ES8302779A1 - Procedimiento para determinar la desoxirribonucleasa b - Google Patents

Procedimiento para determinar la desoxirribonucleasa b

Info

Publication number
ES8302779A1
ES8302779A1 ES506016A ES506016A ES8302779A1 ES 8302779 A1 ES8302779 A1 ES 8302779A1 ES 506016 A ES506016 A ES 506016A ES 506016 A ES506016 A ES 506016A ES 8302779 A1 ES8302779 A1 ES 8302779A1
Authority
ES
Spain
Prior art keywords
determining
toluidine
blue
deoxyribo
streptococcal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES506016A
Other languages
English (en)
Other versions
ES506016A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Publication of ES8302779A1 publication Critical patent/ES8302779A1/es
Publication of ES506016A0 publication Critical patent/ES506016A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA DETERMINAR LA DESOXIRRIBONUCLEASA B. CONSISTE EN AÑADIR UN COMPLEJO DNA-AZUL DE TOLUIDINA O A UNA DILUCION EN SERIE DE UNA SOLUCION DE DESOXIRRIBONUCLEASA B O DE UNA SOLUCION DE ANTIDESOXIRRIBONUCLEASA B A LA QUE PREVIAMENTE SE HABIA AÑADIDO UNA CANTIDAD EN EXCESO DEFINIDA DE DESOXIRRIBONUCLEASA B, DETERMINANDOSE LA CONCENTRACION LIMITE DE PRECIPITACION. DESPUES DE LA ADICION DEL COMPLEJO, SE AGREGA UNA SOLUCION 0,5 A 5 MOLAR DE UNA BASE Y SE CALIENTA LA MEZCLA DURANTE 2 A 15 MINUTOS A UNA TEMPERATURA DE 40 A 70<025>C. ADEMAS DE LA BASE SE AÑADE DE UN 1 A UN 10% DE UN AMINOACIDO. LA BASE EMPLEADA ES UN HIDROXIDO METALICO. ADICIONALMENTE SE AÑADE DE UN 1 A 10% DE UNA SAL. COMO AMINOACIDOS SE EMPLEAN GLICINA, B-ALANINA, LEUCINA, SARCOSINA, ALAMINA O ACIDO GLUTAMICO.
ES506016A 1980-10-10 1981-10-05 Procedimiento para determinar la desoxirribonucleasa b Granted ES506016A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19803038286 DE3038286A1 (de) 1980-10-10 1980-10-10 Verfahren zur bestimmung der streptokokken-desoxiribonuclease b nach der toluidinblau o-methode

Publications (2)

Publication Number Publication Date
ES8302779A1 true ES8302779A1 (es) 1982-12-01
ES506016A0 ES506016A0 (es) 1982-12-01

Family

ID=6114071

Family Applications (1)

Application Number Title Priority Date Filing Date
ES506016A Granted ES506016A0 (es) 1980-10-10 1981-10-05 Procedimiento para determinar la desoxirribonucleasa b

Country Status (5)

Country Link
US (1) US4481291A (es)
EP (1) EP0049849B1 (es)
JP (1) JPS5794661A (es)
DE (2) DE3038286A1 (es)
ES (1) ES506016A0 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE453020B (sv) * 1983-07-06 1988-01-04 Statens Bakteriologiska Lab Serologisk diagnostik av beta-streptokockinfektioner med anvendning av maskinell avlesning med fotometer for mikrotiterplattor
DE3637253A1 (de) * 1986-11-03 1988-05-05 Behringwerke Ag Latex-agglutinations-verfahren zum nachweis von anti-streptokokken-desoxyribonuclease b
US7005510B1 (en) * 1993-06-23 2006-02-28 Beckman Instruments, Inc. Recombinant DNase B derived from Streptococcus pyogenes
EP1380648A3 (en) * 1993-06-23 2004-04-14 Beckman Coulter, Inc. Recombinant DNAase B derived from streptococcus pyogenes
US5962340A (en) * 1994-11-02 1999-10-05 Wako Pure Chemical Industries Ltd. Homogeneous immunoassay method utilizing 5-300 mM magnesium
US20050231512A1 (en) * 2004-04-16 2005-10-20 Niles Gregory E Animation of an object using behaviors

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3906120A (en) * 1970-10-30 1975-09-16 Gen Electric Method for preparing slides for blood evaluation
NL179658C (nl) * 1973-09-04 1986-10-16 Behringwerke Ag Werkwijze voor het bepalen van de enzymactiviteit van desoxyribonuclease, desoxyribonuclease-antilichamen respectievelijk desoxyribonuclease-b-antilichamen.
DE2915309A1 (de) * 1979-04-14 1980-10-30 Behringwerke Ag Verfahren zur bestimmung von anti-hyaluronidase und hierfuer verwendbares mittel
US4241181A (en) * 1979-05-02 1980-12-23 Minnesota Mining And Manufacturing Company Broth medium for detection of DNAse-positive microorganisms
US4307189A (en) * 1980-03-21 1981-12-22 Malon Kit Method for the quantitative determination of terminal deoxynucleotidyl transferase in biological samples

Also Published As

Publication number Publication date
EP0049849A2 (de) 1982-04-21
EP0049849A3 (en) 1982-06-23
DE3038286A1 (de) 1982-05-19
ES506016A0 (es) 1982-12-01
EP0049849B1 (de) 1984-12-05
DE3167646D1 (en) 1985-01-17
US4481291A (en) 1984-11-06
JPS5794661A (en) 1982-06-12
JPH0248239B2 (es) 1990-10-24

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Legal Events

Date Code Title Description
PC1A Transfer granted