FI20001584A - Menetelmõ geenien transfektoimiseksi kationisten polymeerien ja lipopolyamiinien avulla - Google Patents

Menetelmõ geenien transfektoimiseksi kationisten polymeerien ja lipopolyamiinien avulla Download PDF

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Publication number
FI20001584A
FI20001584A FI20001584A FI20001584A FI20001584A FI 20001584 A FI20001584 A FI 20001584A FI 20001584 A FI20001584 A FI 20001584A FI 20001584 A FI20001584 A FI 20001584A FI 20001584 A FI20001584 A FI 20001584A
Authority
FI
Finland
Prior art keywords
lipopolyamine
cationic polymers
gene transfection
transfection
gene
Prior art date
Application number
FI20001584A
Other languages
English (en)
Swedish (sv)
Other versions
FI20001584A0 (fi
FI111014B (fi
Inventor
Atso Raasmaja
Pasi Lampela
Pekka T Monnist
Original Assignee
Atso Raasmaja
Pekka T Monnist
Pasi Lampela
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Atso Raasmaja, Pekka T Monnist, Pasi Lampela filed Critical Atso Raasmaja
Priority to FI20001584A priority Critical patent/FI111014B/fi
Publication of FI20001584A0 publication Critical patent/FI20001584A0/fi
Priority to US09/987,156 priority patent/US20020031502A1/en
Publication of FI20001584A publication Critical patent/FI20001584A/fi
Application granted granted Critical
Publication of FI111014B publication Critical patent/FI111014B/fi

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1273—Polymersomes; Liposomes with polymerisable or polymerised bilayer-forming substances

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
FI20001584A 2000-03-07 2000-07-03 Kationisten lipidien ja kationisten polymeerien synergiaan perustuva geenitransfektion menetelmä FI111014B (fi)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FI20001584A FI111014B (fi) 2000-07-03 2000-07-03 Kationisten lipidien ja kationisten polymeerien synergiaan perustuva geenitransfektion menetelmä
US09/987,156 US20020031502A1 (en) 2000-03-07 2001-11-13 Method for gene transfection using synergistic combinations of cationic lipids and cationic polymers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20001584A FI111014B (fi) 2000-07-03 2000-07-03 Kationisten lipidien ja kationisten polymeerien synergiaan perustuva geenitransfektion menetelmä
FI20001584 2000-07-03

Publications (3)

Publication Number Publication Date
FI20001584A0 FI20001584A0 (fi) 2000-07-03
FI20001584A true FI20001584A (fi) 2002-01-04
FI111014B FI111014B (fi) 2003-05-15

Family

ID=8558710

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20001584A FI111014B (fi) 2000-03-07 2000-07-03 Kationisten lipidien ja kationisten polymeerien synergiaan perustuva geenitransfektion menetelmä

Country Status (2)

Country Link
US (1) US20020031502A1 (fi)
FI (1) FI111014B (fi)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7262056B2 (en) * 2001-11-08 2007-08-28 Mirus Bio Corporation Enhancing intermolecular integration of nucleic acids using integrator complexes
WO2004058308A1 (en) * 2002-12-23 2004-07-15 Board Of Regents The University Of Texas System An efficient non-viral gene/drug delivery system
FR2870741B1 (fr) 2004-05-25 2008-03-14 Coletica Sa Phase lamellaires hydratees ou liposomes, contenant une monoamine grasse ou un polymere cationique favorisant la penetration intercellulaire, et composition cosmetique ou pharmaceutique la contenant.
US20120021042A1 (en) * 2005-09-15 2012-01-26 Steffen Panzner Efficient Method For Loading Amphoteric Liposomes With Nucleic Acid Active Substances

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5627159A (en) * 1994-10-27 1997-05-06 Life Technologies, Inc. Enhancement of lipid cationic transfections in the presence of serum
US6524613B1 (en) * 1997-04-30 2003-02-25 Regents Of The University Of Minnesota Hepatocellular chimeraplasty

Also Published As

Publication number Publication date
US20020031502A1 (en) 2002-03-14
FI20001584A0 (fi) 2000-07-03
FI111014B (fi) 2003-05-15

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