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Antibioticos SA
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Antibioticos SA
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Application filed by Antibioticos SAfiledCriticalAntibioticos SA
Priority to ES534757ApriorityCriticalpatent/ES8602933A1/en
Publication of ES534757A0publicationCriticalpatent/ES534757A0/en
Publication of ES8602933A1publicationCriticalpatent/ES8602933A1/en
The process consists of (a) selecting micro-organisms which produce penicillinacylase and isolating from them the DNA fragments contg. the genetic information for prodn. of penicillinacylase, and inserting H in a DNA segment to obtain the corresp. recombinant segments (b) introducing these segments into the cells of a host micro-organism, not a penicillinacylase producer, of auxotrophic strains for cpds. able to form amide bonds with phenyl acetic acid and (c) isolating the modifications with penicillinacylase activity and cultivating the modified cells in a suitable culture medium.
ES534757A1984-07-311984-07-31Gene cloning by recombinant DNA technique
ExpiredES8602933A1
(en)
Polynucleotide sequences, polypeptides coded by them and exhibiting activity resulting in exposrting branched-chain amino acids, host cell an method of producing branched-chain amino acids
Bacillus subtilis strain whose extracellular protease activities are reduced, method for obtaining the strain and method for secreting proteins by using the strain