FI945266A - Mannanase enzyme and corresp. DNA - are produced in recombinant yeast or fungi; used for hydrolysis of manno-polymers, esp. in bleaching lignocellulose pulps - Google Patents

Mannanase enzyme and corresp. DNA - are produced in recombinant yeast or fungi; used for hydrolysis of manno-polymers, esp. in bleaching lignocellulose pulps Download PDF

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Publication number
FI945266A
FI945266A FI945266A FI945266A FI945266A FI 945266 A FI945266 A FI 945266A FI 945266 A FI945266 A FI 945266A FI 945266 A FI945266 A FI 945266A FI 945266 A FI945266 A FI 945266A
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FI
Finland
Prior art keywords
pref
enzyme
hydrolysis
dna
contg
Prior art date
Application number
FI945266A
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Finnish (fi)
Swedish (sv)
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FI118264B (en
FI945266A0 (en
Inventor
Johanna Buchert
Matti Siika-Aho
Liisa Viikari
Merja Penttilae
Anu Saloheimo
Marjatta Ranua
Original Assignee
Alko Ab Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FI922373A external-priority patent/FI922373A0/en
Priority claimed from FI931193A external-priority patent/FI931193A0/en
Application filed by Alko Ab Oy filed Critical Alko Ab Oy
Priority to FI945266A priority Critical patent/FI118264B/en
Publication of FI945266A0 publication Critical patent/FI945266A0/en
Publication of FI945266A publication Critical patent/FI945266A/en
Application granted granted Critical
Publication of FI118264B publication Critical patent/FI118264B/en

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Paper (AREA)

Abstract

DNA (I) encoding a Trichoderma enzyme having mannanase (MN) activity is new, which when transferred into a yeast or fungus strain induces that strain to produce MN. Also new are (a) a vector contg. (I), (b) a recombinant yeast or fungus strain contg. (I), (c) a process for producing a MN or enzyme mixt. by (i) treating a T. reesei culture medium with bentonite, (ii) subjecting the medium to first cation exchange then to anion exchange at pH 7-7.5, (iii) collecting the eluates and purifying by hydrophobic interaction chromatography, and (iv) opt. subjecting the prod. to additional purificn., and (d) an enzyme prepn. contg. at least 1 MN produced by Trichoderma with MN activity and an isoelectric point (pI) of about 3.8, 4.1, 4.5, 5.4 or 6.5, as determined by isoelectric focusing. Pref. (I) hybridises to the insert of vector pMAN1 (in specification), and the yeast is pref. Saccharomyces cerevisiae or Trichoderma species. In hydrolysis, the glucomannans of cellulose pulp are hydrolysed by MN and pref. of at least 1 xylan. The MN treatment (pref. with xylanase) is carried out before bleaching (chlorine-free or peroxide) of sulphite pulp.
FI945266A 1992-05-22 1994-11-09 Mannanase enzyme and corresp. DNA - are produced in recombinant yeast or fungi; used for hydrolysis of manno-polymers, esp. in bleaching lignocellulose pulps FI118264B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FI945266A FI118264B (en) 1992-05-22 1994-11-09 Mannanase enzyme and corresp. DNA - are produced in recombinant yeast or fungi; used for hydrolysis of manno-polymers, esp. in bleaching lignocellulose pulps

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
FI922373 1992-05-22
FI922373A FI922373A0 (en) 1992-05-22 1992-05-22 MANNANASENZYMER, GENER SOM KODAR FOER DEM OCH FOERFARANDE FOER ISOLERING AV GENERNA SAMT FOERFARANDE FOER BLEKNING AV LIGNOCELLULOSAHALTIG MASSA.
FI931193 1993-03-17
FI931193A FI931193A0 (en) 1992-05-22 1993-03-17 MANNANASENZYMER, GENER SOM KODAR FOER DEM OCH FOERFARANDEN FOER ISOLERINGAV GENERNA SAMT FOERFARANDE FOER BLEKNING AV LIGNOCELLULOSAHALTIG MASSA
FI9300219 1993-05-24
PCT/FI1993/000219 WO1993024622A1 (en) 1992-05-22 1993-05-24 Mannanase enzymes, genes coding for them, methods for isolating the genes, and methods for bleaching lignocellulosic pulps
FI945266A FI118264B (en) 1992-05-22 1994-11-09 Mannanase enzyme and corresp. DNA - are produced in recombinant yeast or fungi; used for hydrolysis of manno-polymers, esp. in bleaching lignocellulose pulps
FI945266 1994-11-09

Publications (3)

Publication Number Publication Date
FI945266A0 FI945266A0 (en) 1994-11-09
FI945266A true FI945266A (en) 1994-11-09
FI118264B FI118264B (en) 2007-09-14

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Family Applications (1)

Application Number Title Priority Date Filing Date
FI945266A FI118264B (en) 1992-05-22 1994-11-09 Mannanase enzyme and corresp. DNA - are produced in recombinant yeast or fungi; used for hydrolysis of manno-polymers, esp. in bleaching lignocellulose pulps

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FI (1) FI118264B (en)

Also Published As

Publication number Publication date
FI118264B (en) 2007-09-14
FI945266A0 (en) 1994-11-09

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