ES8306790A1 - Glucose isomerase immobilisation - Google Patents
Glucose isomerase immobilisationInfo
- Publication number
- ES8306790A1 ES8306790A1 ES513529A ES513529A ES8306790A1 ES 8306790 A1 ES8306790 A1 ES 8306790A1 ES 513529 A ES513529 A ES 513529A ES 513529 A ES513529 A ES 513529A ES 8306790 A1 ES8306790 A1 ES 8306790A1
- Authority
- ES
- Spain
- Prior art keywords
- pref
- matrix
- immobilisation
- enzyme
- glucose isomerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The immobilisation of glucose isomerase is enhanced by first impregnating the matrix with at least 0.1 mmole Mg(2+) per g of matrix. Pref. amt. of Mg(2+) is 0.1-2 mmole/g. Impregnation is pref. by contact with a soln. of (in)organic Mg salt, pref. the halide, sulphate, nitrate, hypophosphite, fluorosilicate, acetate or lactate. The soln. is pref. 1-25 mM in Mg(2+). MgSO4 is the pref. salt. - Pref. the support is an inorganic oxide impregnated with a polyamine which is then crosslinked with excess of a bifunctional reagent to provide pendant functional gps. - The half-life of the immobilised enzyme is increased, and time for the immobilisation may be decreased. The enzyme may be used for converting glucose to fructose. DEAB- DE3224024 C Glucose isomerase is immobilised on a carrier matrix by treating the matrix with at least 0.1 mmoles of divalent Mg ions, pref. 0.1-2m moles MgSO4 per Gram carrier matrix before contact with the enzyme.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES513529A ES8306790A1 (en) | 1982-06-28 | 1982-06-28 | Glucose isomerase immobilisation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES513529A ES8306790A1 (en) | 1982-06-28 | 1982-06-28 | Glucose isomerase immobilisation |
Publications (2)
Publication Number | Publication Date |
---|---|
ES513529A0 ES513529A0 (en) | 1983-06-01 |
ES8306790A1 true ES8306790A1 (en) | 1983-06-01 |
Family
ID=8484384
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES513529A Expired ES8306790A1 (en) | 1982-06-28 | 1982-06-28 | Glucose isomerase immobilisation |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES8306790A1 (en) |
-
1982
- 1982-06-28 ES ES513529A patent/ES8306790A1/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
ES513529A0 (en) | 1983-06-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19970505 |