FR2275482A1 - Fixing polypeptides to macromolecular support - by generating polypeptide radicals in aq. soln. contg. the support - Google Patents

Fixing polypeptides to macromolecular support - by generating polypeptide radicals in aq. soln. contg. the support

Info

Publication number
FR2275482A1
FR2275482A1 FR7518876A FR7518876A FR2275482A1 FR 2275482 A1 FR2275482 A1 FR 2275482A1 FR 7518876 A FR7518876 A FR 7518876A FR 7518876 A FR7518876 A FR 7518876A FR 2275482 A1 FR2275482 A1 FR 2275482A1
Authority
FR
France
Prior art keywords
support
contg
soln
fixing
polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7518876A
Other languages
French (fr)
Other versions
FR2275482B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roehm GmbH Darmstadt
Original Assignee
Roehm GmbH Darmstadt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roehm GmbH Darmstadt filed Critical Roehm GmbH Darmstadt
Publication of FR2275482A1 publication Critical patent/FR2275482A1/en
Application granted granted Critical
Publication of FR2275482B1 publication Critical patent/FR2275482B1/fr
Granted legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/44—Antibodies bound to carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/098—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer formed in the presence of the enzymes or microbial cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers

Abstract

The prepn. of polypeptides fixed to a support comprises generating radicals in an aq. soln. of the polypeptide (I) in presence of a macromolecular cpd. (II) contg. at least one olefinic double bond per molecule, and in the absence of a radical-polymerisable monomer. Esp. useful for prepn. of immobilised enzymes, enzyme inhibitors, antibodies, or hormones, used in various biochemical processes, medicine diagnostics, etc. The process is also applicable to cpds. having a partial peptide structure, e.g. penicillins, or for reacting non-peptides with oligopeptides and fixing them to macromolecules via these gps.
FR7518876A 1974-06-22 1975-06-17 Fixing polypeptides to macromolecular support - by generating polypeptide radicals in aq. soln. contg. the support Granted FR2275482A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2430128A DE2430128C3 (en) 1974-06-22 1974-06-22 Process for the preparation of carrier-bound polypeptides

Publications (2)

Publication Number Publication Date
FR2275482A1 true FR2275482A1 (en) 1976-01-16
FR2275482B1 FR2275482B1 (en) 1978-10-13

Family

ID=5918762

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7518876A Granted FR2275482A1 (en) 1974-06-22 1975-06-17 Fixing polypeptides to macromolecular support - by generating polypeptide radicals in aq. soln. contg. the support

Country Status (4)

Country Link
BE (1) BE830479A (en)
CH (1) CH596234A5 (en)
DE (1) DE2430128C3 (en)
FR (1) FR2275482A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0081408A1 (en) * 1981-11-19 1983-06-15 Société d'Expansion Scientifique Expansia Ethylene diamine functional acrylic resins and preparation thereof
EP0251121A2 (en) * 1986-06-28 1988-01-07 Röhm Gmbh Process for inducing the formation of antibodies

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE361320B (en) * 1972-03-14 1973-10-29 Exploaterings Ab Tbf

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0081408A1 (en) * 1981-11-19 1983-06-15 Société d'Expansion Scientifique Expansia Ethylene diamine functional acrylic resins and preparation thereof
EP0251121A2 (en) * 1986-06-28 1988-01-07 Röhm Gmbh Process for inducing the formation of antibodies
EP0251121A3 (en) * 1986-06-28 1989-12-06 Röhm Gmbh Process for inducing the formation of antibodies

Also Published As

Publication number Publication date
FR2275482B1 (en) 1978-10-13
DE2430128B2 (en) 1980-08-07
CH596234A5 (en) 1978-03-15
DE2430128A1 (en) 1976-01-15
DE2430128C3 (en) 1981-11-05
BE830479A (en) 1975-10-16

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