FI20041124A - Selection marker gene free hybrid strains, process for their preparation, and use of these strains - Google Patents

Selection marker gene free hybrid strains, process for their preparation, and use of these strains Download PDF

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Publication number
FI20041124A
FI20041124A FI20041124A FI20041124A FI20041124A FI 20041124 A FI20041124 A FI 20041124A FI 20041124 A FI20041124 A FI 20041124A FI 20041124 A FI20041124 A FI 20041124A FI 20041124 A FI20041124 A FI 20041124A
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FI
Finland
Prior art keywords
strains
selection marker
preparation
marker gene
dna
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FI20041124A
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Finnish (fi)
Swedish (sv)
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FI117388B (en
Inventor
Gerardus Cornelis Maria Selten
Bart Willem Swinkels
Gorcom Robertus Franciscus Van
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Dsm Ip Assets Bv
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Publication of FI20041124A publication Critical patent/FI20041124A/en
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Publication of FI117388B publication Critical patent/FI117388B/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

Abstract

The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
FI20041124A 1993-07-23 2004-08-27 Selection marker gene free yeast strains, process for their preparation and use of these strains FI117388B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93202195 1993-07-23
EP93202195 1993-07-23

Publications (2)

Publication Number Publication Date
FI20041124A true FI20041124A (en) 2004-08-27
FI117388B FI117388B (en) 2006-09-29

Family

ID=8214006

Family Applications (2)

Application Number Title Priority Date Filing Date
FI943485A FI114481B (en) 1993-07-23 1994-07-22 Selection marker gene-free filamentous fungal strains, process for their preparation, and use of these strains
FI20041124A FI117388B (en) 1993-07-23 2004-08-27 Selection marker gene free yeast strains, process for their preparation and use of these strains

Family Applications Before (1)

Application Number Title Priority Date Filing Date
FI943485A FI114481B (en) 1993-07-23 1994-07-22 Selection marker gene-free filamentous fungal strains, process for their preparation, and use of these strains

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US (3) US5876988A (en)
EP (2) EP1321523A3 (en)
JP (2) JP3833725B2 (en)
CN (1) CN100347301C (en)
AT (1) ATE238425T1 (en)
BR (1) BR9402912A (en)
CA (1) CA2128589C (en)
DE (1) DE69432543T2 (en)
DK (1) DK0635574T3 (en)
ES (1) ES2198410T3 (en)
FI (2) FI114481B (en)
IE (1) IE20030875A1 (en)
NZ (2) NZ299139A (en)

Families Citing this family (168)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1003096C2 (en) 1995-05-15 1996-11-19 Gist Brocades Bv Application of phospholipases in animal feed.
ATE380242T1 (en) 1995-06-23 2007-12-15 Danisco NUCLIC ACID SEQUENCE XLNR OF THE REGULATOR XYLR OF THE XYLANOLYTIC BIOSYNTHESIS PATHWAY FROM ASPERGILLUS NIGER
ATE418614T1 (en) 1995-08-03 2009-01-15 Dsm Ip Assets Bv AMDS GENE FROM ASPERGILLUS NIGER CODING FOR AN ACETAMIDASE
DK0814165T3 (en) * 1996-06-21 2003-07-21 Suntory Ltd Method for constructing transformed yeast cells that do not contain a selectable marker gene
AU7642298A (en) 1997-04-11 1998-11-11 Gist-Brocades B.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
JP4531868B2 (en) * 1997-04-11 2010-08-25 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ Yeast cells, particularly by Kluyveromyces, comprising at least two copies of the desired gene integrated in the chromosomal genome of the domain encoding more than one non-ribosomal RNA
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
US6558937B1 (en) 1997-07-31 2003-05-06 Dsm N.V. Cellulose degrading enzymes of aspergillus
CN1284998A (en) 1997-12-22 2001-02-21 尤尼利弗公司 A process for site-directed integration of multiple copies of gene in mould
DK1042461T3 (en) * 1997-12-22 2007-09-24 Dsm Ip Assets Bv Expression cloning in filiform fungi
MXPA00011223A (en) 1998-05-19 2002-04-17 Dsm Nv Improved in vivo.
US6358705B1 (en) 1998-07-16 2002-03-19 Novo Nordisk A/S Method of making proteins in transformed yeast cells
EP1097228B1 (en) * 1998-07-16 2006-03-01 Novo Nordisk A/S Method of making proteins in transformed yeast cells
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
US6750379B2 (en) 2000-03-09 2004-06-15 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
DK1319079T3 (en) 2000-09-21 2013-01-07 Basf Se Talaromyces xylanase
ES2319757T5 (en) 2002-01-23 2018-05-22 Dsm Ip Assets B.V. Fermentation of pentose sugars
US7517671B2 (en) 2002-02-28 2009-04-14 New England Biolabs, Inc. Methods and compositions for concentrating secreted recombinant protein
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
AU2003285818A1 (en) * 2002-11-28 2004-06-18 Universiteit Leiden A method for marker-less integration of a sequence of interest into the genome of a cell
KR20060061295A (en) 2003-05-28 2006-06-07 디에스엠 아이피 어셋츠 비.브이. Cephem compound
CN1298213C (en) * 2003-09-10 2007-02-07 王海波 Technology of obtaining gene transferred plant without selecting mark through division process
WO2005040186A2 (en) * 2003-10-14 2005-05-06 Dsm Ip Assets B.V. Method for preparing a modified host cell
EP3351637B1 (en) 2004-04-02 2020-06-10 DSM IP Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency
CN102286483B (en) 2004-04-16 2014-06-04 中化帝斯曼制药有限公司荷兰公司 Fungal promoters for expressing a gene in a fungal cell
WO2006040358A2 (en) * 2004-10-15 2006-04-20 Dsm Ip Assets B.V. Homologous amds genes as selectable marker
WO2006078164A2 (en) 2005-01-18 2006-07-27 Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patientenzorg Mycobacteria with mannose cap-deficient lipoarabinomannan
CA2595056A1 (en) 2005-01-24 2006-07-27 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
US20090176219A1 (en) 2005-11-29 2009-07-09 Lucie Parenicova Dna binding site of a transcriptional activator useful in gene expression
US8637280B2 (en) 2005-12-01 2014-01-28 Adcuram Nutrition Holding Gmbh Genes useful for the industrial production of citric acid
EP2035561A1 (en) 2006-06-29 2009-03-18 DSMIP Assets B.V. A method for achieving improved polypeptide expression
EP2082046A2 (en) 2006-11-02 2009-07-29 DSMIP Assets B.V. Improved production of secreted proteins by filamentous fungi
AU2008214663B2 (en) 2007-02-15 2013-10-03 Dsm Ip Assets B.V. A recombinant host cell for the production of a compound of interest
US20100120154A1 (en) 2007-03-21 2010-05-13 Dsm Ip Assets B.V. Method for homologous recombination
WO2009065777A1 (en) 2007-11-20 2009-05-28 Dsm Ip Assets B.V. Dicarboxylic acid production in a filamentous fungus
EP2220232B1 (en) 2007-11-20 2014-09-03 DSM IP Assets B.V. Succinic acid production in a eukaryotic cell
BRPI0819717B1 (en) 2007-11-20 2022-05-17 Dsm Ip Assets B.V. Recombinant yeast and process for the production of succinic acid
MX2010006679A (en) 2007-12-17 2010-11-30 Univ Amsterdam Light-driven co2 reduction to organic compounds to serve as fuels or as industrial half products by an autotroph containing a fermentative gene cassette.
FR2927089B1 (en) 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra METHOD OF TARGETED INTEGRATION OF MULTICOPIES OF A GENE OF INTEREST IN A YARROWIA STRAIN
EP2116136A1 (en) 2008-05-08 2009-11-11 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Novel phytases
DK2310517T3 (en) * 2008-07-11 2016-06-06 Inst Nat De La Rech Agronomique (Inra) Novel mutant yeast strains having a capability to accumulate a large amount of lipids
US20110294191A1 (en) * 2008-07-29 2011-12-01 Danisco Us Inc. Increased production of aspartic proteases in filamentous fungal cells
EP2392649A3 (en) 2008-08-05 2012-01-11 DSM IP Assets B.V. Adipoyl-7-ADCA producing strains
CA2754387C (en) 2009-03-10 2018-11-13 Dsm Ip Assets B.V. Pregastric esterase and derivatives thereof
WO2010102982A1 (en) 2009-03-10 2010-09-16 Dsm Ip Assets B.V. Method for improving the yield of a polypeptide
US8735100B2 (en) 2009-03-17 2014-05-27 Nederlandse Organisatie Voor Toegepast -Natuurwetenschappelijk Onderzoek Tno Cellulose and ligno-cellulose active proteins
WO2010118932A1 (en) 2009-04-15 2010-10-21 Dsm Ip Assets B.V. Dicarboxylic acid production process
CA2758404A1 (en) 2009-04-22 2010-10-28 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
BRPI1015536A2 (en) 2009-04-24 2019-07-02 Dsm Ip Assets Bv carbohydrate degradation polypeptide and uses thereof
UA108853C2 (en) 2009-07-10 2015-06-25 Galactose fermentation method
WO2011009700A1 (en) 2009-07-22 2011-01-27 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
DK2491120T3 (en) 2009-10-22 2016-03-21 Basf Se Synthetic phytasevarianter
EP2496686A1 (en) 2009-11-04 2012-09-12 DSM IP Assets B.V. Talaromyces transformants
US20130011876A1 (en) 2010-02-04 2013-01-10 Dsm Ip Assetts, B.V. Process for preparing filamentous fungal strains having a sexual cycle and a process for preparing sexually crossed filamentous fungal strains
EA201201125A1 (en) 2010-02-11 2013-03-29 ДСМ АйПи АССЕТС Б.В. CELL-HOST, THE ABLE TO PRODUCT ENZYMES SUITABLE FOR DEGRADATION OF LIGNOCELLULOSE MATERIAL
WO2011098580A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
MX341905B (en) 2010-04-21 2016-09-06 Dsm Ip Assets B V * Cell suitable for fermentation of a mixed sugar composition.
EP2388331A1 (en) 2010-05-21 2011-11-23 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Constitutive promoter
AU2011273684B2 (en) 2010-06-29 2014-08-28 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
EA201300059A1 (en) 2010-06-29 2013-05-30 ДСМ АйПи АССЕТС Б.В. POLYPEPTID, possessing the activity of the Volvolenin, and its application
US9109213B2 (en) 2010-06-29 2015-08-18 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
US9441214B2 (en) 2010-06-29 2016-09-13 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
ES2628345T3 (en) 2010-06-29 2017-08-02 Dsm Ip Assets B.V. Polypeptide that has or that helps in the degradation activity of glycidic materials and uses thereof
EA201300056A1 (en) 2010-06-29 2013-07-30 ДСМ АйПи АССЕТС Б.В. POLYPEPTIDE WITH ACTHYLXYLANESTERASE ACTIVITY AND ITS APPLICATIONS
CA2803222A1 (en) 2010-07-01 2012-01-05 Dsm Ip Assets B.V. A method for the production of a compound of interest
KR20130100169A (en) 2010-10-13 2013-09-09 디에스엠 아이피 어셋츠 비.브이. Pentose and glucose fermenting yeast cell
CN103596970A (en) 2011-03-11 2014-02-19 帝斯曼知识产权资产管理有限公司 Vector-host system
HUE027001T2 (en) 2011-04-21 2016-10-28 Basf Se Synthetic phytase variants
CN103502267A (en) 2011-04-22 2014-01-08 帝斯曼知识产权资产管理有限公司 Yeast cell capable of converting sugars including arabinose and xylose
EP2554668A1 (en) 2011-08-04 2013-02-06 DSM IP Assets B.V. A pentose sugar fermenting cell
AR087423A1 (en) 2011-08-04 2014-03-19 Dsm Ip Assets Bv CAPABLE CELL TO FERMENT PENTOUS SUGARS
US20150031868A1 (en) 2012-01-23 2015-01-29 Dsm Ip Assets B.V. Diterpene production
WO2013189878A1 (en) 2012-06-19 2013-12-27 Dsm Ip Assets B.V. Promoters for expressing a gene in a cell
DK2875119T3 (en) 2012-07-19 2018-04-03 Dsm Ip Assets Bv AGSE-DEFICIENT TRIALS
KR20150070151A (en) 2012-10-16 2015-06-24 디에스엠 아이피 어셋츠 비.브이. Cells with improved pentose conversion
WO2014096436A1 (en) * 2012-12-21 2014-06-26 Estibio Aps Marker-free genetically-engineered double mutants of thermoanaerobacter
DK3202900T3 (en) 2013-02-04 2019-04-08 Dsm Ip Assets Bv CARBOHYDRATE DEGRADING POLYPEPTIDE AND APPLICATIONS THEREOF
EP2772545A1 (en) 2013-03-01 2014-09-03 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Starch active proteins
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
US20160060660A1 (en) 2013-04-05 2016-03-03 Université Du Luxembourg Biotechnological production of itaconic acid
CN105431539B (en) 2013-05-02 2021-05-25 乐斯福公司 Novel organic acid pathway
US10689681B2 (en) 2013-05-31 2020-06-23 Dsm Ip Assets B.V. Microorganisms for diterpene production
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
AU2014292150B2 (en) 2013-07-15 2019-04-04 Dsm Ip Assets B.V. Diterpene production
EP3536697A1 (en) 2013-07-31 2019-09-11 DSM IP Assets B.V. Recovery of steviol glycosides
EP3077517B1 (en) 2013-12-02 2018-10-17 DSM IP Assets B.V. Ice structuring protein
WO2016012429A1 (en) 2014-07-24 2016-01-28 Dsm Ip Assets B.V. Yeast cell with improved pentose transport
FR3028527A1 (en) 2014-11-13 2016-05-20 Pivert IDENTIFICATION OF TRANSCRIPTION FACTORS OF YARROWIA LIPOLYTICA
DK3242950T3 (en) 2015-01-06 2021-12-20 Dsm Ip Assets Bv CRISPR-CAS SYSTEM FOR A WIRED MUSHROOM MUSHROOM HOST CELL
US10619170B2 (en) 2015-01-06 2020-04-14 Dsm Ip Assets B.V. CRISPR-CAS system for a yeast host cell
EP3242948B1 (en) 2015-01-06 2021-12-22 DSM IP Assets B.V. A crispr-cas system for a yarrowia host cell
WO2016127083A1 (en) 2015-02-06 2016-08-11 Cargill, Incorporated Modified glucoamylase enzymes and yeast strains having enhanced bioproduct production
CN107429273A (en) 2015-02-16 2017-12-01 帝斯曼知识产权资产管理有限公司 Method for producing itaconic acid under anaerobic
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
WO2016151046A1 (en) 2015-03-23 2016-09-29 Dsm Ip Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
EP3274461A1 (en) 2015-03-27 2018-01-31 Cargill, Incorporated Glucoamylase-modified yeast strains and methods for bioproduct production
BR112017021066B1 (en) 2015-04-03 2022-02-08 Dsm Ip Assets B.V. STEVIOL GLYCOSIDES, METHOD FOR THE PRODUCTION OF A STEVIOL GLYCOSIDE, COMPOSITION, RELATED USES, FOOD, PET FOOD AND BEVERAGE
CA2980649A1 (en) 2015-04-21 2016-10-27 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthase
EP3298133B1 (en) 2015-05-22 2019-09-25 DSM IP Assets B.V. Acetate consuming yeast cell
NZ737305A (en) 2015-06-02 2021-07-30 Dsm Ip Assets Bv Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
EP4043579A1 (en) 2015-07-10 2022-08-17 DSM IP Assets B.V. Method for preparing a steviol glycoside compostion
EP3332004A1 (en) 2015-08-05 2018-06-13 Cargill, Incorporated Xylose isomerase-modified yeast strains and methods for bioproduct production
WO2017025649A1 (en) 2015-08-13 2017-02-16 Dsm Ip Assets B.V. Steviol glycoside transport
CN108368490A (en) 2015-09-21 2018-08-03 普拉克生化公司 Fungi generates FDCA
WO2017050652A1 (en) 2015-09-25 2017-03-30 Dsm Ip Assets B.V. Asparaginase
CA2999638A1 (en) 2015-10-05 2017-04-13 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
EP3359655B1 (en) 2015-10-06 2019-11-20 DSM IP Assets B.V. Eukaryotic cell with increased production of fermentation product
BR112018010384A2 (en) 2015-11-24 2018-12-04 Cargill Inc genetically modified yeast and process for making a fermentation product
WO2017093450A1 (en) 2015-12-02 2017-06-08 Basf Se Method of producing proteins in filamentous fungi with decreased clr2 activity
BR112018011503A2 (en) 2015-12-07 2018-12-04 Zymergen Inc corynebacterium glutamicum promoters
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
KR20190090081A (en) 2015-12-07 2019-07-31 지머젠 인코포레이티드 Microbial Strain Improvement by a HTP Genomic Engineering Platform
BR112018013385A2 (en) 2015-12-30 2018-12-04 Dsm Ip Assets Bv partial enzymatic hydrolysis of triglycerides
WO2017115323A2 (en) 2015-12-30 2017-07-06 Dsm Ip Assets B.V. Partial enzymatic hydrolysis of triacylglycerols
WO2017167849A1 (en) 2016-03-31 2017-10-05 Dsm Ip Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
WO2017167848A1 (en) 2016-03-31 2017-10-05 Dsm Ip Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
EP3435770A1 (en) 2016-03-31 2019-02-06 DSM IP Assets B.V. Production of milk with low lactose content
CA3027448A1 (en) 2016-06-14 2017-12-21 Dsm Ip Assets B.V. Recombinant yeast cell
WO2018005655A2 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
JP2019519241A (en) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド Method for producing a glucose permease library and its use
US10787649B2 (en) 2016-07-13 2020-09-29 Dsm Ip Assets B.V. Malate dehyrogenases
US10913938B2 (en) 2016-07-29 2021-02-09 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
CN109661403A (en) 2016-08-05 2019-04-19 嘉吉公司 The yeast strain for the engineering that the glucoamylase polypeptide of leader sequence modification and the biologic with enhancing generate
EP3497110A1 (en) 2016-08-09 2019-06-19 DSM IP Assets B.V. Crystallization of steviol glycosides
EP3497109A1 (en) 2016-08-09 2019-06-19 DSM IP Assets B.V. Crystallization of steviol glycosides
EP3504336A1 (en) 2016-08-26 2019-07-03 Lesaffre et Compagnie Improved production of itaconic acid
WO2018073107A1 (en) 2016-10-19 2018-04-26 Dsm Ip Assets B.V. Eukaryotic cell comprising xylose isomerase
CA3040585A1 (en) 2016-10-27 2018-05-03 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthases
CA3045722A1 (en) 2016-12-08 2018-06-14 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
BR112019012559A2 (en) 2016-12-21 2019-11-26 Dsm Ip Assets Bv lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
EP3559222A1 (en) 2016-12-21 2019-10-30 DSM IP Assets B.V. Lipolytic enzyme variants
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
CA3048658A1 (en) * 2016-12-30 2018-07-05 Zymergen Inc. A method to build fungal production strains using automated steps for genetic manipulation and strain purification
US20200063166A1 (en) 2017-03-13 2020-02-27 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
EP3378931A1 (en) 2017-03-21 2018-09-26 Purac Biochem B.V. Fdca-decarboxylating monooxygenase-deficient host cells for producing fdca
EP3635111A1 (en) 2017-06-06 2020-04-15 Zymergen, Inc. High throughput transposon mutagenesis
EP3635112A2 (en) 2017-06-06 2020-04-15 Zymergen, Inc. A htp genomic engineering platform for improving fungal strains
BR112019025777A2 (en) 2017-06-13 2020-06-30 Dsm Ip Assets B.V. recombinant yeast cell
EP4155400A1 (en) 2017-06-27 2023-03-29 DSM IP Assets B.V. Udp-glycosyltransferases
CA3074001A1 (en) 2017-09-26 2019-04-04 Dsm Ip Assets B.V. Acetic acid consuming strain
EP3533878A1 (en) 2018-02-28 2019-09-04 Dutch DNA Biotech B.V. Process for producing citramalic acid employing aspergillus
UA126992C2 (en) 2018-05-07 2023-03-01 Дсм Айпі Асетс Б.В. Process for enzymatic oil degumming
KR20210018219A (en) 2018-06-06 2021-02-17 지머젠 인코포레이티드 Modification of genes involved in signaling to control fungal morphology during fermentation and production
EP3809852A1 (en) 2018-06-19 2021-04-28 DSM IP Assets B.V. Lipolytic enzyme variants
US20220389458A1 (en) 2019-11-04 2022-12-08 Dsm Ip Assets B.V. Low volume transfection
JP2023511858A (en) 2020-01-14 2023-03-23 ザ プロテイン ブルワリー ビー．ヴイ． Expression of ovalbumin and its natural variants
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
EP4232568A1 (en) 2020-10-22 2023-08-30 DSM IP Assets B.V. Microorganisms for diterpene production
EP4023757A1 (en) 2020-12-29 2022-07-06 The Protein Brewery B.V. Methods for generating selection marker-free transformants of heterokaryotic organisms
AU2022297881A1 (en) 2021-06-24 2024-01-18 Fonterra Co-Operative Group Limited Recombinant beta-lactoglobulin
WO2023285297A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
CN117916381A (en) 2021-07-12 2024-04-19 丹尼斯科美国公司 Recombinant yeast cells
WO2023285282A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023285294A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023285280A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023285281A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023079049A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Variant polypeptide and recombinant yeast cell
WO2023079050A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023079048A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Process for the production of ethanol and recombinant yeast cell
WO2023086793A1 (en) 2021-11-09 2023-05-19 Amgen Inc. Production of therapeutic proteins
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2023242273A1 (en) 2022-06-14 2023-12-21 Newmilkbuzz B.V. Expression of milk caseins in filamentous fungi

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422267A (en) * 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
EP0625577A1 (en) * 1985-08-29 1994-11-23 Genencor International, Inc. Heterologous polypeptides expressed in filamentous fungi, processes for their preparation, and vectors for their preparation
US5298405A (en) * 1986-04-30 1994-03-29 Alko Limited Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production
US5079145A (en) * 1988-10-21 1992-01-07 Synergen Associates, Inc. Process for preparing microorganisms used for making phenyl acetyl carlinol (pac)
GB8904762D0 (en) * 1989-03-02 1989-04-12 Glaxo Group Ltd Biological process
US5695976A (en) * 1989-12-18 1997-12-09 Novo Nordisk A/S Stable integration of DNA in bacterial genomes
US5498830A (en) * 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5824513A (en) * 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
EP0498137A1 (en) * 1991-02-06 1992-08-12 Novo Nordisk A/S Novel expression systems
WO1993001283A1 (en) * 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
EP0565172A1 (en) * 1992-04-10 1993-10-13 Quest International B.V. Cloning and expression of DNA encoding a ripening form of a polypeptide having sulfhydryl oxidase activity
US5550045A (en) * 1992-05-15 1996-08-27 Unilever Patent Holdings, B.V. Cloning and expression of DNA encoding a ripening form of a polypeptide having rhamnogalcturonase activity
US6291205B1 (en) * 1992-06-12 2001-09-18 Merck & Co., Inc. Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
US6110713A (en) * 1993-03-23 2000-08-29 Regents Of The University Of Minnesota Production of glutamic acid and lysine using auxotrophic mutants of Bacillus methanolicus

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