FI953141A0 - Method for Isolation and Purification of HIV Glycoprotein GP 160, Produced in Cells - Google Patents

Method for Isolation and Purification of HIV Glycoprotein GP 160, Produced in Cells

Info

Publication number
FI953141A0
FI953141A0 FI953141A FI953141A FI953141A0 FI 953141 A0 FI953141 A0 FI 953141A0 FI 953141 A FI953141 A FI 953141A FI 953141 A FI953141 A FI 953141A FI 953141 A0 FI953141 A0 FI 953141A0
Authority
FI
Finland
Prior art keywords
cells
isolation
purification
produced
hiv glycoprotein
Prior art date
Application number
FI953141A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI97058B (en
FI97058C (en
FI953141A (en
Inventor
Johann Eibl
Friedrich Dorner
Original Assignee
Immuno Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immuno Ag filed Critical Immuno Ag
Publication of FI953141A0 publication Critical patent/FI953141A0/en
Publication of FI953141A publication Critical patent/FI953141A/en
Publication of FI97058B publication Critical patent/FI97058B/en
Application granted granted Critical
Publication of FI97058C publication Critical patent/FI97058C/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Engineering & Computer Science (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

Proteins produced in cells collect together to form particulate aggregates. The invention relates to the reversible conversion of said aggregated proteins into morphological forms I, II and III which differ from one another, depending on the presence or absence of a non-denaturing detergent. The invention also relates to a process for the isolation and purification of said aggregated forms of proteins produced in cells.
FI953141A 1987-12-23 1995-06-22 Method for isolating and purifying HIV glycoprotein GP 160 produced in cells FI97058C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP87119172 1987-12-23
EP87119172A EP0321606B1 (en) 1987-12-23 1987-12-23 Cellular amphipatic proteins in aggregated forms, and procedure for the production and purification of these proteins

Publications (4)

Publication Number Publication Date
FI953141A0 true FI953141A0 (en) 1995-06-22
FI953141A FI953141A (en) 1995-06-22
FI97058B FI97058B (en) 1996-06-28
FI97058C FI97058C (en) 1996-10-10

Family

ID=8197541

Family Applications (2)

Application Number Title Priority Date Filing Date
FI885629A FI96864C (en) 1987-12-23 1988-12-02 Non-therapeutically useful HIV glycoprotein GP 160 and method for its preparation
FI953141A FI97058C (en) 1987-12-23 1995-06-22 Method for isolating and purifying HIV glycoprotein GP 160 produced in cells

Family Applications Before (1)

Application Number Title Priority Date Filing Date
FI885629A FI96864C (en) 1987-12-23 1988-12-02 Non-therapeutically useful HIV glycoprotein GP 160 and method for its preparation

Country Status (8)

Country Link
EP (1) EP0321606B1 (en)
JP (1) JP2656098B2 (en)
AT (1) ATE95188T1 (en)
DE (1) DE3787654D1 (en)
DK (1) DK628188A (en)
ES (1) ES2059355T3 (en)
FI (2) FI96864C (en)
NO (1) NO175782C (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2672895B1 (en) * 1991-02-15 1995-05-12 Transgene Sa PROCESS FOR THE PURIFICATION OF A HIGHLY GLYCOSYLATED PROTEIN.
FR2692898B1 (en) * 1992-06-30 1995-06-02 Centre Nat Rech Scient Method for obtaining membrane proteins, and use of these proteins for diagnostic or vaccination purposes.
US5914390A (en) * 1997-05-12 1999-06-22 Celtrix Pharmaceuticals, Inc. Methods for increasing yields of recombinant proteins
DE19939246A1 (en) 1999-08-19 2001-02-22 Phasys Gmbh M Refolding membrane proteins into their native or active form comprises solubilizing the protein in a first detergent and replacing the first detergent with a second detergent

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61233700A (en) * 1984-12-24 1986-10-17 ジエネンテク，インコ−ポレイテツド Molecularly cloned aids related polypeptide
CN86100979A (en) * 1985-02-07 1986-12-17 史密丝克莱恩贝克曼公司 The preparation method of malaria vaccine
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
JPS62198627A (en) * 1986-02-26 1987-09-02 Biseibutsu Kagaku Kenkyusho:Kk Aujesky's disease solubilizing antigen vaccine

Also Published As

Publication number Publication date
DE3787654D1 (en) 1993-11-04
FI96864B (en) 1996-05-31
FI97058B (en) 1996-06-28
NO885738D0 (en) 1988-12-23
EP0321606B1 (en) 1993-09-29
NO175782C (en) 1994-12-07
ES2059355T3 (en) 1994-11-16
ATE95188T1 (en) 1993-10-15
JPH02798A (en) 1990-01-05
FI96864C (en) 1996-09-10
DK628188A (en) 1989-06-24
FI97058C (en) 1996-10-10
NO885738L (en) 1989-06-26
FI953141A (en) 1995-06-22
NO175782B (en) 1994-08-29
FI885629A0 (en) 1988-12-02
DK628188D0 (en) 1988-11-10
FI885629A (en) 1989-06-24
EP0321606A1 (en) 1989-06-28
JP2656098B2 (en) 1997-09-24

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