ES8608578A1 - Procedimiento para la sintesis de una proteina deseada se- leccionada del grupo de amilasa - Google Patents

Procedimiento para la sintesis de una proteina deseada se- leccionada del grupo de amilasa

Info

Publication number
ES8608578A1
ES8608578A1 ES545148A ES545148A ES8608578A1 ES 8608578 A1 ES8608578 A1 ES 8608578A1 ES 545148 A ES545148 A ES 545148A ES 545148 A ES545148 A ES 545148A ES 8608578 A1 ES8608578 A1 ES 8608578A1
Authority
ES
Spain
Prior art keywords
hydrolases
carbonyl
subtilisin
prokaryotic
kcat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES545148A
Other languages
English (en)
Other versions
ES545148A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/614,612 external-priority patent/US4760025A/en
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of ES8608578A1 publication Critical patent/ES8608578A1/es
Publication of ES545148A0 publication Critical patent/ES545148A0/es
Expired legal-status Critical Current

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE PROTEINAS DEL GRUPO DE AMILASA, DE MUTANTES DE SUBTILISINA Y PROTEINAS ECUARIOTICAS, MEDIANTE TECNICAS RECOMBINANTES EN HOSPEDANTES APROPIADOS. COMPRENDE LA PREPARACION DE UN MEDIO NUTRIENTE DE CULTIVO PARA EL DESARROLLO DE BACILLUS NORMALMENTE ESPORULANTE, TRANSFORMADO CON AL MENOS UN RESTO DE ADN QUE CODIFICA UN POLIPEPTIDO QUE DE OTRO MODO NO SERIA EXPRESADO POR EL BACILLUS, DE MODO QUE EL BACILLUS NO SEGREGA SUBTILISINA O PROTEASA NEUTRA; INOCULACION DEL MEDIO DE CULTIVO CON DICHO BACILLUS; CULTIVAR ESTE EN CONDICIONES ADECUADAS PARA SU DESARROLLO HASTA QUE SE HALLA ACUMULADA LA PROTEINA AMILASA; Y RECUPERAR ESTA DEL MEDIO DE CULTIVO. ESTAS PROTEINAS TIENEN APLICACIONES EN DETERGENTES PARA ELIMINACION DE MANCHAS Y EN LA ELABORACION Y TRATAMIENTO DE ALIMENTOS.
ES545148A 1983-06-24 1985-07-12 Procedimiento para la sintesis de una proteina deseada se- leccionada del grupo de amilasa Expired ES8608578A1 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US50741983A 1983-06-24 1983-06-24
US61461784A 1984-05-29 1984-05-29
US61461584A 1984-05-29 1984-05-29
US06/614,612 US4760025A (en) 1984-05-29 1984-05-29 Modified enzymes and methods for making same

Publications (2)

Publication Number Publication Date
ES8608578A1 true ES8608578A1 (es) 1986-07-16
ES545148A0 ES545148A0 (es) 1986-07-16

Family

ID=27504464

Family Applications (3)

Application Number Title Priority Date Filing Date
ES533645A Expired ES8604646A1 (es) 1983-06-24 1984-06-22 Metodo para preparar una carbonil-hidrolasa procariotica
ES545148A Expired ES8608578A1 (es) 1983-06-24 1985-07-12 Procedimiento para la sintesis de una proteina deseada se- leccionada del grupo de amilasa
ES545147A Expired ES8701839A1 (es) 1983-06-24 1985-07-12 Metodo para la obtencion de una pluralidad de radicales de adn mutantes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES533645A Expired ES8604646A1 (es) 1983-06-24 1984-06-22 Metodo para preparar una carbonil-hidrolasa procariotica

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES545147A Expired ES8701839A1 (es) 1983-06-24 1985-07-12 Metodo para la obtencion de una pluralidad de radicales de adn mutantes

Country Status (1)

Country Link
ES (3) ES8604646A1 (es)

Also Published As

Publication number Publication date
ES8701839A1 (es) 1986-12-16
ES533645A0 (es) 1986-02-16
ES545147A0 (es) 1986-12-16
ES545148A0 (es) 1986-07-16
ES8604646A1 (es) 1986-02-16

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