 8319862A FR 8319862 A FR8319862 A FR 8319862A FR 2552674 A1 FR2552674 A1 FR 2552674A1
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- Prior art keywords
- sep
- ultrafiltration
- liquor
- flow
- membrane
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- 238000000108 ultra-filtration Methods 0.000 title claims abstract description 23
- 238000000855 fermentation Methods 0.000 title claims abstract description 8
- 230000004151 fermentation Effects 0.000 title claims abstract description 8
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 5
- 238000000034 method Methods 0.000 title claims abstract description 4
- 230000000813 microbial effect Effects 0.000 title description 4
- 238000010438 heat treatment Methods 0.000 claims abstract description 20
- 239000012528 membrane Substances 0.000 claims abstract description 20
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 6
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 6
- 239000006227 byproduct Substances 0.000 claims abstract description 3
- 238000001914 filtration Methods 0.000 claims description 18
- 229920000642 polymer Polymers 0.000 claims description 3
- 230000004048 modification Effects 0.000 abstract description 2
- 238000012986 modification Methods 0.000 abstract description 2
- 230000015271 coagulation Effects 0.000 abstract 1
- 238000005345 coagulation Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 5
- 239000012510 hollow fiber Substances 0.000 description 3
- 229920002492 poly(sulfone) Polymers 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000013379 molasses Nutrition 0.000 description 2
- 229920002239 polyacrylonitrile Polymers 0.000 description 2
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000010409 ironing Methods 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/02—Hollow fibre modules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/06—Tubular membrane modules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/08—Flat membrane modules
- B01D63/087—Single membrane modules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/10—Spiral-wound membrane modules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/08—Prevention of membrane fouling or of concentration polarisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
Abstract
Description
On sait qu'une liqueur de fermentation microbienne, par exemple d'amino-acides, est soumise directement à l'ultrafiltration pour éliminer les micro-organismes. Cependant la liqueur fermentée contient outre la substance recherchée et les micro-organismes, des polymères tels que protéines1 sucres etc. qui sont des sousproduits de la réaction microbienne. Ces polymères provoquent une obturation de la membrane lors de l'ultrafiltration et posent donc un problème parce que le flux de liqueur, c'est-a-dire la quantité passant par unité de temps et par unité de surface de la membrane, est réduit. It is known that a microbial fermentation liquor, for example amino acids, is subjected directly to ultrafiltration to eliminate microorganisms. However, the fermented liquor contains in addition to the desired substance and the micro-organisms, polymers such as proteins, sugars, etc. which are byproducts of the microbial reaction. These polymers cause a closure of the membrane during ultrafiltration and therefore pose a problem because the flow of liquor, that is to say the amount passing per unit of time and per unit area of the membrane, is reduced .
Dans ces circonstances la demanderesse s'est penchée sur le fait que les substances polymères telles que les protéines ont la propriété de se modifier et de coaguler par chauffage et a donc découvert que le problème ci-dessus peut entre résolu dans l'ultrafiltration d'une liqueur fermentée par un traitement préalable de chauffage de ladite liqueur. La présente invention repose sur cette découverte. En d'autres termes il est possible de-modifier et coaguler les substances polymères telles que les protéines comme décrit ci-dessus par chauffage et d'empocher ainsi l'obtura- tion de la membrane d'ultrafiltration et par conséquent d'augmenter- le flux,c'est-d-dire la capacité de perméation de ladite solution. In these circumstances, the Applicant has examined the fact that polymeric substances such as proteins have the property of modifying and coagulating by heating and thus discovered that the above problem can be solved in the ultrafiltration of a liquor fermented by a prior treatment for heating said liquor. The present invention is based on this discovery. In other words, it is possible to modify and coagulate the polymeric substances such as the proteins as described above by heating and to thus pocket the closure of the ultrafiltration membrane and consequently to increase the flow, that is to say the permeation capacity of said solution.
Bien que les conditions précises du traitement de chauffage varient selon le type de la liqueur d traiter, on obtient généralement satisfaction avec des températures de 50 - 1000C et des durées de plusieurs secondes a plusieurs dizaines de minutes. Although the precise conditions of the heating treatment vary according to the type of the liquor to be treated, satisfaction is generally obtained with temperatures of 50.degree.-1000.degree. C. and durations of several seconds to several tens of minutes.
En ce qui concerne l'admission au traitement d'ultrafiltration après chauffage, c'est-à-dire si elle doit être faite chaud ou si elle doit ou peut entre faite après refroidissement, ceci doit être déterminé selon les résultats de la membrane d'ultrafiltration, les propriétés de la substance recherchée, etc.With regard to the admission to ultrafiltration treatment after heating, that is to say if it must be made hot or if it must or can be done after cooling, this must be determined according to the results of the membrane of ultrafiltration, the properties of the desired substance, etc.
L'ultrafiltration proprement dite est une technique connue comme décrite ci-dessus et la liqueur d traiter qui a été soumise au prétraitement selon l'invention peut# ensuite autre soumise à ce traitement connu d'ultrafiltration. The ultrafiltration proper is a technique known as described above and the liquor to be treated which has been subjected to the pretreatment according to the invention can then be further subjected to this known ultrafiltration treatment.
La membrane d'ultrafiltration utilisée dans l'invention n'est pas particulièrement limitée et peut entre en une substance semi-perméable classique. On citera par exemple les polyamides, le polyacrylonitrile, les polysulfones, llacétate de cellulose, etc. The ultrafiltration membrane used in the invention is not particularly limited and can enter into a conventional semi-permeable substance. Examples include polyamides, polyacrylonitrile, polysulfones, cellulose acetate, and the like.
La forme de la membrane n'est pas particulièrement limitée et celle-ci peut être par exemple tubulaire, plate, en spirale, sous forme de fibrescreuses,etc. Le poids moléculaire- critique de filtration de la membrane est convenablement de 5 000 - 100 000, compte tenu de la vitesse de filtration, de la séparation des protéines etc.The shape of the membrane is not particularly limited and it may for example be tubular, flat, spiral, in the form of hollow fibers, etc. The critical molecular filtering weight of the membrane is suitably 5,000-100,000, depending on the filtration rate, protein separation and so on.
Les exemples suivants illustrent l'invention sans toutefois en limiter la portée. The following examples illustrate the invention without, however, limiting its scope.
Exemple 1
On soumet à un traitement de chauffage å 60 s 70#C pendant 10 min 48 1 d'une liqueur d'acide glutamique obtenu par fermentation d'une liqueur d'amidon saccharifiée et ensuite on la soumet a l'ultrafiltration. Comme témoin, on soumet directement l'ultrafiltration 48 l de la même liqueur fermentée, dans les mimes conditions sans préchauffage.Example 1
A glutamic acid liquor obtained by fermentation of a saccharified starch liquor is subjected to a heating treatment at 60 ° C. for 10 minutes, and then subjected to ultrafiltration. As a control, the ultrafiltration 48 l of the same fermented liquor is directly subjected to the same conditions without preheating.
Les conditions de l'ultrafiltration sont les suivantes la membrane est une membrane de polysulfone ayant un poids moléculaire critique de filtration de 6 000 ; la structure de la membrane est sous forme de fibres creuses; la surface de la membrane est de 0,2 m2 ; la pression appliquée est de 100- 200 kPa et la température de filtration est de 400C. Dans l'un ou l'autre cas, on effectue la filtration jusqu'a ce que la quantité de filtrat soit de 38 1. Autrement dit, le degré de concentration dans l'un ou l'autre cas est de 4,8 fois. The conditions for ultrafiltration are as follows: the membrane is a polysulfone membrane having a critical molecular weight of 6,000 filtration; the structure of the membrane is in the form of hollow fibers; the surface of the membrane is 0.2 m2; the applied pressure is 100-200 kPa and the filtration temperature is 400C. In either case, the filtration is carried out until the amount of filtrate is 38 1. In other words, the degree of concentration in either case is 4.8 times. .
Les résultats obtenus sont indiqués dans le tableau ci-après. The results obtained are shown in the table below.
Comme on le voit clairement dans le tableau, dans le cas où l'on n'effectue pas de traitement de chauffage, on observe une réduction rapide du flux immédiatement après le début de la filtration, tandis que dans le cas où l'on effectue le traitement de chauffage (présente invention), la réduction du flux est extrtmement faible. I1 en résulte que la filtration est finie en 5 h dans le cas sans traitement thermique tandis que dans le cas avec traitement de chauffage elle est finie en 3 h, ctest-a-dire 60 % de la durée precédente. As can be clearly seen in the table, in the case where no heating treatment is carried out, a rapid reduction of the flow is observed immediately after the beginning of the filtration, whereas in the case where one carries out the heat treatment (present invention), the reduction of flux is extremely low. As a result, the filtration is finished in 5 h in the case without heat treatment while in the case with heating treatment it is finished in 3 h, that is to say 60% of the previous time.
Exemple 2
On soumet au chauffage à 90-1000C pendant 5 min 36 1 d'une liqueur de lysine obtenue par fermentation de mélasses de betterave et ensuite on la soumet à l'ultrafiltration. A titre de témoin, on soumet directement a l'ultrafiltration 36 1 de la mêmeliqueur de fermentation dans les mêmes conditions sans préchauffage.Example 2
A lysine liquor obtained by fermentation of beet molasses is subjected to heating at 90.degree.-100.degree. C. for 5 minutes and then subjected to ultrafiltration. As a control, 36 μl of the fermentation melter are subjected directly to the ultrafiltration under the same conditions without preheating.
Les conditions de l'ultrafiltration sont les suivantes la membrane est une membrane de polyacrylonitrile ayant un poids moléculaire critique de 13 000 ; la membrane a une structure en 2 fibres creuses et une surface de 0 2 m ; la pression appliquée est de 100 kPa et la température est de 400C. The conditions for ultrafiltration are as follows: the membrane is a polyacrylonitrile membrane having a critical molecular weight of 13,000; the membrane has a structure of 2 hollow fibers and a surface of 0 2 m; the applied pressure is 100 kPa and the temperature is 400C.
Dans l'un ou l'autre cas; la filtration est effectuée jusqu'à ce que le degré de concentration atteigne 10 fois,c'est à-dire que la quantité de la liqueur atteigre3,6 1. La durée de filtration nécessaire est de 3,8 h dans le cas sans chauffage ou 2,8 h dans le cas avec chauffage. Le flux moyen est de 43 1/mh, ou 58 l/mh, respectivement. In either case; the filtration is carried out until the degree of concentration reaches 10 times, that is to say that the amount of liquor reaches 3, 6 1. The necessary filtration time is 3.8 h in the case without heating or 2.8 h in the case with heating. The average flow is 43 1 / mh, or 58 l / mh, respectively.
Exemple 3
On soumet au chauffage à 950C pendant 3 min 20 1 d'une liqueur d'acide glutamique obtenue par fermentation de mélasses de canne à sucre puis on la soumet à l'ultrafiltration. Comme témoin, on soumet directement a l'ultrafiltration 20 1 de la même liqueur fermentée dans les mêmes conditions sans préchauffage.Example 3
A glutamic acid liquor obtained by fermentation of sugar cane molasses is subjected to heating at 950 ° C for 3 minutes and then subjected to ultrafiltration. As a control, 20 μl of the same fermented liquor are directly subjected to ultrafiltration under the same conditions without preheating.
Les conditions de l'ultrafiltration sont les suivantes la membrane est une membrane de polysulfone ayant une poids moléculaire critique de filtration de 6 000 et une structure en 2 filament creux ; la surface de la membrane est de 0,2 m , la pression appliquée est de 130 kPa et la température de filtration est de 600C. The conditions for ultrafiltration are as follows: the membrane is a polysulfone membrane having a critical filtration molecular weight of 6,000 and a hollow filament structure; the surface of the membrane is 0.2 m, the applied pressure is 130 kPa and the filtration temperature is 600C.
Dans l'un ou l'autre cas, on effectue la filtration jusqu'à ce que le degré de filtration atteigne 5 fois, c'est-à-dire que la quantité de la liqueur atteigne 10 1. La durée de filtration nécessaire est de 1,7 h sans chauffage ou 1,2 h avec chauffage. In either case, the filtration is carried out until the degree of filtration reaches 5 times, that is to say that the quantity of the liquor reaches 10 1. The required filtration time is 1.7 hours without heating or 1.2 hours with heating.
Le flux moyen est de 29 1/mh ou 42 1/mh, respectivement. The average flow is 29 1 / mh or 42 1 / mh, respectively.
On comprendra d'après ce qui précède que lorsque l'on soumet à l'ultrafiltration selon l'invention une liqueur de fer mentation microbienne, par exemple d'amino--acides, le leflux de ladite solution, c'est-à-dire la capacité de filtration de la membrane, est nettement amélioré et la solution peut etre traitée efficacement par ledit chauffage préalable de la solution selon l'invention. It will be understood from the above that when subjected to ultrafiltration according to the invention a microbial ironing liquor, for example amino acids, the flow of said solution, that is to say the filtration capacity of the membrane is significantly improved and the solution can be effectively treated by said preheating of the solution according to the invention.
il est entendu que l'invention ntest pas limitée aux modes de réalisation préférés décrits ci-dessus å titre d1illus- tration et que l'homme de l'art peut y apporter diverses modifications et divers changements sans toutefois s'écarter du cadre et de l'esprit de l'invention. It is to be understood that the invention is not limited to the above-described preferred embodiments of the invention and that those skilled in the art may make various modifications and changes thereto without departing from the scope and scope of the present invention. the spirit of the invention.
TABLEAU
<tb> Liqueur <SEP> traitée <SEP> par <SEP> chauffage <SEP> Liqueur <SEP> non <SEP> traitée <SEP> par <SEP> chauffage
<tb> <SEP> (présente <SEP> invention) <SEP> (témoin)
<tb> <SEP> Flux <SEP> Durée <SEP> de <SEP> filtration <SEP> Flux <SEP> Durée <SEP> de <SEP> filtration
<tb> (l/m2h) <SEP> (h,min) <SEP> (1/m2h) <SEP> <SEP> (h,min)
<tb> <SEP> 81 <SEP> 0,00 <SEP> <SEP> 81 <SEP> 0,00 <SEP>
<tb> <SEP> 74 <SEP> 0,23 <SEP> 57 <SEP> 0,15 <SEP>
<tb> <SEP> 72 <SEP> 0,52 <SEP> 46 <SEP> 0,45
<tb> <SEP> 66 <SEP> 1,15 <SEP> 42 <SEP> 1,15
<tb> <SEP> 61 <SEP> 2,00 <SEP> 38 <SEP> 2,00
<tb> <SEP> 56 <SEP> 2, <SEP> 30 <SEP> <SEP> 35 <SEP> 2,45 <SEP>
<tb> <SEP> 54 <SEP> 2,45 <SEP> 31 <SEP> 3,45
<tb> <SEP> 50 <SEP> 3100 <SEP> <SEP> 28 <SEP> 4,45
<tb> <SEP> 27 <SEP> 5,00
<tb> <tb> Liquor <SEP> treated <SEP> with <SEP> heating <SEP> Liquor <SEP> no <SEP> treated <SEP> with <SEP> heating
<tb><SEP> (present <SEP> invention) <SEP> (control)
<tb><SEP> Flow <SEP> Time <SEP> of <SEP> filtration <SEP> Flow <SEP> Time <SEP> of <SEP> filtration
<tb> (l / m2h) <SEP> (h, min) <SEP> (1 / m2h) <SEP><SEP> (h, min)
<tb><SEP> 81 <SEP> 0.00 <SEP><SEP> 81 <SEP> 0.00 <SEP>
<tb><SEP> 74 <SEP> 0.23 <SE> 57 <SEP> 0.15 <SEP>
<tb><SEP> 72 <SEP> 0.52 <SE> 46 <SEP> 0.45
<tb><SEP> 66 <SEP> 1.15 <SEP> 42 <SEP> 1.15
<tb><SEP> 61 <SEP> 2.00 <SEP> 38 <SEP> 2.00
<tb><SEP> 56 <SEP> 2, <SEP> 30 <SEP><SEP> 35 <SEP> 2.45 <SEP>
<tb><SEP> 54 <SEP> 2.45 <SEP> 31 <SEP> 3.45
<tb><SEP> 50 <SEP> 3100 <SEP><SEP> 28 <SEP> 4.45
<tb><SEP> 27 <SEP> 5.00
<Tb>
Claims (1)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58185312A JPS6078588A (en) | 1983-10-04 | 1983-10-04 | Improvement of flow flux of ultrafiltration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| FR2552674A1 true FR2552674A1 (en) | 1985-04-05 |
Family
ID=16168643
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8319862A Withdrawn FR2552674A1 (en) | 1983-10-04 | 1983-12-12 | Process for improving the flow in the ultrafiltration of the amino acids obtained by microbial fermentation. |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPS6078588A (en) |
| FR (1) | FR2552674A1 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2619380A1 (en) * | 1987-08-10 | 1989-02-17 | Ajinomoto Kk | PROCESS FOR THE RECOVERY OF L-AMINO ACIDS FROM FERMENTATION LIQUORS CONTAINING THEM |
| EP0714884A1 (en) * | 1994-11-30 | 1996-06-05 | Ajinomoto Co., Inc. | A method for the production of a basic amino acid |
| EP0768374A1 (en) * | 1995-10-13 | 1997-04-16 | Ajinomoto Co., Inc. | Method of removing cells from fermentation broth |
| EP0770676A3 (en) * | 1995-10-23 | 1999-05-19 | Ajinomoto Co., Ltd. | Method for treating fermentation broth |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3427223A (en) * | 1964-06-10 | 1969-02-11 | Exxon Research Engineering Co | Coagulating microbial cells to enhance their separation |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5791196A (en) * | 1980-11-27 | 1982-06-07 | Takeda Chem Ind Ltd | Separation of inosine, guanosine or their mixture from cell bodies and high polymeric substances |
| JPS5991196A (en) * | 1982-11-17 | 1984-05-25 | Dai Ichi Kogyo Seiyaku Co Ltd | Viscosity reducer for highly concentrated coke/water slurry |
-
1983
- 1983-10-04 JP JP58185312A patent/JPS6078588A/en active Granted
- 1983-12-12 FR FR8319862A patent/FR2552674A1/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3427223A (en) * | 1964-06-10 | 1969-02-11 | Exxon Research Engineering Co | Coagulating microbial cells to enhance their separation |
Non-Patent Citations (1)
| Title |
|---|
| W.F. BLATT et al.: "Membrane Science and Technology", 1970, pages 47-97, Plenum Press; "Cake Formation in Membrane Ultrafiltration" * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2619380A1 (en) * | 1987-08-10 | 1989-02-17 | Ajinomoto Kk | PROCESS FOR THE RECOVERY OF L-AMINO ACIDS FROM FERMENTATION LIQUORS CONTAINING THEM |
| US5017480A (en) * | 1987-08-10 | 1991-05-21 | Ajimomoto Co., Inc. | Process for recovering L-amino acid from fermentation liquors |
| EP0714884A1 (en) * | 1994-11-30 | 1996-06-05 | Ajinomoto Co., Inc. | A method for the production of a basic amino acid |
| EP0768374A1 (en) * | 1995-10-13 | 1997-04-16 | Ajinomoto Co., Inc. | Method of removing cells from fermentation broth |
| US5814513A (en) * | 1995-10-13 | 1998-09-29 | Ajinomoto Co., Inc. | Method of removing cells from fermentation broth through membrane |
| EP0770676A3 (en) * | 1995-10-23 | 1999-05-19 | Ajinomoto Co., Ltd. | Method for treating fermentation broth |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0529435B2 (en) | 1993-04-30 |
| JPS6078588A (en) | 1985-05-04 |
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