ES8602123A1 - Un metodo para clonar un gen que codifica la cobre-meta-lotioneina de levadura - Google Patents

Un metodo para clonar un gen que codifica la cobre-meta-lotioneina de levadura

Info

Publication number
ES8602123A1
ES8602123A1 ES84534931A ES534931A ES8602123A1 ES 8602123 A1 ES8602123 A1 ES 8602123A1 ES 84534931 A ES84534931 A ES 84534931A ES 534931 A ES534931 A ES 534931A ES 8602123 A1 ES8602123 A1 ES 8602123A1
Authority
ES
Spain
Prior art keywords
copper
metallothionein gene
yeast
yeast copper
metallothionein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES84534931A
Other languages
English (en)
Other versions
ES534931A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp
Publication of ES534931A0 publication Critical patent/ES534931A0/es
Publication of ES8602123A1 publication Critical patent/ES8602123A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/825—Metallothioneins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

METODO PARA LA CLONACION DE TODA O UN PARTE DE LA UNIDAD DE EXPRESION DEL GEN DE COBRE-METALOTIONEINA DE LEVADURA. COMPRENDE TRATAR ADN CROMOSOMICO PROCEDENTE DE UNA CELULA DE LEVADURA, QUE PRODUCE COBRE-METALOTIONEINA, CON UNA O MAS ENDONUCLEASAS DE RESTRICCION; LIGAR EL FRAGMENTO DE ADN ESCINDIDO A UN VECTOR DE CLONACION DE ADN PARA INSERTARLE EL FRAGMENTO DE ADN ESCINDIDO; TRANSFORMAR UNA CELULA DE LEVADURA HOSPEDANTE CON EL VECTOR QUE TIENE INSERTADO EL CITADO FRAGMENTO DE ADN; Y SELECCIONAR LOS TRANSFORMANTES QUE LLEVAN TODA O PARTE DE LA UNIDAD DE EXPRESION DE DICHO GEN. LAS METALOTIONEINAS TIENEN APLICACIONES PARA QUELAR METALES DE TRANSICION EN CORRIENTES RESIDUALES INDUSTRIALES, Y PARA EL TRATRAMIENTO DE ENVENENAMIENTO POR METALES.
ES84534931A 1983-08-08 1984-08-07 Un metodo para clonar un gen que codifica la cobre-meta-lotioneina de levadura Expired ES8602123A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52066883A 1983-08-08 1983-08-08
US58465784A 1984-03-02 1984-03-02

Publications (2)

Publication Number Publication Date
ES534931A0 ES534931A0 (es) 1985-11-01
ES8602123A1 true ES8602123A1 (es) 1985-11-01

Family

ID=27060223

Family Applications (1)

Application Number Title Priority Date Filing Date
ES84534931A Expired ES8602123A1 (es) 1983-08-08 1984-08-07 Un metodo para clonar un gen que codifica la cobre-meta-lotioneina de levadura

Country Status (6)

Country Link
EP (1) EP0134773A1 (es)
AU (1) AU3163784A (es)
DK (1) DK381684A (es)
ES (1) ES8602123A1 (es)
GR (1) GR80044B (es)
PT (1) PT79046B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4940661A (en) * 1984-05-17 1990-07-10 Genentech, Inc. Metallothionein transcription control sequences and use thereof
US4935350A (en) * 1985-11-18 1990-06-19 Amgen Materials and methods for controlling plasmid copy number and stability
AU614959B2 (en) * 1987-05-07 1991-09-19 Commonwealth Scientific And Industrial Research Organisation Expression system
TW282490B (es) * 1992-12-15 1996-08-01 Ciba Geigy Ag
AU3920293A (en) * 1993-03-16 1994-10-11 Board Of Regents Of The University Of Nebraska, The Novel metal binding agents, and methods and compositions for their use to treat metal toxicity
CN1062306C (zh) * 1996-02-06 2001-02-21 北京大学 酵母菌Cu-金属硫蛋白及其生产方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0078154A3 (en) * 1981-10-28 1985-01-09 The Regents Of The University Of California Plasmids and cells for heterologous expression of metallothioneins and processes for producing and using the metallothionein

Also Published As

Publication number Publication date
EP0134773A1 (en) 1985-03-20
AU3163784A (en) 1985-02-14
ES534931A0 (es) 1985-11-01
DK381684A (da) 1985-02-09
PT79046A (en) 1984-09-01
PT79046B (en) 1986-11-13
DK381684D0 (da) 1984-08-08
GR80044B (en) 1984-12-10

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