FR2366306A1 - Nouveau procede de preparation de clostripaine pure et clostripaine pure lyophilisee obtenue par ce procede - Google Patents
Nouveau procede de preparation de clostripaine pure et clostripaine pure lyophilisee obtenue par ce procedeInfo
- Publication number
- FR2366306A1 FR2366306A1 FR7604807A FR7604807A FR2366306A1 FR 2366306 A1 FR2366306 A1 FR 2366306A1 FR 7604807 A FR7604807 A FR 7604807A FR 7604807 A FR7604807 A FR 7604807A FR 2366306 A1 FR2366306 A1 FR 2366306A1
- Authority
- FR
- France
- Prior art keywords
- clostripain
- arginine
- pure
- buffer
- affinity chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090001092 clostripain Proteins 0.000 title abstract 4
- 102000029816 Collagenase Human genes 0.000 title abstract 2
- 108060005980 Collagenase Proteins 0.000 title abstract 2
- 241000193159 Hathewaya histolytica Species 0.000 title abstract 2
- 238000001042 affinity chromatography Methods 0.000 title abstract 2
- 229960002424 collagenase Drugs 0.000 title abstract 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 abstract 2
- WPANETAWYGDRLL-UHFFFAOYSA-N 4-aminobenzenecarboximidamide Chemical compound NC(=N)C1=CC=C(N)C=C1 WPANETAWYGDRLL-UHFFFAOYSA-N 0.000 abstract 1
- 239000004475 Arginine Substances 0.000 abstract 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 abstract 1
- 229930064664 L-arginine Natural products 0.000 abstract 1
- 235000014852 L-arginine Nutrition 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 1
- 235000009697 arginine Nutrition 0.000 abstract 1
- 238000004108 freeze drying Methods 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 229920000724 poly(L-arginine) polymer Polymers 0.000 abstract 1
- 150000003141 primary amines Chemical group 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7604807A FR2366306A1 (fr) | 1976-02-20 | 1976-02-20 | Nouveau procede de preparation de clostripaine pure et clostripaine pure lyophilisee obtenue par ce procede |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7604807A FR2366306A1 (fr) | 1976-02-20 | 1976-02-20 | Nouveau procede de preparation de clostripaine pure et clostripaine pure lyophilisee obtenue par ce procede |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2366306A1 true FR2366306A1 (fr) | 1978-04-28 |
| FR2366306B1 FR2366306B1 (enExample) | 1979-05-04 |
Family
ID=9169415
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR7604807A Granted FR2366306A1 (fr) | 1976-02-20 | 1976-02-20 | Nouveau procede de preparation de clostripaine pure et clostripaine pure lyophilisee obtenue par ce procede |
Country Status (1)
| Country | Link |
|---|---|
| FR (1) | FR2366306A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0518088A3 (enExample) * | 1991-05-23 | 1994-03-23 | Hoechst Ag |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2137610A1 (enExample) * | 1971-05-12 | 1972-12-29 | Xerox Corp |
-
1976
- 1976-02-20 FR FR7604807A patent/FR2366306A1/fr active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2137610A1 (enExample) * | 1971-05-12 | 1972-12-29 | Xerox Corp |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0518088A3 (enExample) * | 1991-05-23 | 1994-03-23 | Hoechst Ag |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2366306B1 (enExample) | 1979-05-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |