ATE170929T1 - MEANS OF DETERMINING AN ANALYTE - Google Patents

MEANS OF DETERMINING AN ANALYTE

Info

Publication number
ATE170929T1
ATE170929T1 AT93108403T AT93108403T ATE170929T1 AT E170929 T1 ATE170929 T1 AT E170929T1 AT 93108403 T AT93108403 T AT 93108403T AT 93108403 T AT93108403 T AT 93108403T AT E170929 T1 ATE170929 T1 AT E170929T1
Authority
AT
Austria
Prior art keywords
analyte
determining
sample
indicator enzyme
hydrolase activity
Prior art date
Application number
AT93108403T
Other languages
German (de)
Inventor
Klaus Dr Pollmann
Helmut Dr Freitag
Anselm Dr Rothe
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Application granted granted Critical
Publication of ATE170929T1 publication Critical patent/ATE170929T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/12—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar
    • G01N2400/14—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar alpha-D-Glucans, i.e. having alpha 1,n (n=3,4,6) linkages between saccharide units, e.g. pullulan
    • G01N2400/22—Dextran
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/969—Multiple layering of reactants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a method for detecting a substance with hydrolase activity in a sample, characterised in that the sample is contacted with an indicator enzyme which is covalently bonded to an insoluble carrier material and which can be rendered soluble by the hydrolase activity, the indicator enzyme which has been cleaved off is separated off and its enzymatic activity is determined, and to a means for carrying it out.
AT93108403T 1992-05-27 1993-05-25 MEANS OF DETERMINING AN ANALYTE ATE170929T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4217474A DE4217474A1 (en) 1992-05-27 1992-05-27 Method and means for determining an analyte

Publications (1)

Publication Number Publication Date
ATE170929T1 true ATE170929T1 (en) 1998-09-15

Family

ID=6459797

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93108403T ATE170929T1 (en) 1992-05-27 1993-05-25 MEANS OF DETERMINING AN ANALYTE

Country Status (5)

Country Link
US (1) US5434054A (en)
EP (1) EP0571939B1 (en)
JP (1) JP2603422B2 (en)
AT (1) ATE170929T1 (en)
DE (2) DE4217474A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5416003A (en) * 1993-04-14 1995-05-16 Litmus Concepts, Inc. Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities
WO1994024306A1 (en) * 1993-04-14 1994-10-27 Litmus Concepts, Inc. Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities
AU4135096A (en) * 1994-11-07 1996-06-06 Litmus Concepts, Inc. Assay for proline iminopeptidase and other hydrolytic activities
US5571684A (en) * 1994-11-07 1996-11-05 Litmus Concepts, Inc. Assay for proline iminopeptidase and other hydrolytic activities
CA2269659A1 (en) * 1996-11-04 1998-05-14 Merck Frosst Canada & Co. Hydrolase binding assay
US7041469B2 (en) 2002-10-10 2006-05-09 Quidel Corporation Assays for trichomonal and other hydrolases
US8758989B2 (en) * 2006-04-06 2014-06-24 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
AU2007256364A1 (en) * 2006-06-09 2007-12-13 Bio Pur Ag A method for the detection of enzymatic reactions
US7935538B2 (en) * 2006-12-15 2011-05-03 Kimberly-Clark Worldwide, Inc. Indicator immobilization on assay devices
US20100290948A1 (en) * 2009-05-15 2010-11-18 Xuedong Song Absorbent articles capable of indicating the presence of urinary tract infections

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1320086A (en) * 1970-09-11 1973-06-13 Boehringer Mannheim Gmbh Agent and method for the determination of hydrolysing enzymes
US4098876A (en) * 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
CA1095819A (en) * 1977-01-14 1981-02-17 Eastman Kodak Company Element for analysis of liquids
US4331590A (en) * 1978-03-13 1982-05-25 Miles Laboratories, Inc. β-Galactosyl-umbelliferone-labeled protein and polypeptide conjugates
GB2059421A (en) * 1979-10-03 1981-04-23 Self C H Assay method and reagents therefor
DE3029579C2 (en) * 1980-08-05 1985-12-12 Boehringer Mannheim Gmbh, 6800 Mannheim Method and means for separating plasma or serum from whole blood
US4590157A (en) * 1980-12-22 1986-05-20 Commonwealth Serum Laboratories Commission Method for detecting antigens and antibodies
EP0060123B1 (en) * 1981-03-07 1986-10-29 Colin Henry Self Assay and use
CA1209889A (en) * 1981-09-18 1986-08-19 Litai Weng Homogeneous assay on a non-porous surface
US4446232A (en) * 1981-10-13 1984-05-01 Liotta Lance A Enzyme immunoassay with two-zoned device having bound antigens
DE3247608A1 (en) * 1982-12-23 1984-07-05 Boehringer Mannheim Gmbh, 6800 Mannheim TEST STRIP
DK147184A (en) * 1983-04-18 1984-10-19 Du Pont Device for removing pelt mounting poles
DE3485339D1 (en) * 1983-11-18 1992-01-23 Fujirebio Kk METHOD FOR MEASURING A BIOLOGICAL LIGAND.
DE3345748A1 (en) * 1983-12-17 1985-08-29 Boehringer Mannheim Gmbh, 6800 Mannheim PHENOLSULPHONPHTHALEINYL-SS-D-GALACTOSIDE, METHOD FOR THE PRODUCTION THEREOF AND THE USE THEREOF FOR DETERMINING THE SS-D-GALACTOSIDASE
US4828982A (en) * 1985-05-28 1989-05-09 Becton, Dickinson & Company Vesticles and use thereof in an enzyme assay
US4874710A (en) * 1986-02-20 1989-10-17 Becton Dickinson And Company Assay and product in which binder and liposomes are supported on a solid support
US4859581A (en) * 1986-03-10 1989-08-22 Board Of Regents, The University Of Texas System Endoglycosidase assay
JPS62265998A (en) * 1986-03-10 1987-11-18 ボ−ド　オブ　リ−ジエンツ　ザ　ユニヴア−シテイ　オブ　テキサス　システム Endoglycosidase assay
US5190864A (en) * 1986-04-15 1993-03-02 Northeastern University Enzyme amplification by using free enzyme to release enzyme from an immobilized enzyme material
DE3638654A1 (en) * 1986-11-12 1988-05-26 Boehringer Mannheim Gmbh TEST CARRIER FOR ANALYTICAL DETERMINATION OF A COMPONENT OF A BODY LIQUID
DE3715485A1 (en) * 1987-05-09 1988-11-17 Boehringer Mannheim Gmbh STABILIZATION OF SS GALACTOSIDASE
US4952495A (en) * 1987-06-08 1990-08-28 Eastman Kodak Company Hydrolyzable compounds which release electron transfer agents and analytical use of same
EP0347256A3 (en) * 1988-06-17 1990-07-11 Tosoh Corporation Method for detecting a substance in a sample
DE68918504T2 (en) * 1988-06-24 1995-03-09 Fuji Photo Film Co Ltd Dry type analysis element for an immunoassay.
WO1990000252A1 (en) * 1988-06-27 1990-01-11 Beckman Instruments, Inc. Method for specific binding assays
US4962024A (en) * 1988-08-11 1990-10-09 Becton, Dickinson And Company Signal enhancement in assay for an enzyme
US5071745A (en) * 1988-12-14 1991-12-10 Elcatech, Inc. Fibrinolytic assay
US5215885A (en) * 1990-10-23 1993-06-01 Beckman Instruments, Inc. Stable two-part chromogen substrate
US5235039A (en) * 1991-06-10 1993-08-10 Eli Lilly And Company Substrates for hiv protease

Also Published As

Publication number Publication date
DE4217474A1 (en) 1993-12-02
JP2603422B2 (en) 1997-04-23
JPH0646893A (en) 1994-02-22
EP0571939B1 (en) 1998-09-09
DE59308966D1 (en) 1998-10-15
US5434054A (en) 1995-07-18
EP0571939A1 (en) 1993-12-01

Similar Documents

Publication Publication Date Title
YU11500A (en) Method and apparatus for rapid analysis in biological samples
DE3870537D1 (en) IMMUNDIAGNOSTIC DEVICE AND RELATED METHOD.
CA2332112A1 (en) Monitoring of physiological analytes
DE69914319D1 (en) SIGNAL PROCESSING FOR MEASURING PHYSIOLOGICAL ANALYTES
EP0753148A1 (en) Saliva assay method and device
ES2077061T3 (en) METHOD AND REAGENT FOR THE DETERMINATION OF AN ANALYSIS MATERIAL THROUGH ENZYMATIC MEDIA USING A FERRICIANIDE / FERRIC COMPOSITE SYSTEM.
DE69028247D1 (en) Method for measuring constituents of a body fluid
ATE170929T1 (en) MEANS OF DETERMINING AN ANALYTE
ATE131527T1 (en) MEANS AND METHODS OF ANALYSIS
ES8507265A1 (en) Method of measuring biological ligands.
ATE73500T1 (en) ANALYTICAL METHODS AND MEANS FOR DETECTING ESTEROLYTIC AND/OR PROTEOLYTIC ENZYMES.
DE3850249D1 (en) DETECTION OF ANALYTES IN SAMPLE CONTAINING PARTICLES.
JPS5794660A (en) Measurement of inhibitor-enzyme compound
ATE74211T1 (en) PROCEDURE FOR DETECTING AN ANALYTE AND MEANS THEREOF.
Yao et al. On‐line amperometric assay of glucose, l‐glutamate, and acetylcholine using microdialysis probes and immobilized enzyme reactors
MX9604417A (en) Detection of analytes.
DE68922550D1 (en) Alcohol oxidase enzyme electrode and method for determining alcohol content.
EP0176246A3 (en) Method and kit for detecting the presence of gum disease
AU655405B2 (en) Enzymatic method for determining analyte concentrations
DE69229954D1 (en) NUCLEIC ACID DETECTION
DE59108478D1 (en) Functional test to determine protein S activity
ATE269417T1 (en) METHOD FOR DETECTING AND MEASURING SPLICED NUCLEIC ACIDS
IL135394A0 (en) Diffusion-controlling sensor layer
DE59509639D1 (en) METHOD AND ELECTROCHEMICAL-ENZYMATIC INDICATION SYSTEM FOR DETERMINING NITRITE
ATE107031T1 (en) PROCEDURE FOR DETERMINING A LIGAND.

Legal Events

Date Code Title Description
REN Ceased due to non-payment of the annual fee