FR2401994A1 - Spreading microorganisms on cultivating gel in Petri dish - in continuous movement uninterrupted by sterilising spreader - Google Patents

Spreading microorganisms on cultivating gel in Petri dish - in continuous movement uninterrupted by sterilising spreader

Info

Publication number
FR2401994A1
FR2401994A1 FR7726789A FR7726789A FR2401994A1 FR 2401994 A1 FR2401994 A1 FR 2401994A1 FR 7726789 A FR7726789 A FR 7726789A FR 7726789 A FR7726789 A FR 7726789A FR 2401994 A1 FR2401994 A1 FR 2401994A1
Authority
FR
France
Prior art keywords
spreader
gel
prod
spreading
petri dish
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7726789A
Other languages
French (fr)
Other versions
FR2401994B3 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
API LABOR
Original Assignee
API LABOR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by API LABOR filed Critical API LABOR
Priority to FR7726789A priority Critical patent/FR2401994A1/en
Publication of FR2401994A1 publication Critical patent/FR2401994A1/en
Application granted granted Critical
Publication of FR2401994B3 publication Critical patent/FR2401994B3/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus

Abstract

Laboratory technique and appts. for spreading a prod. contg. microorganisms on the surface of a cultivation gel, partic. in a petri dish. The spreading is carried out without raising the spreader from the surface from start to finish of the operation. The spreader drains out the prod. in several lines of which at least adjacent pairs cross each other. At each intersection, the quantity of prod. drawn onwards by the spreader is progressively decreased. The spreader is pref. a flat plate of rigid material, e.g. plastic or metal, with a line of teeth on the underside to draw out the prod. from a single deposit on the surface of the gel. The teeth pref. lie on a straight line set at an angle between the direction of longitudinal displacement of the spreader and the perpendicular to that direction. The spreader is advanced longitudinally with simultaneous reciprocation at right angles to the direction of advancement, giving the teeth zig-zag tracks across the gel surface. Used for thinning out and sepg. a prod. contg. microorgansis on the surface of a nutrient gel in a petri dish. Spreading is carried out in a single, uninterrupted movement of a spreader which does not have to be removed, sterilised and cooled between spreading successive sets of intersecting line. This is quicker than previous techniques.
FR7726789A 1977-08-30 1977-08-30 Spreading microorganisms on cultivating gel in Petri dish - in continuous movement uninterrupted by sterilising spreader Granted FR2401994A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7726789A FR2401994A1 (en) 1977-08-30 1977-08-30 Spreading microorganisms on cultivating gel in Petri dish - in continuous movement uninterrupted by sterilising spreader

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7726789A FR2401994A1 (en) 1977-08-30 1977-08-30 Spreading microorganisms on cultivating gel in Petri dish - in continuous movement uninterrupted by sterilising spreader

Publications (2)

Publication Number Publication Date
FR2401994A1 true FR2401994A1 (en) 1979-03-30
FR2401994B3 FR2401994B3 (en) 1980-08-01

Family

ID=9195026

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7726789A Granted FR2401994A1 (en) 1977-08-30 1977-08-30 Spreading microorganisms on cultivating gel in Petri dish - in continuous movement uninterrupted by sterilising spreader

Country Status (1)

Country Link
FR (1) FR2401994A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR1000734B (en) * 1991-07-29 1992-11-23 Theodoros Anapliotis Description of a closed semiautomatic disposable petri dish system for the cultivation of microorganism
DE10011310A1 (en) * 2000-03-10 2001-09-27 Micro Med Ges Fuer Angewandte Dish for the cultivation of pathogens in a medical laboratory, comprises a seeding rake inserted into the lid opening with tines extending into the nutrient base and a closed lid forming a sealed system

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR1000734B (en) * 1991-07-29 1992-11-23 Theodoros Anapliotis Description of a closed semiautomatic disposable petri dish system for the cultivation of microorganism
DE10011310A1 (en) * 2000-03-10 2001-09-27 Micro Med Ges Fuer Angewandte Dish for the cultivation of pathogens in a medical laboratory, comprises a seeding rake inserted into the lid opening with tines extending into the nutrient base and a closed lid forming a sealed system
DE10011310C2 (en) * 2000-03-10 2002-02-28 Micro Med Ges Fuer Angewandte Device for growing seed cultures

Also Published As

Publication number Publication date
FR2401994B3 (en) 1980-08-01

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