FR2381764A1 - NEW DIPEPTIDIC DERIVATIVES OF GLYCYL-PROLYLAMINO-7-METHYL-4-COUMARINE AND METHOD FOR MEASURING ENZYMATIC ACTIVITY - Google Patents

NEW DIPEPTIDIC DERIVATIVES OF GLYCYL-PROLYLAMINO-7-METHYL-4-COUMARINE AND METHOD FOR MEASURING ENZYMATIC ACTIVITY

Info

Publication number
FR2381764A1
FR2381764A1 FR7805438A FR7805438A FR2381764A1 FR 2381764 A1 FR2381764 A1 FR 2381764A1 FR 7805438 A FR7805438 A FR 7805438A FR 7805438 A FR7805438 A FR 7805438A FR 2381764 A1 FR2381764 A1 FR 2381764A1
Authority
FR
France
Prior art keywords
methyl
prolylamino
glycyl
new
dipeptidic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR7805438A
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Publication of FR2381764A1 publication Critical patent/FR2381764A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06026—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atom, i.e. Gly or Ala
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/20—Coumarin derivatives
    • C12Q2337/22—7-Amino-4-methylcoumarin, i.e. AMC, MCA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)

Abstract

Pour mesurer l'activité de la X-prolyl dipeptidyl-aminopeptidase, on utilise un nouveau réactif constitué de glycyl-prolylamino-7 méthyl-4 coumarine ou un de ses sels d'acides que l'on met au contact de l'enzyme dans un milieu aqueux et on détermine l'amino-7 méthyl-4 coumarine formée par voie enzymatique. L'invention s'applique en particulier à des déterminations dans les liquides biologiques utiles pour le diagnostic des affections hépatiques et des cancers gastriques.To measure the activity of X-prolyl dipeptidyl-aminopeptidase, a new reagent is used consisting of 7-glycyl-prolylamino-4-methyl-coumarin or one of its acid salts which is brought into contact with the enzyme in an aqueous medium and the 7-amino-4-methyl-coumarin formed is determined enzymatically. The invention applies in particular to determinations in biological fluids useful for the diagnosis of liver diseases and gastric cancers.

FR7805438A 1977-02-26 1978-02-24 NEW DIPEPTIDIC DERIVATIVES OF GLYCYL-PROLYLAMINO-7-METHYL-4-COUMARINE AND METHOD FOR MEASURING ENZYMATIC ACTIVITY Withdrawn FR2381764A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2066377A JPS53105477A (en) 1977-02-26 1977-02-26 7-glycylprolylamono-4-methylcoumarine

Publications (1)

Publication Number Publication Date
FR2381764A1 true FR2381764A1 (en) 1978-09-22

Family

ID=12033434

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7805438A Withdrawn FR2381764A1 (en) 1977-02-26 1978-02-24 NEW DIPEPTIDIC DERIVATIVES OF GLYCYL-PROLYLAMINO-7-METHYL-4-COUMARINE AND METHOD FOR MEASURING ENZYMATIC ACTIVITY

Country Status (5)

Country Link
JP (1) JPS53105477A (en)
DE (1) DE2808111A1 (en)
FR (1) FR2381764A1 (en)
GB (1) GB1547747A (en)
SE (1) SE7801997L (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0018112A1 (en) * 1979-04-23 1980-10-29 Robert Eugene Smith Substrates and method for determining proteolytic enzymes

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5524147A (en) * 1978-08-10 1980-02-21 Ajinomoto Co Inc Peptide derivative
US5079144A (en) * 1982-04-14 1992-01-07 Radiometer Corporate Development Ltd. Microorganism testing with a hydrolyzable fluorogenic substrate
JPS59192099A (en) * 1983-04-12 1984-10-31 Ajinomoto Co Inc Measurement of number of microorganism
US5223401A (en) * 1988-11-29 1993-06-29 Minnesota Mining And Manufacturing Company Rapid read-out sterility indicator
US5073488A (en) * 1988-11-29 1991-12-17 Minnesota Mining And Manufacturing Company Rapid method for determining efficacy of a sterilization cycle and rapid read-out biological indicator
US5252484A (en) 1988-11-29 1993-10-12 Minnesota Mining And Manufacturing Company Rapid read-out biological indicator
DE19735614A1 (en) * 1997-08-16 1999-02-18 Lothar Dr Seik Detection of protease activity for insulin-like growth factor binding proteins
US8895239B2 (en) 2006-09-20 2014-11-25 American Sterilizer Company Genetically engineered biological indicator
US8043845B2 (en) 2006-09-20 2011-10-25 American Sterilizer Company Sterilization indicator
US8173388B2 (en) 2008-09-30 2012-05-08 American Sterilizer Company Self-contained biological indicator
EP2635699B1 (en) 2010-11-01 2018-03-21 3M Innovative Properties Company Biological sterilization indicator and method of using same
CN103189523B (en) 2010-11-01 2016-03-02 3M创新有限公司 Biological sterilization indicator
JP2014503196A (en) 2010-11-24 2014-02-13 スリーエム イノベイティブ プロパティズ カンパニー Methods and compositions for detecting biological activity
US20180237821A1 (en) 2017-02-23 2018-08-23 Ethicon, Inc. Apparatus and method to read biological indicator

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3259617A (en) * 1963-05-24 1966-07-05 Squibb & Sons Inc Process for purification of peptides
FR2329646A1 (en) * 1975-10-30 1977-05-27 Ajinomoto Kk NEW DIPEPTIDIC DERIVATIVES, CORRESPONDING SALTS AND METHOD FOR MEASURING AN ENZYMATIC ACTIVITY USING THEM

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3259617A (en) * 1963-05-24 1966-07-05 Squibb & Sons Inc Process for purification of peptides
FR2329646A1 (en) * 1975-10-30 1977-05-27 Ajinomoto Kk NEW DIPEPTIDIC DERIVATIVES, CORRESPONDING SALTS AND METHOD FOR MEASURING AN ENZYMATIC ACTIVITY USING THEM

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CA1976 *
CA1978 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0018112A1 (en) * 1979-04-23 1980-10-29 Robert Eugene Smith Substrates and method for determining proteolytic enzymes

Also Published As

Publication number Publication date
JPS6126558B2 (en) 1986-06-20
JPS53105477A (en) 1978-09-13
GB1547747A (en) 1979-06-27
SE7801997L (en) 1978-08-27
DE2808111A1 (en) 1978-08-31

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Legal Events

Date Code Title Description
RE Withdrawal of published application