FR2367116A1 - PROCESS FOR THE PREPARATION OF OLIGOSACCHARIDES - Google Patents

PROCESS FOR THE PREPARATION OF OLIGOSACCHARIDES

Info

Publication number
FR2367116A1
FR2367116A1 FR7730035A FR7730035A FR2367116A1 FR 2367116 A1 FR2367116 A1 FR 2367116A1 FR 7730035 A FR7730035 A FR 7730035A FR 7730035 A FR7730035 A FR 7730035A FR 2367116 A1 FR2367116 A1 FR 2367116A1
Authority
FR
France
Prior art keywords
oligosaccharides
preparation
sample
olisosaccharides
amylases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7730035A
Other languages
French (fr)
Other versions
FR2367116B1 (en
Inventor
Leo M Hall
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Alabama UA
University of Alabama at Birmingham UAB
Original Assignee
University of Alabama UA
University of Alabama at Birmingham UAB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05/730,820 external-priority patent/US4081326A/en
Priority claimed from US05/742,240 external-priority patent/US4071407A/en
Application filed by University of Alabama UA, University of Alabama at Birmingham UAB filed Critical University of Alabama UA
Publication of FR2367116A1 publication Critical patent/FR2367116A1/en
Application granted granted Critical
Publication of FR2367116B1 publication Critical patent/FR2367116B1/fr
Granted legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • C12N1/185Saccharomyces isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/85Saccharomyces
    • C12R2001/865Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention est relative à un procédé pour la préparation d'oligosaccharides. Ce procédé est caractérisé en ce que l'on élimine pratiquement tous les olisosaccharides en dessous d'un poids moléculaire minimum désiré, à partir d'un échantillon contenant un mélange d'oligosaccharides, en traitant simultanément ledit échantillon par une levure et une amylase. Application d'oligosaccharides ainsi obtenus comme substrats pour le dosage des amylases.The present invention relates to a process for the preparation of oligosaccharides. This process is characterized in that practically all olisosaccharides below a desired minimum molecular weight are removed from a sample containing a mixture of oligosaccharides, by simultaneously treating said sample with yeast and amylase. Application of oligosaccharides thus obtained as substrates for the assay of amylases.

FR7730035A 1976-10-07 1977-10-06 PROCESS FOR THE PREPARATION OF OLIGOSACCHARIDES Granted FR2367116A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US05/730,820 US4081326A (en) 1976-10-07 1976-10-07 A method for removing maltotetraose and lower saccharides from solution
US05/742,240 US4071407A (en) 1976-11-16 1976-11-16 Novel maltase enzyme produced by a new yeast strain

Publications (2)

Publication Number Publication Date
FR2367116A1 true FR2367116A1 (en) 1978-05-05
FR2367116B1 FR2367116B1 (en) 1983-07-18

Family

ID=27112116

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7730035A Granted FR2367116A1 (en) 1976-10-07 1977-10-06 PROCESS FOR THE PREPARATION OF OLIGOSACCHARIDES

Country Status (7)

Country Link
JP (1) JPS5391150A (en)
AU (1) AU514968B2 (en)
BE (2) BE859404A (en)
DE (1) DE2745206A1 (en)
FR (1) FR2367116A1 (en)
GB (1) GB1558538A (en)
NL (2) NL7711008A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3788910A (en) * 1972-01-17 1974-01-29 Labatt Breweries Canada Ltd Extraction and purification of maltotriose and maltotetrose
FR2243437A1 (en) * 1973-09-06 1975-04-04 Du Pont

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3788910A (en) * 1972-01-17 1974-01-29 Labatt Breweries Canada Ltd Extraction and purification of maltotriose and maltotetrose
FR2243437A1 (en) * 1973-09-06 1975-04-04 Du Pont

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CA1969 *
CA1973 *
EXBK/55 *

Also Published As

Publication number Publication date
BE859404A (en) 1978-04-05
BE859405A (en) 1978-04-05
GB1558538A (en) 1980-01-03
JPS5612437B2 (en) 1981-03-20
NL7711009A (en) 1978-04-11
DE2745206A1 (en) 1978-04-13
AU2945377A (en) 1979-04-12
JPS5391150A (en) 1978-08-10
FR2367116B1 (en) 1983-07-18
DE2745206C2 (en) 1987-07-23
NL7711008A (en) 1978-04-11
AU514968B2 (en) 1981-03-12

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