ES8600782A1 - Extn. of refractile material from host cells - Google Patents

Extn. of refractile material from host cells

Info

Publication number
ES8600782A1
ES8600782A1 ES528321A ES528321A ES8600782A1 ES 8600782 A1 ES8600782 A1 ES 8600782A1 ES 528321 A ES528321 A ES 528321A ES 528321 A ES528321 A ES 528321A ES 8600782 A1 ES8600782 A1 ES 8600782A1
Authority
ES
Spain
Prior art keywords
host cell
protein
refractile
extn
host cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES528321A
Other languages
Spanish (es)
Other versions
ES528321A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of ES8600782A1 publication Critical patent/ES8600782A1/en
Publication of ES528321A0 publication Critical patent/ES528321A0/en
Granted legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Treatment of heterologous expression prod. (I) from a host cell culture comprises contact of a refractile material contg. (I) with a denaturing soln.. Extrn. of refractile material (II) including heterologous protein, from host cell material comprises (a) suspending the material in buffer (b) disrupting the material to release (II) and (c) performing low speed centrifugation to form a pellet of (II). Reactivation of a protein formed as (I) in a host cell comprises treatment of a weakly denaturing soln. of the protein with a sulphydryl cpd. in presence of its corresp. disulphide.
ES528321A 1982-12-22 1983-12-22 A METHOD FOR TREATING A REFRACTILE PROTEIN PRODUCED AS A HETEROLOGICAL EXPRESSION PRODUCT IN A CELL HOSPEDANT CULTURE. Granted ES528321A0 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US45225282A 1982-12-22 1982-12-22
US45218782A 1982-12-22 1982-12-22
US45236382A 1982-12-22 1982-12-22
US45234482A 1982-12-22 1982-12-22

Publications (2)

Publication Number Publication Date
ES8600782A1 true ES8600782A1 (en) 1985-11-01
ES528321A0 ES528321A0 (en) 1985-11-01

Family

ID=27503997

Family Applications (1)

Application Number Title Priority Date Filing Date
ES528321A Granted ES528321A0 (en) 1982-12-22 1983-12-22 A METHOD FOR TREATING A REFRACTILE PROTEIN PRODUCED AS A HETEROLOGICAL EXPRESSION PRODUCT IN A CELL HOSPEDANT CULTURE.

Country Status (1)

Country Link
ES (1) ES528321A0 (en)

Also Published As

Publication number Publication date
ES528321A0 (en) 1985-11-01

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20050702