FR2394085A1 - Fluorimetric analysis of physiologically active cpds. - such as alkaloid(s), medicaments, steroid(s), vitamin(s), sugars, prostaglandin(s), antibiotics and pesticides - Google Patents

Fluorimetric analysis of physiologically active cpds. - such as alkaloid(s), medicaments, steroid(s), vitamin(s), sugars, prostaglandin(s), antibiotics and pesticides

Info

Publication number
FR2394085A1
FR2394085A1 FR7717961A FR7717961A FR2394085A1 FR 2394085 A1 FR2394085 A1 FR 2394085A1 FR 7717961 A FR7717961 A FR 7717961A FR 7717961 A FR7717961 A FR 7717961A FR 2394085 A1 FR2394085 A1 FR 2394085A1
Authority
FR
France
Prior art keywords
coordinate
pesticides
antibiotics
sugars
medicaments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7717961A
Other languages
French (fr)
Other versions
FR2394085B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syva Co
Original Assignee
Syva Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syva Co filed Critical Syva Co
Priority to FR7717961A priority Critical patent/FR2394085A1/en
Publication of FR2394085A1 publication Critical patent/FR2394085A1/en
Application granted granted Critical
Publication of FR2394085B1 publication Critical patent/FR2394085B1/fr
Granted legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Inks, Pencil-Leads, Or Crayons (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The detection of a coordinate, i.e. any organic compound for which a natural receptor exists or can be prepared, or of an anti-coordinate, is effected as follows. A sample of the material under test is combined, in an aq. medium with (a) a coordinate analogue-fluorescent combination, the coordinate analogue being specifically recognised by the anticoordinate, and the coordinate and the fluorescent compound being attached sufficiently tightly by a bonding group that the simultaneous bonding of the anticoordinate and the fluorescent antibody undergoes tearic hindrance, (b) an anti-coordinate for the determination of the coordinate, and (c) an extinguisher combined with an antifluorescent agent, and the intensity of the fluorescence emitted by the medium at >=1 wavelength is compared with a control contg. a known quantity of the coordinate and anti-coordinate. Used in qualitative and quantitative determinations of alkaloids, synthetic medicaments, steroids, vitamins, sugars, prostaglandins, antibiotics, and pesticides. Interference from other materials present in the substance under test is minimised.
FR7717961A 1977-06-10 1977-06-10 Fluorimetric analysis of physiologically active cpds. - such as alkaloid(s), medicaments, steroid(s), vitamin(s), sugars, prostaglandin(s), antibiotics and pesticides Granted FR2394085A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7717961A FR2394085A1 (en) 1977-06-10 1977-06-10 Fluorimetric analysis of physiologically active cpds. - such as alkaloid(s), medicaments, steroid(s), vitamin(s), sugars, prostaglandin(s), antibiotics and pesticides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7717961A FR2394085A1 (en) 1977-06-10 1977-06-10 Fluorimetric analysis of physiologically active cpds. - such as alkaloid(s), medicaments, steroid(s), vitamin(s), sugars, prostaglandin(s), antibiotics and pesticides

Publications (2)

Publication Number Publication Date
FR2394085A1 true FR2394085A1 (en) 1979-01-05
FR2394085B1 FR2394085B1 (en) 1984-04-20

Family

ID=9191990

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7717961A Granted FR2394085A1 (en) 1977-06-10 1977-06-10 Fluorimetric analysis of physiologically active cpds. - such as alkaloid(s), medicaments, steroid(s), vitamin(s), sugars, prostaglandin(s), antibiotics and pesticides

Country Status (1)

Country Link
FR (1) FR2394085A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0017908A1 (en) * 1979-04-09 1980-10-29 Syva Company A method for determining the presence in a sample of an analyte and a kit for use in this method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2282115A1 (en) * 1974-08-12 1976-03-12 Syva Co METHOD FOR DETECTING IN A SOLUTION TO BE TITRATED, THE PRESENCE OF A COORDINATE IN AN UNKNOWN SUBSTANCE THAT MAY CONTAIN IT
US3998943A (en) * 1973-10-02 1976-12-21 Syva Company Double receptor fluorescent immunoassay
US4020151A (en) * 1976-02-17 1977-04-26 International Diagnostic Technology, Inc. Method for quantitation of antigens or antibodies on a solid surface

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3998943A (en) * 1973-10-02 1976-12-21 Syva Company Double receptor fluorescent immunoassay
FR2282115A1 (en) * 1974-08-12 1976-03-12 Syva Co METHOD FOR DETECTING IN A SOLUTION TO BE TITRATED, THE PRESENCE OF A COORDINATE IN AN UNKNOWN SUBSTANCE THAT MAY CONTAIN IT
US3996345A (en) * 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4020151A (en) * 1976-02-17 1977-04-26 International Diagnostic Technology, Inc. Method for quantitation of antigens or antibodies on a solid surface

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0017908A1 (en) * 1979-04-09 1980-10-29 Syva Company A method for determining the presence in a sample of an analyte and a kit for use in this method

Also Published As

Publication number Publication date
FR2394085B1 (en) 1984-04-20

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