FI20001152A - A method for transforming alkalophilic Bacillus strains - Google Patents

A method for transforming alkalophilic Bacillus strains Download PDF

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Publication number
FI20001152A
FI20001152A FI20001152A FI20001152A FI20001152A FI 20001152 A FI20001152 A FI 20001152A FI 20001152 A FI20001152 A FI 20001152A FI 20001152 A FI20001152 A FI 20001152A FI 20001152 A FI20001152 A FI 20001152A
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Finland
Prior art keywords
bacillus
protoplasts
dna
bacillus cells
dna sequence
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FI20001152A
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Finnish (fi)
Swedish (sv)
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FI119028B (en
Inventor
Eekelen A G Christiaan Van
Der Laan C Johannes Van
J S M Leonardus Mulleners
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Genencor Int
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Publication of FI20001152A publication Critical patent/FI20001152A/en
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Publication of FI119028B publication Critical patent/FI119028B/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A novel expression cassette comprises, in the direction of transcription, a transcriptional regulatory region and a translational initiation region functional in a Bacillus host cell, a DNA sequence encoding a high alkaline proteolytic enzyme and translational and transcriptional termination regions functional in the host cell, where expression of the DNA sequence is under regulatory control of the initiation and termination regions, and at least one of a marker gene and a temp.-sensitive origin of replication of a bacterial plasmid. Also claimed is a method for transforming alkalophilic Bacillus cells which comprises: (a) pretreating the Bacillus cells with lysozyme in an alkaline medium at 20-37 deg.C to form protoplasts, (b) sepg. the protoplasts from the lysozyme, (c) combining the protoplasts with a DNA construct in the presence of a fusogen at 20-37 deg.C, (d) diluting the fusogen and regenerating the Bacillus cells and (e) growing the regenerated Bacillus cells under selective conditions. Also claimed is a DNA sequence encoding a serine protease originating from Bacillus novo species PB92. Also claimed is an isolated DNA or RNA oligonucleotide comprising at least 15 sequential nucleotides capable of hybridising with a sequence that encodes an amino acid sequence of a high alkaline serine protease obtainable from Bacillus novo species PB92.
FI20001152A 1987-02-27 2000-05-15 Method for transforming alkalophilic Bacillus strains FI119028B (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP87200358 1987-02-27
EP87200358 1987-02-27
PCT/NL1988/000007 WO1988006624A2 (en) 1987-02-27 1988-02-26 Molecular cloning and expression of genes encoding proteolytic enzymes
NL8800007 1988-02-26

Publications (2)

Publication Number Publication Date
FI20001152A true FI20001152A (en) 2000-05-15
FI119028B FI119028B (en) 2008-06-30

Family

ID=8197584

Family Applications (3)

Application Number Title Priority Date Filing Date
FI884904A FI105483B (en) 1987-02-27 1988-10-24 Process for increasing the production of an interesting endogenous enzyme in an alkalophilic Bacillus host cell
FI925413A FI106726B (en) 1987-02-27 1992-11-27 Transformation of genes encoding highly alkaline proteases into Bacillus bacteria, and vectors, DNA sequences and probes used in the process
FI20001152A FI119028B (en) 1987-02-27 2000-05-15 Method for transforming alkalophilic Bacillus strains

Family Applications Before (2)

Application Number Title Priority Date Filing Date
FI884904A FI105483B (en) 1987-02-27 1988-10-24 Process for increasing the production of an interesting endogenous enzyme in an alkalophilic Bacillus host cell
FI925413A FI106726B (en) 1987-02-27 1992-11-27 Transformation of genes encoding highly alkaline proteases into Bacillus bacteria, and vectors, DNA sequences and probes used in the process

Country Status (19)

Country Link
US (1) US5217878A (en)
JP (1) JP3026567B2 (en)
KR (1) KR0127560B1 (en)
CN (1) CN88101681A (en)
AT (2) ATE180016T1 (en)
AU (1) AU620026B2 (en)
BG (1) BG49049A3 (en)
BR (1) BR8805647A (en)
CA (1) CA1339100C (en)
DE (2) DE3856339T2 (en)
DK (2) DK175738B1 (en)
FI (3) FI105483B (en)
HU (1) HU213220B (en)
LT (1) LT4000B (en)
LV (1) LV10307B (en)
NO (1) NO884423L (en)
PT (1) PT86864B (en)
RU (1) RU2023723C1 (en)
WO (1) WO1988006624A2 (en)

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Also Published As

Publication number Publication date
DK200401262A (en) 2004-08-20
NO884423D0 (en) 1988-10-05
FI884904A0 (en) 1988-10-24
KR890700665A (en) 1989-04-26
JP3026567B2 (en) 2000-03-27
DK593988A (en) 1988-10-26
DK593988D0 (en) 1988-10-26
BR8805647A (en) 1989-10-31
DE3856331D1 (en) 1999-06-17
DE3856339D1 (en) 1999-07-15
DE3856331T2 (en) 1999-10-14
LT4000B (en) 1996-06-25
CN88101681A (en) 1988-11-09
CA1339100C (en) 1997-07-29
LTIP1820A (en) 1995-08-25
LV10307B (en) 1995-08-20
AU620026B2 (en) 1992-02-13
HU213220B (en) 1997-03-28
DE3856339T2 (en) 1999-11-04
DK175852B1 (en) 2005-04-04
FI105483B (en) 2000-08-31
NO884423L (en) 1988-10-05
FI106726B (en) 2001-03-30
ATE180016T1 (en) 1999-05-15
AU1398088A (en) 1988-09-26
WO1988006624A2 (en) 1988-09-07
DK175738B1 (en) 2005-02-07
PT86864A (en) 1988-03-01
RU2023723C1 (en) 1994-11-30
JPH01502876A (en) 1989-10-05
FI925413A (en) 1992-11-27
FI884904A (en) 1988-10-24
FI925413A0 (en) 1992-11-27
PT86864B (en) 1992-05-29
FI119028B (en) 2008-06-30
BG49049A3 (en) 1991-07-15
LV10307A (en) 1994-10-20
KR0127560B1 (en) 1997-12-29
WO1988006624A3 (en) 1988-11-17
US5217878A (en) 1993-06-08
ATE181110T1 (en) 1999-06-15

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