ES8801810A1 - Un procedimiento para la preparacion de sustratos derivados de xantilio. - Google Patents
Un procedimiento para la preparacion de sustratos derivados de xantilio.Info
- Publication number
- ES8801810A1 ES8801810A1 ES555370A ES555370A ES8801810A1 ES 8801810 A1 ES8801810 A1 ES 8801810A1 ES 555370 A ES555370 A ES 555370A ES 555370 A ES555370 A ES 555370A ES 8801810 A1 ES8801810 A1 ES 8801810A1
- Authority
- ES
- Spain
- Prior art keywords
- substrates
- water soluble
- enzymes
- trypsin
- derivative substrates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/08—Tripeptides
- C07K5/0821—Tripeptides with the first amino acid being heterocyclic, e.g. His, Pro, Trp
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/08—Tripeptides
- C07K5/0802—Tripeptides with the first amino acid being neutral
- C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
- C07K5/0806—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/40—Rhodamine derivatives
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/81—Protease inhibitors
- G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
- G01N2333/8121—Serpins
- G01N2333/8128—Antithrombin III
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, e.g. Konjac gum, Locust bean gum, Guar gum
- G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/802—Chromogenic or luminescent peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/28—Bound to a nonpeptide drug, nonpeptide label, nonpeptide carrier, or a nonpeptide resin
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Neurosurgery (AREA)
- Hematology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PROCEDIMIENTO PARA LA PREPARACION DE SUSTRATOS DERIVADOS DE XANTILIO, DE FORMULAS GENERALES (I) Y (II). CONSISTE EN PREPARARLOS A PARTIR DE RODAMINA 110 Y RODOL, DE FORMULAS GENERALES (III). DICHOS SUSTRATOS PERMITEN LAS MEDIDAS ESPECTROFOTOMETRICAS POR FLUORESCENCIA DE ENZIMAS SIMILARES A LA TRIPSINA, SIN LA ADICION DE ADITIVOS DISOLVENTES ORGANICOS O AGENTES ESPECIALES DE SOLUBILIZACION EN AGUA. ESTOS SUSTRATOS PRESENTAN UNA SENSIBILIDAD INCREMENTADA PARA DETERMINAR BAJOS NIVELES DE ACTIVIDAD DE ENZIMAS COMO LA TRIPSINA, TALES COMO ENZIMAS PROTEOLITICAS, COFACTORES, ACTIVADORES E INHIBIDORES.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/737,761 US4694070A (en) | 1985-05-28 | 1985-05-28 | Water soluble xanthylium derivatives substrates |
Publications (2)
Publication Number | Publication Date |
---|---|
ES555370A0 ES555370A0 (es) | 1988-02-16 |
ES8801810A1 true ES8801810A1 (es) | 1988-02-16 |
Family
ID=24965213
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES555370A Expired ES8801810A1 (es) | 1985-05-28 | 1986-05-27 | Un procedimiento para la preparacion de sustratos derivados de xantilio. |
Country Status (9)
Country | Link |
---|---|
US (1) | US4694070A (es) |
EP (1) | EP0233197B1 (es) |
JP (1) | JPH0759592B2 (es) |
CN (1) | CN1027589C (es) |
AU (1) | AU5950486A (es) |
CA (1) | CA1276391C (es) |
DE (1) | DE3689171T2 (es) |
ES (1) | ES8801810A1 (es) |
WO (1) | WO1986007061A1 (es) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8721302D0 (en) * | 1987-09-10 | 1987-10-14 | London King S College | Substrates for assay of enzymes |
WO1995019359A1 (en) * | 1994-01-12 | 1995-07-20 | Massachusetts Institute Of Technology | Process for making xanthene or cubane based compounds, and protease inhibitors |
US8092784B2 (en) | 2005-11-30 | 2012-01-10 | Biotium, Inc. | Enzyme substrate comprising a functional dye and associated technology and methods |
FR2978163B1 (fr) * | 2011-07-22 | 2013-08-30 | Stago Diagnostica | Utilisation d'un substrat dans une methode pour mesurer l'activite des enzymes proteolytiques disponibles |
FR2978164B1 (fr) * | 2011-07-22 | 2013-08-30 | Stago Diagnostica | Utilisation d'un substrat dans une methode pour mesurer l'activite des enzymes proteolytiques |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE7801373L (sv) * | 1978-02-07 | 1979-08-08 | Kabi Ab | Lett spjelkbara substrat for kvantifiering av proteaser |
US4275153A (en) * | 1978-08-03 | 1981-06-23 | American Hospital Supply Corporation | Analytical fluorogenic substrates for proteolytic enzymes |
US4336186A (en) * | 1978-08-03 | 1982-06-22 | Gargiulo Robert J | Analytical fluorogenic substrates for proteolytic enzymes |
US4294923A (en) * | 1979-04-23 | 1981-10-13 | Smith Robert E | Substrates and method for determining enzymes |
US4557862A (en) * | 1983-10-28 | 1985-12-10 | University Patents, Inc. | Rhodamine derivatives as fluorogenic substrates for proteinases |
-
1985
- 1985-05-28 US US06/737,761 patent/US4694070A/en not_active Expired - Lifetime
-
1986
- 1986-05-01 CA CA000508080A patent/CA1276391C/en not_active Expired - Fee Related
- 1986-05-26 CN CN86103598.4A patent/CN1027589C/zh not_active Expired - Fee Related
- 1986-05-27 WO PCT/US1986/001136 patent/WO1986007061A1/en active IP Right Grant
- 1986-05-27 ES ES555370A patent/ES8801810A1/es not_active Expired
- 1986-05-27 AU AU59504/86A patent/AU5950486A/en not_active Abandoned
- 1986-05-27 EP EP86903893A patent/EP0233197B1/en not_active Expired - Lifetime
- 1986-05-27 DE DE3689171T patent/DE3689171T2/de not_active Expired - Fee Related
- 1986-05-27 JP JP61503075A patent/JPH0759592B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
ES555370A0 (es) | 1988-02-16 |
CN1027589C (zh) | 1995-02-08 |
AU5950486A (en) | 1986-12-24 |
EP0233197A1 (en) | 1987-08-26 |
WO1986007061A1 (en) | 1986-12-04 |
EP0233197B1 (en) | 1993-10-13 |
CN86103598A (zh) | 1987-02-25 |
CA1276391C (en) | 1990-11-13 |
US4694070A (en) | 1987-09-15 |
JPH0759592B2 (ja) | 1995-06-28 |
DE3689171T2 (de) | 1994-05-19 |
EP0233197A4 (en) | 1989-02-16 |
DE3689171D1 (de) | 1993-11-18 |
JPS62503035A (ja) | 1987-12-03 |
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