ES8306790A1 - Glucose isomerase immobilisation - Google Patents

Glucose isomerase immobilisation

Info

Publication number
ES8306790A1
ES8306790A1 ES513529A ES513529A ES8306790A1 ES 8306790 A1 ES8306790 A1 ES 8306790A1 ES 513529 A ES513529 A ES 513529A ES 513529 A ES513529 A ES 513529A ES 8306790 A1 ES8306790 A1 ES 8306790A1
Authority
ES
Spain
Prior art keywords
pref
matrix
immobilisation
enzyme
glucose isomerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES513529A
Other languages
Spanish (es)
Other versions
ES513529A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Honeywell UOP LLC
Original Assignee
UOP LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UOP LLC filed Critical UOP LLC
Priority to ES513529A priority Critical patent/ES8306790A1/en
Publication of ES513529A0 publication Critical patent/ES513529A0/en
Publication of ES8306790A1 publication Critical patent/ES8306790A1/en
Expired legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The immobilisation of glucose isomerase is enhanced by first impregnating the matrix with at least 0.1 mmole Mg(2+) per g of matrix. Pref. amt. of Mg(2+) is 0.1-2 mmole/g. Impregnation is pref. by contact with a soln. of (in)organic Mg salt, pref. the halide, sulphate, nitrate, hypophosphite, fluorosilicate, acetate or lactate. The soln. is pref. 1-25 mM in Mg(2+). MgSO4 is the pref. salt. - Pref. the support is an inorganic oxide impregnated with a polyamine which is then crosslinked with excess of a bifunctional reagent to provide pendant functional gps. - The half-life of the immobilised enzyme is increased, and time for the immobilisation may be decreased. The enzyme may be used for converting glucose to fructose. DEAB- DE3224024 C Glucose isomerase is immobilised on a carrier matrix by treating the matrix with at least 0.1 mmoles of divalent Mg ions, pref. 0.1-2m moles MgSO4 per Gram carrier matrix before contact with the enzyme.
ES513529A 1982-06-28 1982-06-28 Glucose isomerase immobilisation Expired ES8306790A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES513529A ES8306790A1 (en) 1982-06-28 1982-06-28 Glucose isomerase immobilisation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES513529A ES8306790A1 (en) 1982-06-28 1982-06-28 Glucose isomerase immobilisation

Publications (2)

Publication Number Publication Date
ES513529A0 ES513529A0 (en) 1983-06-01
ES8306790A1 true ES8306790A1 (en) 1983-06-01

Family

ID=8484384

Family Applications (1)

Application Number Title Priority Date Filing Date
ES513529A Expired ES8306790A1 (en) 1982-06-28 1982-06-28 Glucose isomerase immobilisation

Country Status (1)

Country Link
ES (1) ES8306790A1 (en)

Also Published As

Publication number Publication date
ES513529A0 (en) 1983-06-01

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970505