FR2101223A1 - Bacteria determination in urine - by bioluminescence reaction of bacterial atp - Google Patents

Bacteria determination in urine - by bioluminescence reaction of bacterial atp

Info

Publication number
FR2101223A1
FR2101223A1 FR7129294A FR7129294A FR2101223A1 FR 2101223 A1 FR2101223 A1 FR 2101223A1 FR 7129294 A FR7129294 A FR 7129294A FR 7129294 A FR7129294 A FR 7129294A FR 2101223 A1 FR2101223 A1 FR 2101223A1
Authority
FR
France
Prior art keywords
atp
bacterial
urine
bioluminescence reaction
bacterial atp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7129294A
Other languages
French (fr)
Other versions
FR2101223B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Aeronautics and Space Administration NASA
Original Assignee
National Aeronautics and Space Administration NASA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Aeronautics and Space Administration NASA filed Critical National Aeronautics and Space Administration NASA
Publication of FR2101223A1 publication Critical patent/FR2101223A1/en
Application granted granted Critical
Publication of FR2101223B1 publication Critical patent/FR2101223B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Bacteria in urine samples are detected and counted by a highly sensitive, rapid, accurate and reproducible procedure in which (a) non-bacterial ATP-contg. cells are disintegrated (e.g. by a pref. anionic or nonionic detergent), (b) the non-bacterial ATP is decomposed (e.g. by an ATPase), (c) the ATP-decomposing agent is removed, (d) the bacterial cells are disintegrated to liberate their ATP, (e) the pH is adjusted to a value (pref. 7.4) favourable for a bioluminescence reaction of ATP with a luciferase-luciferine mixture, (f) a luciferase-luciferine mixture contg. Mg++ or Mn++ ions is added, and (g) the quantity of light emitted is determined.
FR7129294A 1970-08-04 1971-08-04 Bacteria determination in urine - by bioluminescence reaction of bacterial atp Granted FR2101223A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US6095070A 1970-08-04 1970-08-04

Publications (2)

Publication Number Publication Date
FR2101223A1 true FR2101223A1 (en) 1972-03-31
FR2101223B1 FR2101223B1 (en) 1973-06-29

Family

ID=22032754

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7129294A Granted FR2101223A1 (en) 1970-08-04 1971-08-04 Bacteria determination in urine - by bioluminescence reaction of bacterial atp

Country Status (1)

Country Link
FR (1) FR2101223A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1539147A1 (en) * 2002-07-09 2005-06-15 Fasgen, LLC Methods of treating microbial infections in humans and animals

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1539147A1 (en) * 2002-07-09 2005-06-15 Fasgen, LLC Methods of treating microbial infections in humans and animals
EP1539147A4 (en) * 2002-07-09 2007-04-25 Fasgen Llc Methods of treating microbial infections in humans and animals

Also Published As

Publication number Publication date
FR2101223B1 (en) 1973-06-29

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Legal Events

Date Code Title Description
ST Notification of lapse