FI20060533L - Enhanced expression of tuberculosis vaccine proteins in plants - Google Patents

Enhanced expression of tuberculosis vaccine proteins in plants Download PDF

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Publication number
FI20060533L
FI20060533L FI20060533A FI20060533A FI20060533L FI 20060533 L FI20060533 L FI 20060533L FI 20060533 A FI20060533 A FI 20060533A FI 20060533 A FI20060533 A FI 20060533A FI 20060533 L FI20060533 L FI 20060533L
Authority
FI
Finland
Prior art keywords
proteins
tuberculosis vaccine
plants
enhanced expression
vaccine proteins
Prior art date
Application number
FI20060533A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI20060533A0 (en
Inventor
Yuri Leonidovich Dorokhov
Timo Kalevi Korpela
Joseph Grigorievich Atabekov
Original Assignee
Timo Kalevi Korpela
Yuri Leonidovich Dorokhov
Joseph Grigorievich Atabekov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Timo Kalevi Korpela, Yuri Leonidovich Dorokhov, Joseph Grigorievich Atabekov filed Critical Timo Kalevi Korpela
Priority to FI20060533A priority Critical patent/FI20060533L/en
Publication of FI20060533A0 publication Critical patent/FI20060533A0/en
Priority to PCT/FI2007/000147 priority patent/WO2007138155A1/en
Publication of FI20060533L publication Critical patent/FI20060533L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

Abstract

The present invention provides constructs and methods for expression and accumulation of tuberculosis vaccine proteins in tissue or organ of a plant. The proteins are obtained by transient expression in and/or stable transformation of plants with non-replicating genes or virus-based vector cDNAs encoding the proteins. The genes include cellular compartment-specific, regulatory and/or affinity purification tag sequences. The plant tissue fractions containing the tuberculosis vaccine proteins themselves or after purification are useful as a tuberculosis vaccine.
FI20060533A 2006-06-01 2006-06-01 Enhanced expression of tuberculosis vaccine proteins in plants FI20060533L (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FI20060533A FI20060533L (en) 2006-06-01 2006-06-01 Enhanced expression of tuberculosis vaccine proteins in plants
PCT/FI2007/000147 WO2007138155A1 (en) 2006-06-01 2007-05-30 Improved expression of tuberculosis vaccine proteins in plants

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI20060533A FI20060533L (en) 2006-06-01 2006-06-01 Enhanced expression of tuberculosis vaccine proteins in plants

Publications (2)

Publication Number Publication Date
FI20060533A0 FI20060533A0 (en) 2006-06-01
FI20060533L true FI20060533L (en) 2007-12-02

Family

ID=36651361

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20060533A FI20060533L (en) 2006-06-01 2006-06-01 Enhanced expression of tuberculosis vaccine proteins in plants

Country Status (2)

Country Link
FI (1) FI20060533L (en)
WO (1) WO2007138155A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1394596B1 (en) * 2008-09-30 2012-07-05 Fond Parco Tecnologico Padano PRODUCTION OF NGF IN PLANT.
CN101845456B (en) * 2010-03-31 2012-11-21 暨南大学 Corn expression vector of tubercle Ag85B gene and application thereof
US20150196627A1 (en) * 2011-10-24 2015-07-16 University Of Central Florida Research Foundation, Inc. Plastid-expressed mycobacterium tuberculosis vaccine antigens esat-6 and mtb72f fused to cholera toxin b subunit

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10143205A1 (en) * 2001-09-04 2003-03-20 Icon Genetics Ag Process for protein production in plants
DE10321963A1 (en) * 2003-05-15 2004-12-02 Icon Genetics Ag Process for the production of a plastid-directed protein in plant cells

Also Published As

Publication number Publication date
FI20060533A0 (en) 2006-06-01
WO2007138155A1 (en) 2007-12-06

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