ES8609722A1 - Procedimiento para el analisis de la hemoglobina - Google Patents

Procedimiento para el analisis de la hemoglobina

Info

Publication number
ES8609722A1
ES8609722A1 ES549320A ES549320A ES8609722A1 ES 8609722 A1 ES8609722 A1 ES 8609722A1 ES 549320 A ES549320 A ES 549320A ES 549320 A ES549320 A ES 549320A ES 8609722 A1 ES8609722 A1 ES 8609722A1
Authority
ES
Spain
Prior art keywords
hba2
haemoglobin
analysis
hemolysate
obtainment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES549320A
Other languages
English (en)
Other versions
ES549320A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CHEMICAL LAB Srl
Chemical Laboratories Srl
Original Assignee
CHEMICAL LAB Srl
Chemical Laboratories Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CHEMICAL LAB Srl, Chemical Laboratories Srl filed Critical CHEMICAL LAB Srl
Publication of ES549320A0 publication Critical patent/ES549320A0/es
Publication of ES8609722A1 publication Critical patent/ES8609722A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODO DE ANALISIS DE HEMOGLOBULINA DE HBA2. COMPRENDE MEZCLAR UN HEMOLIZADO DE SANGRE Y UNA RESINA ANIONICA, TAL COMO RESINA DEAE 52, EN CONCENTRACION DE 50 A 400 G/L CON ADICION DE UNA DISOLUCION ACUOSADE GLICINA QUE TIENE UNA CONCENTRACION COMPRENDIDA ENTRE 0,05 Y 0,3 MOLES/LITRO Y SE HALLA TAMPONADA A UN PH ENTRE 7,1 Y 7,8; AGITAR LA MEZCLA CON UN AGITADOR GIRATORIO DURANTE UNOS CINCO MINUTOS PARA CONSEGUIR LA SEPARACION DE LA MEZCLA EN DOS FASES; SEPARACION DE LA FASE SOBRENADANTE MEDIANTE FILTRACION A TRAVES DE UN FILTRO DE PISTON; Y ANALISIS FOTOMETRICO DE ESTE FILTRADO POR MEDIDA DE LA ABSORBENCIA A 400-425 NM. EL METODO TIENE APLICACIONES EN LA PRACTICA QUIMICO-CLINICA.
ES549320A 1984-11-28 1985-11-27 Procedimiento para el analisis de la hemoglobina Expired ES8609722A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT23779/84A IT1177354B (it) 1984-11-28 1984-11-28 Procedimento per l'analisi dell'emoglobina hba2

Publications (2)

Publication Number Publication Date
ES549320A0 ES549320A0 (es) 1986-09-01
ES8609722A1 true ES8609722A1 (es) 1986-09-01

Family

ID=11209909

Family Applications (1)

Application Number Title Priority Date Filing Date
ES549320A Expired ES8609722A1 (es) 1984-11-28 1985-11-27 Procedimiento para el analisis de la hemoglobina

Country Status (3)

Country Link
EP (1) EP0183181A3 (es)
ES (1) ES8609722A1 (es)
IT (1) IT1177354B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4810391A (en) * 1987-11-06 1989-03-07 Bio-Rad Laboratories, Inc. Separation of hemoglobin A2 from hemoglobin mixture
US4980058A (en) * 1987-11-06 1990-12-25 Bio-Rad Laboratories, Inc. Separation of hemoglobin A2 from hemoglobin mixture

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2024829B (en) * 1978-06-28 1982-08-04 Amicon Corp Method and product for separation of glycoproteins
JPS55101050A (en) * 1979-01-26 1980-08-01 Sogo Seibutsu Igaku Kenkyusho:Kk Simple dispensation measuring method for hemoglobin glucoside in blood
US4209373A (en) * 1979-06-01 1980-06-24 Corning Glass Works Acidic agar gel electrochromatography of hemoglobins
US4407961A (en) * 1981-03-16 1983-10-04 Sanders James L Ion-exchange system and method for isolation and determination of glycosylated hemoglobin in human blood
US4436820A (en) * 1982-02-01 1984-03-13 Reiter Paul C Method and apparatus for glycosylated hemoglobin separating and measuring fractions
US4409335A (en) * 1982-05-28 1983-10-11 Bio-Rad Laboratories, Inc. Method for eliminating glucose dependent Schiff base effect from hemoglobin A1 assay

Also Published As

Publication number Publication date
EP0183181A3 (en) 1986-09-03
IT1177354B (it) 1987-08-26
IT8423779A1 (it) 1986-05-28
IT8423779A0 (it) 1984-11-28
EP0183181A2 (en) 1986-06-04
ES549320A0 (es) 1986-09-01

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