FI59526B - ANALYSIS OF THE IDENTIFICATIONS - Google Patents

Description

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Patent- ocn registerstyrelsen AiwBku uthfd och uti. / 75 (71) Ewos Aktiebolag, Box 6l8, S-151 27 Södertälje, Sweden-Sweden (SE) (72) Roy Henry Smith, Reading, Berkshire, Alexander Baxter McAllan,

This invention relates to the use of certain ureide derivatives as ruminant feed materials and to a feed additive containing non-protein nitrogen (NPN nitrogen). Reading, Berkshire, England-England (GB) (7 * 0 Berggren Oy Ab (5I +) Use of urea in ruminant feed material - Användning av en ureid i idisslarfoder ) sources.

The object of the invention is to make it possible to feed ruminants with non-protein nitrogen in the form of urea and thus to avoid the formation of high concentrations of ammonia in the rumen and blood while providing a certain energy supplement.

Urine is widely used as a source of non-protein nitrogen in ruminant feed, but its use suffers from the significant disadvantage that ammonia is often formed in the rumen due to the microbial degradation effect faster than what can be utilized by rumen microorganisms and can rise to toxic concentrations in animals. In an effort to avoid this, NPN has been administered in forms in which the rate of ammonia formation is reduced, e.g., as a biuret, or urea has been co-administered with a urease inhibitor. These procedures reduce the risk of poisoning but do not generally lead to better utilization of ammonia by rumen microorganisms and, in fact, often reduce such utilization. The urea has also been physically combined with an energy source such as starch or molasses, which promotes microbial ammonia contact. However, the physical mixture may be initially inconsistent or may become inconsistent during storage, so that the supply of the energy source does not match the supply of ammonia and the risk of poisoning remains.

Mono-glucosyl urea has recently been administered to sheep in academic studies of bacterial metabolism of rumen, either mixed with diet or directly into the rumen. However, this substance is not economically viable as a ruminant feed material.

Compounds have now been found which eliminate the problems caused by the toxicity of ammonia and encourage the addition of ammonia to the microbial protein while providing an energy source for microbial synthesis and which can also be advantageously prepared from waste material.

The present invention thus comprises the use of a compound as a ruminant feed material or feed additive of general formula H 0

I II

R1-N-C-N-H2 with lactosyl.

The feed material or feed additive may contain one or more of these compounds or other compounds of formula or salts thereof, but may also contain other sources of non-protein nitrogen.

The feed materials and feed additives of the invention may be in any conventional non-protein nitrogen form. They may, for example, be in the form of a mixture containing lactose ureide with a suitable nutritional base, e.g. cereals, roots, fodder, silage fermented vegetable fodder, coarse fodder or industrial by-products such as bran, husks or energy-rich pulps or syrups, or mixtures thereof . The nutritional base preferably contains relatively little nitrogen but a lot of energy and is e.g. corn feed, sugar beet pulp or tapioca. The feed mixture may also contain minerals, vitamins and other nutritional additives such as, for example, supplementary fats and proteins. The invention encompasses compositions that do not contain any or only a minor portion of a nutritional base, and these compositions are usually in the form of feed material supplements and contain other ingredients such as supplementary nutritional additives in addition to the nitrogenous compound of the present invention.

Concentrations of non-protein nitrogen present in these mixtures may vary, e.g., according to methods of treatment of ruminants, taking into account e.g. factors such as the species, breed, sex, age and physiological condition of the animal and the proportion of the mixture in the total diet. For example, the nutritional nitrogen requirement of a beef cattle corresponding to a protein linkage of up to about 12% by weight in the diet may be replaced in whole or in part by non-protein nitrogen. The higher concentration of crude caprotein required by high-yielding dairy cows can be provided, at least in part, as a non-protein nitrogen, but preferably at least some of the correct protein must also be provided. Thus, the compositions of the present invention contain, for example, about 1 to 50% by weight or more of a nitrogenous compound of the invention, wherein in a preferred embodiment of the invention, 50% of the nutritional nitrogen is based on the nitrogenous compound of the invention. The amount administered to ruminants of the nitrogen-containing compounds of the invention will vary depending on whether or not other nitrogen sources are present, but may generally be administered at will if they constitute the sole nitrogen source.

Feed material mixtures can exist in a variety of physical forms, e.g., compressed, granulated, ground, natural, semi-liquid, or liquid form. The invention also encompasses feed additives, usually in compressed or granulated form, which may be put out to be fed to pasture animals to the extent desired and may also contain additives in liquid or semi-liquid form, e.g. in slurry form, e.g. for feeding to cows during milking. These additives may contain supplementary nutritional additives with or without a suitable nutritional base. However, the feed material is most commonly in the form of a compressed, granulated, ground or naturally structured feed material concentrate which is administered as part of a nutritional ration and which contains a suitable nutritional base.

The lactose compounds of the formula may be prepared by reacting a urea or a suitable derivative of a urea such as urea monophosphate, thiourea, guanidine, isobutylidene urea or (biuret) with lactose. The reaction is usually carried out with at least some of the reagents in solution, e.g. in aqueous solution, and, if desired, the compounds can be recovered in the form of solid products.

The reaction is carried out under acidic conditions and the reaction mixture can be heated. However, very acidic conditions and too high temperatures can lead to decomposition of the reagents.

A reaction mixture containing, for example, urea and lactose at 0.2 molar sulfuric acid and an incubation temperature of more than 50 ° C for 7 days has been found to be sufficient for the preparation of monolactosyl urea. The reaction mixture usually contains an excess of urea to lactose, e.g. a double excess of urea.

Dairy products can advantageously be used as a suitable source of lactose, and in particular any milk waste products, e.g. skimmed milk and in particular whey, which contains a relatively large proportion of lactose. Lactose ureas, for example, can be prepared by reacting whey with a urea. Whey or whey products and urea are preferably brewed together under acidic conditions and at an elevated temperature, e.g. at about 50 ° C.

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According to a preferred embodiment, the reaction is carried out using urinary and lactose-containing milk by-products in the form of unconcentrated whey, concentrated whey, whey permeate and / or buttermilk whey.

Unconcentrated whey is a waste product from cheese making in which whey contains about ^ 8? lactose and a total of about 8% dry matter.

Concentrated whey is a whey product from which water has been removed by evaporation and which contains about * 11% lactose and a total of about 62% dry matter.

Whey permeate is a product obtained from reverse osmosis of whey and contains about 50% lactose and a total of 65% dry matter.

Buttermilk-whey is a waste product obtained after making buttermilk protein concentrate from buttermilk. Dried buttermilk whey, which contains about 6% water, contains about 59% lactose.

The pH of the reaction mixture of urea and whey products should be kept between 1.5 and 3> 5> preferably at pH 2.

The temperature of the reaction mixture should be maintained above 40 ° C, but should not exceed about 80 ° C, with a preferred temperature of 60-70 ° C.

The reaction time must be long enough to produce a lactosylurea in a yield of at least 50%, which, depending on the concentration of whey used and the ratio of lactose to urea, can be up to * 1 day (96 hours).

The amount of urea present in the reaction mixture is at least equimolar, with a weight ratio of lactose to urea of 1: 0.15. However, the weight ratio can vary up to 1: 2.

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The acids used to oxidize the reaction mixture are selected from strong inorganic acids such as hydrochloric acid, sulfuric acid or phosphoric acid.

Thus, a process for preparing a urea to provide a ruminant feed material or feed additive comprises treating the whey with a urea or a suitable derivative of the urea to convert the lactose present in the whey to a compound or compounds of formula.

Suitable urea derivatives include, for example, urea monophosphate, thiourea, guanidine, isobutylidene urea or biuret. However, it is recommended that the urea be usually used in this reaction so that the product consists mainly of lactose ureides. The compounds of the formula can be separated from the impure product, e.g. by evaporation and recrystallization, and can subsequently be used for the preparation of feed materials and feed additives according to the invention. However, the product is preferably recovered in crude form, the solution is e.g. neutralized if desired, and the crude product can be concentrated, e.g. by evaporation or reverse osmosis, or recovered in solid form, e.g. by spray or roller drying. Such an impure product typically contains lactose ureas, e.g., monolactosyl urea, along with unreacted lactose and urea, as well as minor amounts of soluble milk proteins and other soluble ingredients. The crude product in solid, liquid or slurry form can be used directly as a ruminant feed additive or combined with other substances such as cereals, pulps, silage fermented vegetable feeds, coarse feeds, preferably supplements, oils, proteins, vitamins or other minerals.

The invention is described in more detail below by means of examples which describe the preparation of ruminant feed materials from whey.

Example 1

Urine (96 g) is added to two liters by running precipitated whey with 0.8 wt% lactose and the mixture is acidified by adding 90 ml of 5 5 t / v sulfuric acid. The reaction mixture is then incubated at 50 ° C for 7 days, after which at least 70% of the lactose 7 59526 is converted to ureide as determined by chromatography (m.p. 232-23 * 4 ° 0. The incubated reaction mixture is then neutralized by the addition of sodium hydroxide and the crude product is recovered on a solid. The ruminant feed material is then prepared by mixing the crude solid product with a suitable nutritional medium such as corn feed or sugar beet pulp, with a weight ratio of dry matter to crude product in the range of 1: 1 to 60: 1.

Alternatively, the crude product in solid form is used directly as a ruminant feed additive or the lactose ureas are separated and purified by evaporation of the reaction mixture and recrystallization from e.g. water and then used to prepare compound feeds.

Example 2

The same procedure as in Example 1 above is followed, except that the reaction mixture is incubated at 70 ° C for 15 hours, after which at least 50% of the lactose has reacted as lactosyl urea as determined chromatographically (m.p. 233-231 ° C).

Example 3

Urine (82 g) is added to 200 g of concentrated whey with 4 l of w / lactose and the mixture is acidified by adding 40 ml of $ 33 (v / v) sulfuric acid to pH 2. The reaction mixture is incubated at 70 ° C. 15 hours. The conversion of lactose to lactosyluride is chromatographically determined to be at least $ 85.

Example M

The same procedure as in Example 3 is followed, but using concentrated whey permeate with 50.2% by weight of lactose. The conversion of lactose to lactosylurea is at least 70% as determined by chromatography.

Example 5

The procedure is as in Example 3, except that H5 ml of concentrated sulfuric acid (1.75 specific gravity) are added to adjust the pH of the mixture to 2. The conversion of lactosyl to lactosyl uride is chromatographically determined to be at least 7H #.

Example 6

The same procedure as in Example 4 is followed, except that 60 ml of concentrated phosphoric acid (1.75 specific gravity) are added to adjust the pH of the mixture to 2. The conversion of lactose to lactosyl urea is at least 70% as determined by chromatography.

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Example 7

A nutritional concentrate for dairy cows was prepared. Cows producing about 30 kg of FCM per day (4 kg of fat) were given about 13 kg of concentrate per day. Such a daily dose contains the lactosyluride of Examples 1-6 0.7 kg of barley 6.0 "oats, rolled 5.5" beet pulp 0.5 "vitamins and minerals 0.3" Such a concentrate contains about 16% crude protein.

If the concentrate is supplemented with hay, 8 kg per day, a complete feed material for dairy cows is obtained.

Example 8 A complete feed material for calves for slaughter with a live weight of about 200 kg and a weight gain of 1000-1200 g per day contains hay and concentrate.

The composition of the daily dose of concentrate (5-6 kg) is as follows:

The lactosyl urea of Examples 1-6 0.180 kg barley 2.5 "oats, rolled 2.5" beet pulp 0.6 "vitamins and minerals 0.150" containing about 14% crude protein. The concentrate is supplemented with hay, 0.5 kg per animal per day.

The biological activity of the lactosylurea according to the invention was determined in a living organism and in a test tube as follows.

One sheep received a diet containing approximately equal parts of barley and straw, cubed, containing 15.7 g N / kg dry matter. Some of the cubes were then replaced with lactosylurea to provide nutritional doses of 25.4 g N / kg dry matter, 9,59526 of which half of the nitrogen content was derived from lactosylurea.

In the living organism: small samples of the contents of the rumen were removed and acidified at various times after feeding and ammonia levels were measured. Samples were taken when the sheep were fed 1) basic feed, 2) feed supplemented with lactosylurea, and 3) feed supplemented with equimolar amounts of lactose and urea.

Ammonia levels were measured during the pre-feeding and post-feeding periods. The pre-feeding period is the first 6 days of feeding the new feed material.

The ammonia concentrations obtained from the administration of feed materials 2 and 3 above are shown in Table 1 below.

table 1

Time of feeding Ammonia concentration in millimolar (hours) of kelp per liter of rumen lactosyl- Lactose + lyuride urea

Before Feeding After feeding After feeding 2. 5.> 19. 28. 34. 33-35 __day_day day day day day 0 12.5 15.8 17.5 14.4 16.6 11.4 0.5 14.3 16.1 16.2 13.8 15.1 30.2 1 15.2 15.4 18.4 14.6 14.7 42.5 2 19.9 15.4 18.3 17.6 15.1 57.8 4 20.5 16.3 23.3 18.3 18.4 31.0 6 19.2 18.9 37.4 48.7 29.2 23.9

As can be seen from the above, low ammonia levels are obtained 34 days after application using lactosyluride as a nitrogen source compared to the use of urea as a nitrogen source.

In the test tube, rumen contents were sampled 2 hours after feeding the basic feed and incubated anaerobically at 39 ° C for 1) lactosylurea (42.9 mmol / l) and 2) lactose (42.9 mmol / l) and urea (42, 9 mmol / l). Ammonia formation and urea loss were measured.

On the morning of the test glass test, lactosylurea was excluded from the diet with high N levels.

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Urinary loss was measured during the pre-feeding and post-feeding periods. The amounts of urea remaining in the incubated samples are given in Table 2 below.

Table 2

Time of feeding- Uranium concentration after nan mmol / l lactosyl, -1, + -__ „· ^ (hours) ureide

Before feeding Before feeding After feeding after Day 2 Day 18 Day 2 Day 20 0 40.6 47.7 42.2 43.7 0.5 39.6 45.8 40.2 33 .2 1 40.6 45.5 37.9 21.3 2 41.3 43.8 31.2 2.2 4 40.2 38.3 21.7 none 6 43.8 34.4 0.9 none

As shown in Table 2 above, the urea itself disappears very rapidly from the samples in contrast to the lactosylurea. This suggests that lactosylurea does not cause high levels of ammonia in the rumen.

In Table 2 above, urea loss was given. Table 3 below gives the formation of ammonia in the same test.

Table 3

Time of feeding - Ammonia concentration after nan (mmol / l) Lactose + urea (hours) lactosylurea

Before feeding Before feeding Before feeding After day 2 Day 18 Day 2 Day 20 0 None None None 0.5 0.7 0.5 None None None 7.9 20.5 1 No none 2.7 12.4 33.4 2 none 4.5 21.7 68.8 4 none 7.5 16.5 74.6 6 0.2 16.3 2.4 70.1

The conclusion drawn after Table 2 seems to be correct. Urine alone gives too high levels of ammonia in the rumen as opposed to lactosylurea.

11 59526 For example, an uptake of a fatty acid in the form of a fatty acid and a fatty acid of the fatty acid content of the fatty acid (NPN).

In particular, the use of uptake in the form of urea and proteinaceous form in the form of urea and in the form of ammonia concentrates in the form of energy is well controlled.

Urea is used in the production of certain protein proteins in the form of a mixture of ammonia from other producers and in the genomic microbiology of the micro-organism in which it is present in the micro-organism. is toxic to the skin. In the case of an NPN använts i former color ammonia products may not be exempted from biuret or urea from the same time as the urea inhibitor. This method of reducing the risk of toxicity is due to the presence of ammonia in the form of ammonia and the presence of non-toxic substances. Urea is also used in the former physical combination with energy-free starch or molasses, which is a microbiological form of ammonia. Compositions of the physical combination can be used as an initial or alternative to the energy of the energy source to produce ammonia and the risk of toxicity to the quaternary.

The Kingdom of Denmark is responsible for the administration of bacterial metabolism and the administration of monoglycosylureas to the Member States. This material is also available in an economically viable manner and is designed for use.

For example, it has been shown that there is a problem of ammonia toxicity and the presence of ammonia in the micro-biological protein genome as well as the use of energy for microbiological synthesis and in the production of micro-products.

I enlarge the size of the upper part of the upper part of the upper part of the upper part of the headlamp or the upper part of the headlamp form

H 0 H

1 i II

r, - n- c- n-h2 color is lactosyl.

Fodermedlet or fodertillsatsmedlet kan omfatta en eller bäda dessa föreningar eller andra föreningar enligt formeln eller deras salter men kan ocksä innehälla andra icke-proteinkvävekällor.

Fodermed and fatty acid-derived fatty acids can be used in the form of a single protein protein. Exempelvis can be used in the form of a composition in the form of lactose and lactose in the form of a mixture of cereals, rotfructer, forage, ensilage, grovfoder eller industri riella biprodukter säsom kli, scal eller energirik pulp eller saft eller blandning. Fördelaktigen har näringsgrundbland-ningen ett förhällandevis lägt kväveinnehäll men ett högt Energi-innehäll exempelvis majsensilage, sockerröravfall eller tapioka. Foderblandningen kan ocksä innehälla mineraler, vitamin och andra fodertillsatsmedel som exempelvis extra fettämnen och proteiner. The composition of the composition of the composition or of the composition of the composition or of the composition of the composition of the composition or of the composition of the composition of the composition of the composition is exempted from the additional cost.

Half of the protein proteins in the composition can be varied, the exceptions and the equivalents being determined by the method used in the present invention. tar sädana factorer i beaktande som vilken art som uppfodras, kön, aider och djurets physiologiska tillständ samt den relativa mängden av blandningen i den samlade kosten. Exempelvis kan det fodermässiga kvävebehovet för köttdjur svarande account ett proteinintag av upp account ca 12 vikt-% av kosten ersättas helt eller delvis av icke-proteininäväve, Den högre concentration icke-proteinkväve, men man föredrar att ocksä tillföra viss mängd dietiskt riktigt protein. 13 59526% of the composition up to about 50% by weight or up to 50% by weight of the compound up to 50% by weight of the compound feed in the form of a 50% dietary formula for the purposes of this Regulation. The playing field of the quarrying of the quarrying of the quarrying of the quarrying of the quarrying is carried out in such a way as to ensure that the quarrying is carried out.

Fodermedelkompositionerna kan föreligga inom ett stort omräde av fysiska former exempelvis i kompakterad, granulerad, pulveriserad, texturerad, halvflytande eller flytande form. The use of upstream feeds and feeders, such as compacts or granules in the form of light, is to be carried out in the form of a flat-rate or medium-sized feed in the form of an exemption from slurry. Dessa tillskottsfoder kan omfatta extra dietiska tillsatser med eller utan en warm food. The above-mentioned emellers are formulated in the form of compacts, granules, powders or naturally occurring textured dressing centers, for example in the form of fragrances and omniparticles.

Lactosulfurization of the urea monophosphate, thiourea, guanidine, isobutylidene, diurea or (biuret) in lactose is a chemical reaction of urea monohydrates, thiourea, guanidine, isobutylidene. For example, the genomic reaction is carried out with the reaction of the reactants and the reaction, the exemption is wattage, and the reaction is carried out in the form of a pure product.

Reaction genomics under Sura betingelser och reaktionsbland-ningen kan vämas. Emellertid kan mycket Sura betingelser och mycket höga temperaturer leda account degradering av reaktanterna.

Exempelvis can be used to react with the reaction of urea and lactose at a rate of 0.2 molar with an average of 50 minutes at incubation temperature of 50 ° C for a period of 7 days with the addition of monolactosylureas. There is a proven reaction to the urea and the urea is mediated by the account of lactose exemptions and that of the urea.

For the production of milk products other than those which are lactose and the synergistic potential of milk products 14 59526 exempted from milk products and special vassle, the light source is relatively stable. Exempelvis kan ureider av lactakt framställas genom reaktion av vassle med urea. The incubator is incubated under water or at a temperature between about 50 ° C and at room temperature.

Preferably, the formation of the genomic reaction by means of an urea and a lactose-containing product is in the form of concentrates, vats, vats or vermicles.

Okoncentrerad vassle är den avfallsprodukt som erhälls vid ostproducttion, vilken vassle innehäller about 4.8% lactose and totally ca 8% torrsubstans.

Concentrated vassle är en vassleprodukt, frän vilken vatten har avlägsnats genom indunstning och som innehäller ca 41% lactos and totalt ca 62% torrsubstans.

Vasslepermeat is a product that is present in a mixture of about 50% lactose and a total of about 65% torrmaterial.

Kärnmjölksvassle är en restprodukt erhällen efter att ha framställt ett kärnmjölksproteinkoncentrat frän kärnmjölk. Stabbed carnivorous beans about 6% lactated beans about 59% lactose.

The pH of the reaction mixture is urea and a pure product with a pH of 1.5 and 3.5, adjusted to pH 2.

The reaction temperature range is 40 ° C and the temperature is about 80 ° C, the temperature is 60-70 ° C.

Reaction scales with at least 50% of the lactosyluretic acid, up to a concentration of lactose and urea, can be up to 4 dagar (96 timmar).

I play urea in the nervous system and the reaction is not equimolar, the colors are very low: the urea is 1: 0.15. Vikt-förhällandet kan emellertid varieras upp account ett förhällande av 1: 2.

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The syrup may be used in connection with the surgical operation of the reaction group in the presence of starch from organic salts or in the presence of phosphorus.

Ureidframställningsförfarandet för Produktion av fodermedel eller fodertillsatsmedel för idisslare omfattar sälunda behandling av vassle med urea eller ett warming derivat av urea förnur urea, for example, a single-breasted lactose-free form of a white , guanidine, isobutylidene, diurea or biuret. For example, the above ureas may be used for genomic reactions to produce products of interest to lactose carriers. For example, the products may be separated from the products of the genome induction by crystallization, and may be used for the production of fodder and fodder uptake. For example, the above-mentioned products are used in the form of exemptions, the neutralization of which is neutralized by the use of osmosis, and in the case of products the concentration of exempted genomes is induced by the use of osmosis, or by the use of fast-release exemptions. In this case, the product obtained from the lactose purifier is exempted from monolactosylurea in the form of lactose and urea and oleate components. These products are fast, flour or slurry-shaped and can be used directly or in combination with other ingredients or in the composition of the material in which the material is exempt from cereals, pulp, ensilage, roufage, fertile-free, , vitamins or minerals.

The above illustrations do not include any exceptions for the use of the main body of the vehicle.

Exempt 1

Urea (96 g) was added to 2 liters of leachate in a mixture of 4.8% by weight of lactose and bleaching in the genome to 90 ml of 25% (v / v) solution. The reaction incubation was incubated at 50 ° C for 7 days after flashing from 70% lactose to 16.5526 parts of the chromatograph (mp 232-234 ° C). These incubation reactions neutralize the sedan genome with sodium hydroxide and give the product a fast form of genome spraying. That the fodermedel fits idarelare framställs sedan genom att blanda den orena fasta products with en warming of the brains on the ground or sockerbetspulp. The weight of the basic torr material is that the product is in a ratio of 1: 1 to 60: 1. Alternatively, these products may be used in a fast form for direct or indirect processing; or the lactose acid-containing and renal genomics are induced by reaction and recovery and are free from water and may be used for the preparation of the basic composition.

Exempel 2

Samples are then added to one of the skills of the reaction reaction incubator at 70 ° C under 15 times for at least 50% lactose and the lactosylurea is chromatographed (mp 233-234 ° C).

Exempel 3

Urea (82 g) was added to 200 g of concentrated water at 41% lactic acid and bleaching in the genome to 40 ml 33% (v / v) of pH 2. The reaction was incubated at 70 ° C under 15 ° C. The lactation of the lactosylurea is best chromatographed and is 85% or less.

Exempel 4

Samma förfarande feläs säsom i exempel 3 men med avvningning av concentrerad vasslepermeat innehällande 50,2 vikt-% lactos. The lactose content of lactose to lactosylureas is 70% chromatographic.

Exempel 5

Samples are then added up to 3 times with 45 ml of concentrated phosphorus (1.75 S.G.) and adjusted to pH 2. Bleaching to lactose accounts for at least 74% of the lactosylurea.

Exempel 6

The sample is then added to a mixture of 60 ml of concentrated phosphorus (1.75 S.G.) at a pH of 17 59526 and 2. The addition of lactose to the lactosylurea is carried out with a chromatographic solution of at least 70%.

Exempel 7

This foderkoncentrat avsett för mjölkkor framsställdes. The product yields about 30 kg of milk per portion (4 kg of fat) and about 13 kg of concentrate per portion. In addition to the French lactone intact lactosylurea, one to six copies of 1 to 6 0,7 kg of 6,0 '' coffee, prepared in 5,5 "

The precipitate is concentrated to about 16% rSprotein.

Qm concentrate is complete with heat, 8 kg per dose, and is used as a complete powder for xnjölkkor.

Exempel 8

This complete set of frying pans is provided with a weight of about 200 kg and a weight of 1000-1200 g per portion of heat and concentrate.

The composition of the active ingredient in the concentrate (5-6 kg) is as follows: lactosylurea with an exemplary content of 1-6 0.180 kg rag 2.5 Havre, anthers 2.5 "betpulp 0.6 vitamins and minerals 0.150" to run a report. Concentrate complete with heat, 0.5 kg per djur and dag.

The biological effects of lactosylureas are preferably up to * and in vivo in vivo and in vivo.

The dietary weight of the dietary mixture is reduced to 15.7 g N / kg of torrsubstans. In the case of lactose silylureas, the dietary acid content is 25.4 g N / kg of torrsubstans, and the quaternary acid content of the lactosylurea.

18 59526

In vivo: Smä prov av vomvätska avlägsnades och surgjordes vid olika tidpunkter efter utfodring och ammoniakkoncentrationerna mättes. For the purposes of the present invention 1) med basfodermedel, 2) fodermedel supplementerat med lactactylurea och 3) fodermedel försett med equimolära mängder av lactakt och urea.

Ammoniakkoncentrationerna mättes under förutfodringsperioder samt efterutfodringsperioder. The period of validity of the period is 6 years from the date of entry into service.

Ammonia concentrations in the fodder of the feedstock 2) and 3) are shown in Table 1 below.

Table 1

Tid efter Kone. of NH 4 (mM) / 1 vomvätska utfodring Lactosylurea Lactos + urea 'limnar' förutfodr. eftutfodring efterutfodring 2: a dagen 5: e 19: e 28: e 34: e dagen 33-35: e dagen 0 12.5 15.8 17.5 14.4 16.6 11.4 0.5 14.3 16.1 16.2 13.8 15.1 30.2 1 15.2 15.4 18.4 14.6 14.7 42.5 2 19.9 15.4 18.3 17.6 15.1 57.8 4 20.5 16.3 23.8 18.3 18.4 31.0 6 19.2 18.9 37.4 48.7 29.2 23.9

The ammonia concentration in the ammonia concentration after 34 days is determined by the addition of lactosylureas to the quaternary residue and the addition of urea to the quaternary quaternary.

An in vitro test can be performed at 2 ° C or after incubation at basal and incubated at 39 ° C with 1) lactosylurea (42.9 mM / Ι) and 2) lactose (42.9 mM / Ι) and urea ( 42.9 mM / Ι). Ammonia production and urea effluents.

The in vitro test for lactosylureas is carried out on dietary animals.

Ureaförlusten mättes under förutfodringsperioden respektive efter-utfodringsperioden. Kvarvarande mäng urea i de incuberade proverna ges i tabell 2 nedan.

19 59526

Table 2

Tid efter Kone. urea (mM / 1) utfodring Lactosylurea Lactos + urea (timmar) förutfodring efterutfodring förutfodring efterutfodring 2: dagen 18: e dagen 2: dagen 20: e dagen 0 40.6 47.7 42.2 43.7 0.5 39.6 45.8 40.2 33.2 1 40.6 45.5 37.9 21.3 2 41.3 43.8 31.2 2.2 4 40.2 38.3 21.7 shirts 6 43.8 34.4 0.9 shirt

In the case of Table 2, the first urea ensatm mycket snabbt frän proverna varvid lactosylurean inte gör det. This is indicated by the addition of lactosylureas in the form of NH 4 -nails.

I tabell 2 ovan har ureaförlusten givits. Table 3 shows the ammonia production and the test.

Table 3

Tid efter Kone. NH 2 (mM / 1) utfodring Lactosylurea Lactos + urea (timmar) förutfodring efterutfodring förutfodring efterutfodring 2: a dagen 18: e dagen 2: a dagen 20: e dagen 0 inget inget 0,5 0,7 0,5 inget inget 7 .9 20.5 1 shirt 2.7 12.4 33.4 2 shirt 4.5 21.7 68.8 4 shirt 7.5 16.5 74.6 6 0.2 16.3 2.4 70 1

The slutsats are drugs ur table 2 tycks copper riktig. Urea first is commercially available in the form of Nl-concentration, and lactan silylates are commercially available.