ES8501531A1 - Un metodo y a unos reactivos para determinar ligandos en los fluidos biologicos - Google Patents

Un metodo y a unos reactivos para determinar ligandos en los fluidos biologicos

Info

Publication number
ES8501531A1
ES8501531A1 ES527082A ES527082A ES8501531A1 ES 8501531 A1 ES8501531 A1 ES 8501531A1 ES 527082 A ES527082 A ES 527082A ES 527082 A ES527082 A ES 527082A ES 8501531 A1 ES8501531 A1 ES 8501531A1
Authority
ES
Spain
Prior art keywords
aminomethylfluorescein
derivatives
fluid
aminomethylfluorescein derivatives
urine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES527082A
Other languages
English (en)
Other versions
ES527082A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of ES527082A0 publication Critical patent/ES527082A0/es
Publication of ES8501531A1 publication Critical patent/ES8501531A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
    • C07D493/10—Spiro-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J41/00—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring
    • C07J41/0005—Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring the nitrogen atom being directly linked to the cyclopenta(a)hydro phenanthrene skeleton
    • C07J41/0016—Oximes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones

Abstract

METODO DE INMUNOENSAYO PARA DETERMINAR LIGANDOS EN LOS FLUIDOS BIOLOGICOS.CONSISTE EN TRATAR LA MUESTRA QUE CONTIENE EL LIGANDO A DETERMINAR CON UN MARCADOR DE FORMULA (I) Y CON UN ANTICUERPO CAPAZ DE RECONOCER ESPECIFICAMENTE EL LIGANDO Y EL MARCADOR DE MODO QUE EL LIGANDO Y EL MARCADOR COMPITAN POR LO SITIOS LIMITANTES DEL ANTICUERPO RESULTANDO LA FORMACION DE COMPLEJOS LIGANDO-ANTICUERPO Y MARCADOR-ANTICUERPO, Y A CONTINUACION EXCITAR LA MEZCLA CON LUZ FLUORESCENTE Y MEDIR LA POLARIZACION DE LA FLUORESCENCIA EMITIDA POR EL MARCADOR UNIDO AL ANTICUERPO COMO MEDIDA DE LA CANTIDAD DE LIGANDO EN LA MUESTRA. DONDE R4 ES UN GRUPO -CH2-NH-RL, RL ES UN ANALOGO DE LIGANDO; R2 ES H O R1 Y R3 ES H, AMINO O CARBOXILO.
ES527082A 1982-11-08 1983-11-07 Un metodo y a unos reactivos para determinar ligandos en los fluidos biologicos Expired ES8501531A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/440,043 US4510251A (en) 1982-11-08 1982-11-08 Fluorescent polarization assay for ligands using aminomethylfluorescein derivatives as tracers

Publications (2)

Publication Number Publication Date
ES527082A0 ES527082A0 (es) 1984-11-16
ES8501531A1 true ES8501531A1 (es) 1984-11-16

Family

ID=23747184

Family Applications (1)

Application Number Title Priority Date Filing Date
ES527082A Expired ES8501531A1 (es) 1982-11-08 1983-11-07 Un metodo y a unos reactivos para determinar ligandos en los fluidos biologicos

Country Status (5)

Country Link
US (1) US4510251A (es)
EP (1) EP0110186A1 (es)
JP (2) JPH0786110B2 (es)
CA (1) CA1217478A (es)
ES (1) ES8501531A1 (es)

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US4665020A (en) * 1984-05-30 1987-05-12 United States Department Of Energy Flow cytometer measurement of binding assays
EP0200960A1 (en) * 1985-05-08 1986-11-12 Abbott Laboratories Total estriol fluorescence polarization immunoassay
US4855226A (en) * 1985-06-07 1989-08-08 Beckman Instruments, Inc. Novel competitive assay for theophylline and reagent for use therein
EP0218010A3 (en) * 1985-07-10 1988-01-07 Abbott Laboratories Ligand detection method and substituted carboxyfluorescein tracers therefor
EP0232736A1 (en) * 1986-02-06 1987-08-19 Abbott Laboratories Preparation of 4'-aminomethylfluorescein derivatives for use in fluorescence polarization immunoassays
US5336622A (en) * 1986-04-25 1994-08-09 Abbott Laboratories Tracers for use in flecainide fluorescence polarization immunoassay
US5202270A (en) * 1986-05-15 1993-04-13 Abbott Laboratories Cocaine and cocaine metabolites assay tracers, immunogens and antibodies
US5221629A (en) * 1986-05-21 1993-06-22 Abbott Laboratories Phencyclidine and phencyclidine metabolites assay, tracers, immunogens and antibodies
US5124457A (en) * 1986-05-21 1992-06-23 Abbott Laboratories Phencyclidine and phencyclidine metabolites assay, tracers, immunogens and antibodies
US5155212A (en) * 1986-05-21 1992-10-13 Abbott Laboratories Phencyclidine and phencyclidine metabolites assay, tracers, immunogens, antibodies and reagent kit
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US5145791A (en) * 1986-07-09 1992-09-08 Abbott Laboratories 3-methoxy-4-hydroxyphenylglycol fluorescence polarization immunoassay
US4939264A (en) * 1986-07-14 1990-07-03 Abbott Laboratories Immunoassay for opiate alkaloids and their metabolites; tracers, immunogens and antibodies
US4784961A (en) * 1987-03-23 1988-11-15 Abbott Laboratories Fluorescence polarization method for monitoring fetal lung maturity
US5223627A (en) * 1986-07-15 1993-06-29 Abbott Laboratories Fluorescence polarization method for monitoring fetal lung maturity
US4868132A (en) * 1987-02-03 1989-09-19 Abbott Laboratories Fluorescence polarization immunoassay for amphetamine/methamphetamine
US5427960A (en) * 1987-03-27 1995-06-27 Abbott Laboratories Fluorescence polarization assay for cyclosporin A and metabolites and related immunogens and antibodies
US4948563A (en) * 1987-05-08 1990-08-14 Abbott Laboratories Waste container insert for washing automated immunoassay apparatus probe
US4970074A (en) * 1987-06-29 1990-11-13 Abbott Laboratories Fluorophores for encapsulation into liposomes
US4912208A (en) * 1987-06-29 1990-03-27 Abbott Laboratories Fluorophores for encapsulation into liposomes
US4894348A (en) * 1987-07-01 1990-01-16 Ronald Robert C Fluorescein-conjugated proteins with enhanced fluorescence
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US5045479A (en) * 1988-07-05 1991-09-03 The Johns Hopkins University Continuous flow competitive assay with reference system
US5262333A (en) * 1988-10-28 1993-11-16 Abbott Laboratories Method and reagents for detecting amphetamine and/or D-methamphetamine in biological samples
EP0373508A3 (en) * 1988-12-12 1992-07-08 Abbott Laboratories Barbiturate assay, tracers, immunogens and antibodies
US5073629A (en) * 1989-01-23 1991-12-17 Abbott Laboratories Methadone fluorescence polarization immunoassay
US5248791A (en) * 1989-04-10 1993-09-28 Abbott Laboratories Reagents, methods and kits for an amphetamine-class fluorescence polarization immunoassay
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AU6309690A (en) * 1989-09-28 1991-04-11 Abbott Laboratories Stabilization of monoclonal antibody for use in fluorescent polarization techniques
US5099020A (en) * 1989-11-27 1992-03-24 Abbott Laboratories Barbiturate assay compositions and methods
US5099000A (en) * 1989-12-22 1992-03-24 Abbott Laboratories Derivatives and analogues of monoethylglycinexylidide and their production and use
EP0457213B1 (en) * 1990-05-16 1997-07-23 Abbott Laboratories Barbiturate assay, tracers, immunogens, antibodies and kit
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US5352803A (en) * 1992-03-30 1994-10-04 Abbott Laboratories 5(6)-methyl substituted fluorescein derivatives
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US5491071A (en) * 1993-08-03 1996-02-13 Abbott Laboratories Reagents and methods for the detection and quantification of testosterone in fluid samples
US7083984B2 (en) 1993-09-24 2006-08-01 Biosite, Inc. Hybrid phthalocyanine derivatives and their uses
US5824799A (en) * 1993-09-24 1998-10-20 Biosite Diagnostics Incorporated Hybrid phthalocyanine derivatives and their uses
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Also Published As

Publication number Publication date
JPS59104388A (ja) 1984-06-16
JPH0786110B2 (ja) 1995-09-20
EP0110186A1 (en) 1984-06-13
ES527082A0 (es) 1984-11-16
JPH0741482A (ja) 1995-02-10
CA1217478A (en) 1987-02-03
US4510251A (en) 1985-04-09
JPH0762019B2 (ja) 1995-07-05

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970612