FR2307817A1 - Insolubilised glucose isomerase adsorbed on anion exchange resin - giving increased activity and longer life in converting glucose to fructose - Google Patents

Insolubilised glucose isomerase adsorbed on anion exchange resin - giving increased activity and longer life in converting glucose to fructose

Info

Publication number
FR2307817A1
FR2307817A1 FR7511541A FR7511541A FR2307817A1 FR 2307817 A1 FR2307817 A1 FR 2307817A1 FR 7511541 A FR7511541 A FR 7511541A FR 7511541 A FR7511541 A FR 7511541A FR 2307817 A1 FR2307817 A1 FR 2307817A1
Authority
FR
France
Prior art keywords
glucose
resin
fructose
insolubilised
glucose isomerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7511541A
Other languages
French (fr)
Other versions
FR2307817B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to FR7511541A priority Critical patent/FR2307817A1/en
Publication of FR2307817A1 publication Critical patent/FR2307817A1/en
Application granted granted Critical
Publication of FR2307817B1 publication Critical patent/FR2307817B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds

Abstract

An insolubilised glucose isomerase (I) is prepd. by adsorbing and bonding glucose isomerase onto an anion exchange resin with porosity >4.5% measured by the aq. dextran soln. method and with ion exchange capacity 0.035 meq/ml resin measured by the poly-anion salt analysis method. Isomerisation of glucose to fructose by means of (I) is claimed. The prod. has increased and prolonged enzymatic activity, and is more resistant to crumbling and deformation. There is less press. loss in the column through which the glucose soln. passes. The yield/unit of time is greater. The fructose prod. is not discoloured. The resin esp. has porosity 7-50% (7-20%) and ion exchange capacity 0.035-0.3 (>0.05) pref. 0.05-0.1 meq/ml resin. The resin is pref. a styrene-divinylbenzene copolymer carrying quat. ammonium gps., in partic. trimethylammonium or dimethylethanolammonium gps. The glucose isomerase is derived from Actinomycetes.
FR7511541A 1975-04-14 1975-04-14 Insolubilised glucose isomerase adsorbed on anion exchange resin - giving increased activity and longer life in converting glucose to fructose Granted FR2307817A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7511541A FR2307817A1 (en) 1975-04-14 1975-04-14 Insolubilised glucose isomerase adsorbed on anion exchange resin - giving increased activity and longer life in converting glucose to fructose

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7511541A FR2307817A1 (en) 1975-04-14 1975-04-14 Insolubilised glucose isomerase adsorbed on anion exchange resin - giving increased activity and longer life in converting glucose to fructose

Publications (2)

Publication Number Publication Date
FR2307817A1 true FR2307817A1 (en) 1976-11-12
FR2307817B1 FR2307817B1 (en) 1977-11-25

Family

ID=9153889

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7511541A Granted FR2307817A1 (en) 1975-04-14 1975-04-14 Insolubilised glucose isomerase adsorbed on anion exchange resin - giving increased activity and longer life in converting glucose to fructose

Country Status (1)

Country Link
FR (1) FR2307817A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0097898A2 (en) * 1982-06-26 1984-01-11 BASF Aktiengesellschaft Macroporous hydrophilic enzyme carrier

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2243954A1 (en) * 1973-09-13 1975-04-11 Cpc International Inc

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2243954A1 (en) * 1973-09-13 1975-04-11 Cpc International Inc

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0097898A2 (en) * 1982-06-26 1984-01-11 BASF Aktiengesellschaft Macroporous hydrophilic enzyme carrier
EP0097898A3 (en) * 1982-06-26 1986-01-15 Basf Aktiengesellschaft Macroporous hydrophilic enzyme carrier

Also Published As

Publication number Publication date
FR2307817B1 (en) 1977-11-25

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