ES8607390A1 - Nonradioactive probe and process for the characterisation of a determined nucleotide sequence and means for the production of this nonradioactive probe - Google Patents

Nonradioactive probe and process for the characterisation of a determined nucleotide sequence and means for the production of this nonradioactive probe

Info

Publication number
ES8607390A1
ES8607390A1 ES539964A ES539964A ES8607390A1 ES 8607390 A1 ES8607390 A1 ES 8607390A1 ES 539964 A ES539964 A ES 539964A ES 539964 A ES539964 A ES 539964A ES 8607390 A1 ES8607390 A1 ES 8607390A1
Authority
ES
Spain
Prior art keywords
nonradioactive probe
nonradioactive
probe
characterisation
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES539964A
Other languages
Spanish (es)
Other versions
ES539964A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut Pasteur de Lille
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut Pasteur de Lille filed Critical Centre National de la Recherche Scientifique CNRS
Publication of ES539964A0 publication Critical patent/ES539964A0/en
Publication of ES8607390A1 publication Critical patent/ES8607390A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Process for obtaining a vector DNA in which at least one of the bases is at least partly substituted by a modified base capable of being biologically incorporated into the genome DNA of a microorganism, this process concerning the bringing about and maintaining of the culture of this microorganism previously transformed by the corresponding vector DNA, in which the corresponding natural base is not modified, with a culture medium containing the modified base as replacement for the corresponding natural base. This process is carried out under protection from radiation of wavelengths longer than 400 nanometres.
ES539964A 1984-01-31 1985-01-30 Nonradioactive probe and process for the characterisation of a determined nucleotide sequence and means for the production of this nonradioactive probe Expired ES8607390A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8401480A FR2567147B2 (en) 1984-01-31 1984-01-31 NON-RADIOACTIVE PROBE AND METHOD FOR CHARACTERIZING A DETERMINED NUCLEOTIDE SEQUENCE AND MEANS FOR THE PRODUCTION OF THIS NON-RADIOACTIVE PROBE

Publications (2)

Publication Number Publication Date
ES539964A0 ES539964A0 (en) 1986-06-01
ES8607390A1 true ES8607390A1 (en) 1986-06-01

Family

ID=9300631

Family Applications (1)

Application Number Title Priority Date Filing Date
ES539964A Expired ES8607390A1 (en) 1984-01-31 1985-01-30 Nonradioactive probe and process for the characterisation of a determined nucleotide sequence and means for the production of this nonradioactive probe

Country Status (2)

Country Link
ES (1) ES8607390A1 (en)
FR (1) FR2567147B2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2230753A1 (en) * 1995-08-30 1997-03-06 New York Medical College Methods for labeling dna ends with halogenated nucleotides and detecting same with antibodies

Also Published As

Publication number Publication date
ES539964A0 (en) 1986-06-01
FR2567147B2 (en) 1987-01-30
FR2567147A2 (en) 1986-01-10

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